FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1117143
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37090.3320076301780524No Hit
GGGTTACAGTATTCCGTGGACGTTCGGCCAGGGGACCAAAGTGGAAATCAGACGAACTGTGGCTGCACCAT33720.30184139362642026No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29620.2651406310561853No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28680.25672630988154604No Hit
GTAATATATGGCCGTGTCGTCGACTCTCAGGCTGTCCATTTGCAGATACA27190.24338871567919237No Hit
CTCCTGTACAGCCTCTGGCTTCACATTTAGTGATTATGCCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG26440.23667516155049087No Hit
GGACTGAACAGAGCGAACTCACCATGGAGTTTGGGCTGATCTGGCTTTTT24610.22029408947645915No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23650.2117007401917212No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22770.2038235033473781No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20010.17911762415375648No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19850.17768539927296687No Hit
GAGTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGGGTTACAGTATT19140.17132990136446274No Hit
ACAGTATTCCGTGGACGTTCGGCCAGGGGACCAAAGTGGAAATCAGACGA19060.1706137889240679No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18910.16927107809832762No Hit
ATATATTACTGTGCGAGAGGGAGTAGGAATACGTTTGGGGGAGCTATTGG18160.16255752396962608No Hit
CTGTAACCCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAACTCTGTC17980.1609462709787377No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17770.1590664758227013No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17460.15629154011617136No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15990.14313297402391637No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15900.14232734752847218No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15790.14134269292292928No Hit
GTAGGAGACAGGGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAG15780.14125317886787994No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15230.1363299058401655No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14810.13257031552809265No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14760.1321227452528459No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGGGTCACCATCACTT14730.1318542030876978No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14450.12934780954631592No Hit
GAATACTGTAACCCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAACT14430.1291687814362172No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC14410.12898975332611848No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14310.12809461277562495No Hit
GTTTAGGGGCTTTCCCTGATTTATGCTGATACCAATTTAAATAACTGCTA13910.12451405057365082No Hit
GCATAAATCAGGGAAAGCCCCTAAACTCCTCATCTATGCTGCAGTAAGTTTGCAAAGTGGGGTCCCATCCAGGTT13510.12093348837167667No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13230.11842709483029477No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13190.11806903861009735No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT13020.11654729967425835No Hit
CCCCTAAACTCCTCATCTATGCTGCAGTAAGTTTGCAAAGTGGGGTCCCA13010.11645778561920901No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12710.1137723639677284No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACTTTGGTCCCCTGGCCGAACGTC12660.11332479369248162No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12610.11287722341723486No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12540.11225062503188937No Hit
ATCTGGGACAGAGTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATT12520.11207159692179067No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACTGGC12460.11153451259149456No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC12420.11117645637129714No Hit
ACTTACTACTGTCAACAGGGTTACAGTATTCCGTGGACGTTCGGCCAGGGGACCAAAGTGGAAATCAGACGAA12350.11054985798595167No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG12290.11001277365565554No Hit
GAGTGAACTCTGTCCCAGATGCACTGCCACTGAACCTGGATGGGACCCCA11790.10553707090318787No Hit
CAGTAATATATGGCCGTGTCGTCGACTCTCAGGCTGTCCATTTGCAGATA11640.10419436007744756No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11640.10419436007744756No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCACTTTGGTCCCCTGGCCGAACGTCCACGGAATA11600.10383630385725014No Hit
GTGGGGTCCCATCCAGGTTCAGTGGCAGTGCATCTGGGACAGAGTTCACT11490.10285164925170727No Hit
CTCTTATTAGTGCAAGTGGTGTAACCACTTACTACGCAGACTCCGTGAAG11470.10267262114160855No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11430.10231456492141114No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11400.10204602275626308No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCTA200.007764511751.0525528
GTATCAA27600.035.4034351
AATGGTA3600.032.3775270
TCTGGAG13550.031.54120470
ACGACTG553.611102E-430.8162845
GTGGTAT4550.030.5727441
TATCAAC32150.030.4698562
ATACGTA1450.030.39259511
GTACGGA450.005016287330.1584781
ATTCACT1351.0913936E-1130.130132
GTAGTTT1104.7221375E-429.4341170
ATCAACG33000.029.271543
TCAACGC33550.028.791684
CAACGCA34150.028.289625
AACGCAG34200.028.2495256
TAGTCAC1103.734567E-828.17098438
GATTCAC1504.3655746E-1127.1426331
CGCAGAG36050.026.7998288
TGTTAGA11050.026.37083470
GCAGAGT36800.026.2536359
Produced by FastQC (version 0.11.5)