FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences868741
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCTTCGGACCGGAGCCCACTGATGGCCAGGGAGG45890.5282356881970576No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTGTGGTATTCGG42000.4834582458983748No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35720.41116972722595113No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC32750.37698232269456605No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAATACCACACCACTCAGGCTGTCATCCCATG31550.3631692299546125No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT26540.30549956776530635No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26320.3029671674296482No Hit
GTAATAGACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACA26280.30250673100498304No Hit
GACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAATACCACACCACTCAGG25980.29905345781999465No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23770.2736143453572469No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23680.27257836340175035No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCC22700.26129767099745493No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22020.2534702517781479No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC21420.24656370540817116No Hit
GTACTACCTCGCGCACAGTAATAGACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACAGTGAGTT20950.241153577418356No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19780.22768581199690127No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18910.21767131976043494No Hit
AGGTAGTACCGGTCACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCC18780.2161749013802733No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC18380.21157053713362212No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17850.2054697545068093No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17670.20339779059581625No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16910.194649498527179No Hit
ATACCACACCACTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCC16850.1939588438901813No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16700.1922322072976871No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16520.19016024338669407No Hit
GTGTGGTATTCGGCGGGGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC16240.18693718841403825No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16080.18509544271537778No Hit
GTCTATTACTGTGCGCGAGGTAGTACCGGTCACTTTGACTACTGGGGCCA15900.18302347880438474No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15670.18037596936256028No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA15650.18014575115022774No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15360.17680758707140562No Hit
CCCAGGGACGGCCCCCAAACTCCTCATCTATGGTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG15280.17588671422207539No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14900.17151256818775676No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14750.16978593159526256No Hit
ACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAATACCACACCACTCAGGC14070.16195851237595554No Hit
CCCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACTCACTGTATCTG13910.16011676667729507No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13630.15689371170463923No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13550.15597283885530902No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG13430.15459152958131364No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13260.1526346747764869No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12980.14941161980383105No Hit
GCATGGGATGACAGCCTGAGTGGTGTGGTATTCGGCGGGGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG12760.1468792194681729No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12740.14664900125584035No Hit
CAGTAATAGACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATA12730.14653389214967408No Hit
CCCCTGAGGGCCGCTGATCATTACCATAGATGAGGAGTTTGGGGGCCGTCCCTGGGAGGTGCTGGTACCAGGAGA12700.14618856483117523No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT12650.14561301930034382No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC12420.1429655098585194No Hit
GTCCCTGGGAGGTGCTGGTACCAGGAGACATAATTACCTCCGATGTTGGA12290.14146909147835776No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12240.14089354594752637No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12210.14054821862902753No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11970.1377856000810368No Hit
GTATATGGGACTACTACTACTAGTAATGTATGAAAGCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT11840.13628918170087517No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT11820.1360589634885426No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG11380.1309941628172263No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCTTCGGACCGGAGCCCAC11370.13087905371106004No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAATACCACAC11360.13076394460489377No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG11270.12972796264939723No Hit
GTGCTGGTACCAGGAGACATAATTACCTCCGATGTTGGAGTTGCTTCCAG11110.12788621695073676No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11030.12696534410140653No Hit
GTACCAGCACCTCCCAGGGACGGCCCCCAAACTCCTCATCTATGGTAATGATCAGCGGCCCTCAGGGGTCCCTGA10740.12362718002258441No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10720.12339696181025185No Hit
GTTTGGGGGCCGTCCCTGGGAGGTGCTGGTACCAGGAGACATAATTACCT10680.12293652538558673No Hit
CTCTTGTTCTGGAAGCAACTCCAACATCGGAGGTAATTATGTCTCCTGGTACCAGCACCTCCCAGGGACGGCCCC10680.12293652538558673No Hit
TCCCAGGGACGGCCCCCAAACTCCTCATCTATGGTAATGATCAGCGGCCC10580.12178543432392393No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10510.12097967058075998No Hit
TCCCTGGGAGGTGCTGGTACCAGGAGACATAATTACCTCCGATGTTGGAGTTGCTTCCAGAACAAGAGATGGTGA10440.12017390683759602No Hit
GATCATTACCATAGATGAGGAGTTTGGGGGCCGTCCCTGGGAGGTGCTGG10440.12017390683759602No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC10420.11994368862526346No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC10400.1197134704129309No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10380.11948325220059834No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG10360.11925303398826578No Hit
GGCCATCAGTGGGCTCCGGTCCGAAGATGAGGCTGATTATTACTGTGCAG10140.11672063365260761No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC10130.11660552454644134No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10100.11626019722794251No Hit
GTATTCGGCGGGGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC10030.11545443348477856No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10020.11533932437861227No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA9940.11441845152928203No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT9910.1140731242107832No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9870.11361268778611808No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG9850.11338246957378552No Hit
GTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG9640.11096517834429363No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9430.10854788711480176No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC9430.10854788711480176No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9280.10682125052230756No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG9250.10647592320380873No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT9240.10636081409764245No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC9140.10520972303597964No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGA9030.10394352286815058No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC9010.10371330465581803No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAAGATG8810.10141112253249242No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG8810.10141112253249242No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC8700.10014492236466335No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGT100.009753441130.3229270
TTTCGTT150.00245510968.2819128
CTTAGAC150.002466911268.199383
GATTAGG200.00701972852.3703249
GGTATCA5600.048.74471
TGGTATC1100.046.5022542
TTAGACA405.3575284E-542.624614
GTATCAA16500.038.877591
TCTGGAG8600.037.8845770
TCGTATG1750.037.41880440
GCTTGTA557.973791E-637.223231
CGTATGC1800.036.4323841
CGAGACG1900.035.90060823
CTTATAC2050.034.953521
GCCAGAG18050.034.29550670
TATCAAC18650.034.1930772
CCCATTG400.002725679734.15867229
GACGGAC1900.034.12129226
GTACGGA601.4475432E-534.1212921
TTATACA1900.034.1016542
Produced by FastQC (version 0.11.5)