FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences868741
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTGTGGTATTCGG42070.48426400964153876No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC38110.43868080359969197No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33290.38319821442754515No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT28670.33001780737872394No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26960.31033415022429006No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAATACCACACCACTCAGGCTGTCATCCCATG24510.28213241921355153No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC23830.2743049999942445No Hit
GTAATAGACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACA23730.27315390893258173No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22030.2535853608843142No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21820.2511680696548223No Hit
GACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAATACCACACCACTCAGG20520.23620388585320595No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20200.23252039445588502No Hit
GTACTACCTCGCGCACAGTAATAGACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACAGTGAGTT20190.23240528534971874No Hit
GTAATAATCAGCCTCATCTTCGGACCGGAGCCCACTGATGGCCAGGGAGG19990.23010310322639313No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA19560.22515341166124309No Hit
AGGTAGTACCGGTCACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCC19280.22193035668858727No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19150.22043393830842567No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC18910.21767131976043494No Hit
CCCAGGGACGGCCCCCAAACTCCTCATCTATGGTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG18830.2167504469111047No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCC18620.2143331556816128No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18250.21007411875346046No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18100.20834748216096627No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17590.20247691774648602No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17460.20098049936632437No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17290.19902364456149763No Hit
GTGTGGTATTCGGCGGGGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC17090.196721462438172No Hit
GTCTATTACTGTGCGCGAGGTAGTACCGGTCACTTTGACTACTGGGGCCA16810.1934984074655162No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16670.19188687997918827No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16400.18877893411269872No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15450.17784356902690215No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG14870.17116724086925794No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14110.16241894880062066No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13960.16069231220812646No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13770.15850523919096715No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13750.1582750209786346No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13650.1571239299169718No Hit
CCCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACTCACTGTATCTG13510.15551240243064388No Hit
CTCTTGTTCTGGAAGCAACTCCAACATCGGAGGTAATTATGTCTCCTGGTACCAGCACCTCCCAGGGACGGCCCC13280.15286489298881945No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC13180.15171380192715664No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT13070.1504476017593276No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12750.14676411036200662No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12640.14549791019417754No Hit
ATACCACACCACTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCC12580.14480725555717988No Hit
GTACCAGCACCTCCCAGGGACGGCCCCCAAACTCCTCATCTATGGTAATGATCAGCGGCCCTCAGGGGTCCCTGA12560.1445770373448473No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12460.1434259462831845No Hit
GCATGGGATGACAGCCTGAGTGGTGTGGTATTCGGCGGGGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG12460.1434259462831845No Hit
CCCCTGAGGGCCGCTGATCATTACCATAGATGAGGAGTTTGGGGGCCGTCCCTGGGAGGTGCTGGTACCAGGAGA12440.14319572807085196No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG12430.14308061896468569No Hit
GTATATGGGACTACTACTACTAGTAATGTATGAAAGCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT12270.1412388732660252No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT11780.13559852706387748No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11690.13456254510838098No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11630.1338718904713833No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11600.13352656315288447No Hit
GTGCTGGTACCAGGAGACATAATTACCTCCGATGTTGGAGTTGCTTCCAG11470.13203014477272282No Hit
GTTTGGGGGCCGTCCCTGGGAGGTGCTGGTACCAGGAGACATAATTACCT11260.12961285354323096No Hit
GTCCCTGGGAGGTGCTGGTACCAGGAGACATAATTACCTCCGATGTTGGA11250.12949774443706466No Hit
TCCCAGGGACGGCCCCCAAACTCCTCATCTATGGTAATGATCAGCGGCCC11130.1281164351630693No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT11100.12777110784457046No Hit
GTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG11080.12754088963223792No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG10990.12650490767674138No Hit
CAGTAATAGACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATA10880.12523870750891233No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10800.12431783465958207No Hit
ACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAATACCACACCACTCAGGC10690.12305163449175302No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG10540.12132499789925882No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC10480.12063434326226112No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC10380.11948325220059834No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10240.11787172471427042No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10230.11775661560810415No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10210.11752639739577159No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAACTCCAACATCGGAGGTAATTAT10040.11556954259094483No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC9920.11418823331694947No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9890.11384290599845064No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG9890.11384290599845064No Hit
GATCATTACCATAGATGAGGAGTTTGGGGGCCGTCCCTGGGAGGTGCTGG9880.11372779689228435No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAAGATG9860.1134975786799518No Hit
GGCCATCAGTGGGCTCCGGTCCGAAGATGAGGCTGATTATTACTGTGCAG9860.1134975786799518No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG9810.11292203314912039No Hit
GCCCCCAAACTCCTCATCTATGGTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT9690.11154072387512505No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9650.1110802874504599No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9300.10705146873464012No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA9240.10636081409764245No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTACCTATAGCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG9230.10624570499147618No Hit
GTATTCGGCGGGGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC9200.10590037767297733No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAATACCACAC9150.10532483214214593No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC9050.10417374108048313No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC9050.10417374108048313No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC9040.10405863197431686No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGA9040.10405863197431686No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT9020.1038284137619843No Hit
TCCCTGGGAGGTGCTGGTACCAGGAGACATAATTACCTCCGATGTTGGAGTTGCTTCCAGAACAAGAGATGGTGA9000.10359819554965174No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAA8910.10256221359415522No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG8880.10221688627565638No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8850.10187155895715755No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8690.10002981325849707No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAAC200.00773999151.0924641
TGGTATC1950.045.3841482
TCTGGAG9000.045.20878670
TAATACT551.2856617E-744.66079751
GGTATCA5800.044.0452271
TCGGTTT650.00110517441.0470770
CGGAGTA350.001195134440.4033256
GTATCAA17250.039.491761
ACACTGT451.08480315E-437.815776
CCGTTAA656.023165E-736.68176716
ACGGGAT754.4976332E-836.3324216
TATCAAC19200.034.924522
GCCGTTA701.0780259E-634.05183415
ATCAACG20200.033.187943
TCAACGC20350.032.9433144
AACGCAG20650.032.7980846
CAACGCA20600.032.7105945
GTTAGGG751.8437677E-631.7908671
AGAGTAC21850.031.15437311
CGCAGAG22050.030.8717948
Produced by FastQC (version 0.11.5)