FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1183590
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAACTGTGGCTGCACCAT83390.7045514071595738No Hit
CTATAATATTGCTGACAGTAGTAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC65390.552471717402141No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54310.45885821948478783No Hit
CTTTTATACAGTTCCTACAATAACAACTACTTAGCTTGGTTGCAGCAGAA52620.4445796263908955No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49390.41728977095108943No Hit
CCTTAGGAGGCTGACCTGGTTTCTGCTGCAACCAAGCTAAGTAGTTGTTATTGTAGGAACTGTATAAAAGACT45400.38357877305485855No Hit
CTCCTAAGGTGCTCATTTACTGGGCATCCACCCGGGAATTCGGGGTCCCT38660.3266333781123531No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36430.3077923943257378No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34250.28937385412178207No Hit
GTATAAAAGACTCTGGTTGGACTTGCACTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA32010.2704483816186348No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29210.2467915409897008No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28690.24239812773004166No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28570.24138426313165878No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27790.23479414324217002No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27630.23344232377765947No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27380.2313301058643618No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26800.2264297603055112No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26390.222965722927703No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC25150.2124891220777465No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24640.20818019753461925No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24180.2042937165741515No Hit
GTTTACTACTGTCAGCAATATTATAGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAA23330.1971121756689394No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCAACCAGAGTCTTTTATA23070.19491546903910983No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22340.1887477927322806No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT21490.1815662518270685No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19390.163823621355368No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19010.16061305012715552No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18890.1595991855287726No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCAACCAGAGTCTTTTATACAGTTCCTA18090.15284008820622008No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17470.1476017877812418No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCAAC17150.1448981488522208No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17130.14472917141915698No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTAATACCTGGATGAGGTGGGTCCGCCAGGCTCCAGGAAAGGGG17080.14430672783649745No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17070.14422223911996554No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16280.13754763051394486No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATGTGGCAGTTTACTACTGTCAGCAATATTATAGTGCT16240.13720967564781725No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC16130.13628029976596626No Hit
CATTTACTGGGCATCCACCCGGGAATTCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCA16100.13602683361637052No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16100.13602683361637052No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15990.13509745773451956No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT15940.13467501415186003No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC15900.1343370592857324No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15610.1318868865063071No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15570.13154893164017945No Hit
GTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGC15470.1307040444748604No Hit
GTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCCCTTGGCCGAACGTC15420.13028160089220084No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15310.12935222501034987No Hit
GTAGTAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA15260.12892978142769032No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14870.12563472148294594No Hit
GTCTCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCAACCAGAGTCTTTTATACAGTTCCTACAATAACAACTA14870.12563472148294594No Hit
GTGCAAGTCCAACCAGAGTCTTTTATACAGTTCCTACAATAACAACTACT14840.12538125533335023No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT14690.12411392458537163No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14270.12056539849103153No Hit
GGATCTCTGGTGCCTACGGGGACGTGGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG14150.11955153389264864No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14140.11946704517611673No Hit
ATAGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGA14090.1190446015934572No Hit
GGCTGAGGATGTGGCAGTTTACTACTGTCAGCAATATTATAGTGCTCCGT14020.11845318057773385No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13900.11743931597935095No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13870.11718584982975523No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13600.11490465448339374No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC13580.11473567705032994No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATGTGGCAGTTTA13550.1144822109007342No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13420.11338385758581941No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCC13260.11203203812130891No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13220.11169408325518128No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13160.11118715095598984No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13090.11059572994026648No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13020.11000430892454313No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12650.10687822641286256No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12530.10586436181447968No Hit
GTAATAGACGCCTGTGTCGTCGGTTTTCAGGCTGTTCATTTGCAGATAAA12380.10459703106650106No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12250.10349867775158628No Hit
GTACTGGTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCG12240.10341418903505437No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12230.10332970031852245No Hit
CTACTTAGCTTGGTTGCAGCAGAAACCAGGTCAGCCTCCTAAGGTGCTCATTTACTGGGCATCCACCCGGGAATT12170.10282276801933102No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG12090.10214685828707575No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12030.10163992598788431No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11980.1012174824052248No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTCCTTGCTGCTATTTTAAAAG11920.10071055010603333No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11880.10037259523990572No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAC150.002107410270.9641260
CGACCGT200.00766912551.2126410
CGCAACC200.007672969351.20614618
TCGTATG4050.047.74701740
ATCTCGT4000.047.32887637
ACTCGAC4450.046.10396227
GACTCGA4500.045.5801226
CGTATGC4350.045.29633741
GACGTCA4550.045.1901331
ATCTCCG4700.045.03806710
CCGTCTT4450.044.58361447
TATACAC4700.044.3041573
TCGACGT4700.043.69629
ACGAGAC4700.043.59443322
TCTCCGA4700.043.58522411
GTGGTAT2900.043.571221
CTTATAC4650.043.330641
CTCGACG4750.043.21224628
AGACTCG4750.043.16290725
ACGTCAT4700.043.04249232
Produced by FastQC (version 0.11.5)