FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1183590
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAACTGTGGCTGCACCAT84290.7121553916474455No Hit
CTATAATATTGCTGACAGTAGTAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC79960.6755717773891297No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52980.44762122018604417No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45700.3861134345508157No Hit
CTTTTATACAGTTCCTACAATAACAACTACTTAGCTTGGTTGCAGCAGAA43290.3657516538666261No Hit
CCTTAGGAGGCTGACCTGGTTTCTGCTGCAACCAAGCTAAGTAGTTGTTATTGTAGGAACTGTATAAAAGACT42930.3627100600714775No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41170.3478400459618618No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34880.2946966432632922No Hit
CTCCTAAGGTGCTCATTTACTGGGCATCCACCCGGGAATTCGGGGTCCCT32070.27095531391782623No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31310.26453417146140135No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29660.25059353323363665No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28150.23783573703731867No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26960.2277815797700217No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25750.21755844506966093No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25660.21679804662087376No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24690.2086026411172788No Hit
GTATAAAAGACTCTGGTTGGACTTGCACTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA24410.2062369570543854No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC24370.20589900218825774No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23400.19770359668466278No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23360.1973656418185351No Hit
GTAGTAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA22960.19398609315725884No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22110.18680455225204676No Hit
GTTTACTACTGTCAGCAATATTATAGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAA21960.18553722150406812No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21780.1840164246064938No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21570.18224216155932374No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21140.17860914674845174No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20560.1737088011896011No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19730.16669623771745282No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCAACCAGAGTCTTTTATA18780.15866980964692165No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTAATACCTGGATGAGGTGGGTCCGCCAGGCTCCAGGAAAGGGG17460.14751729906470992No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16390.13847700639579585No Hit
GTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCCCTTGGCCGAACGTC16350.1381390515296682No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16280.13754763051394486No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGAATTCCCGGGT16260.13737865308088104No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16260.13737865308088104No Hit
TCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC16260.13737865308088104No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16180.1367027433486258No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG15970.13492848030145574No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15850.13391461570307286No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCAACCAGAGTCTTTTATACAGTTCCTA15790.13340768340388143No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15790.13340768340388143No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15670.13239381880549853No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATGTGGCAGTTTACTACTGTCAGCAATATTATAGTGCT15500.1309575106244561No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC15470.1307040444748604No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14970.12647960864826502No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14500.12250863897126539No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCAAC14450.12208619538860584No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT14440.12200170667207395No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14400.12166375180594632No Hit
GTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGC14370.1214102856563506No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC14010.11836869186120193No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC13950.11786175956201049No Hit
GGCTGAGGATGTGGCAGTTTACTACTGTCAGCAATATTATAGTGCTCCGT13810.1166789175305638No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT13470.11380630116847895No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13350.11279243657009606No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13200.11152510582211744No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG12930.10924391047575596No Hit
GGATCTCTGGTGCCTACGGGGACGTGGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG12910.10907493304269215No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12900.10899044432616024No Hit
CATTTACTGGGCATCCACCCGGGAATTCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCA12880.10882146689309644No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12700.1073006699955221No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12600.10645578283020303No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12560.1061178279640754No Hit
ATAGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGA12540.10594885053101159No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12540.10594885053101159No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12530.10586436181447968No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12440.1051039633656925No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12220.10324521160199056No Hit
GAGCACTATAATATTGCTGACAGTAGTAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT12200.10307623416892674No Hit
GTCTCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCAACCAGAGTCTTTTATACAGTTCCTACAATAACAACTA12060.10189339213748003No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11990.10130197112175668No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11950.10096401625562908No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATGTGGCAGTTTA11940.10087952753909715No Hit
GTGCAAGTCCAACCAGAGTCTTTTATACAGTTCCTACAATAACAACTACT11890.10045708395643761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9100.040.035571
GTATCAA24350.039.9917761
ATGTCGG350.001437900238.9130741
TGGTATC3150.035.643192
TATCAAC27700.035.1284872
AATGGTA4250.034.5337470
GCTTATA400.002769420934.0489431
GTGGTAT3300.034.048941
ATCAACG28900.033.5493053
TCAACGC29400.033.2101674
CAACGCA30050.032.2694785
AACGCAG29950.032.264986
ACGCAGA31550.030.6287217
GAGTTTA2450.030.574561
CGCAGAG32850.029.9345238
AGAGTAC33900.029.20809211
ACGATGG755.1805767E-428.97409469
GCAGAGT34250.028.7109249
GATATTA953.621717E-728.6485653
CATGACG500.00661099828.50211161
Produced by FastQC (version 0.11.5)