FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1286856
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51420.399578507618568No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45450.35318637050299334No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42560.33072853528289103No Hit
GTCCTAATCTCTGCTGCATCCACTTTGCAAAGTGGGGTCCCATCGAGGTT33660.26156772785766236No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33080.2570606190591644No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32710.2541853944808122No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30730.23879905754800845No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29820.23172755926070981No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25560.19862362222346558No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25050.1946604748316828No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24690.19186295902571848No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24530.19061961866751212No Hit
GATTAGGACCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAT23710.18424749933170456No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22300.17329056242501104No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22110.171814095749641No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22070.1715032606600894No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22020.1711147167981499No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21760.16909428871606458No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20980.1630330044698086No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTGGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGGACATTAGTA20590.16000236234668058No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19930.1548735833690794No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18920.14702499735790173No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18820.14624790963402276No Hit
GTGGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGGACATTAGTACTTATTTAGCCTGGTATCAGCAAAAA18020.14003120784299097No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17950.1394872464362757No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17920.139254120119112No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG17840.13863244994000884No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTGACGCCTGG17730.13777765344374196No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAAAA17370.13498013763777764No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17170.1334259621900197No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGGTGGA17030.13233803937658914No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAAAACTATTGAG16840.1308615727012191No Hit
CATTAGTACTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGGTCCTAATCTCTGCTGCATCCA16550.12860801830197008No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16530.12845260075719428No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTA16430.12767551303331529No Hit
ACTGTCAACAGCTCAATAGTTTTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT15900.12355694809675674No Hit
GTGGGGTCCCATCGAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT15890.12347923932436884No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15240.11842816911915553No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15100.11734024630572497No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14910.11586377963035491No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT14870.11555294454080331No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14870.11555294454080331No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTGACGCCTGGATGAACTGGGTCCGCCAGGCTCCAGGGAGGGGG14830.11524210945125174No Hit
ACTTATTACTGTCAACAGCTCAATAGTTTTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCA14810.11508669190647594No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14740.11454273049976067No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14390.11182292346618426No Hit
GTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCA14110.10964707783932312No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13970.10855915502589256No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13820.1073935234400741No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13540.10521767781321298No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC13440.104440590089334No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13320.10350808482067925No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13200.10257557955202447No Hit
GGACATTAGTACTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGGTCCTAATCTCTGCTGCATC12960.10071056901471494No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12940.10055515146993914No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12890.10016660760799964No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG12880.10008889883561176No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGAC200.006411957653.58480562
GTGGTAT2900.039.918151
GTATCAA29800.036.4471781
TACGTAT750.002747672834.10170470
TGTTAGA13900.032.2003570
TATCAAC34950.030.762772
GGTACGT500.00456242230.74902268
GTCGCGT450.00495074930.2400448
GGGCGTA450.0049516930.2388694
ATCAACG35750.030.0697023
TCAACGC35900.029.84934
CAACGCA36850.029.357875
AACGCAG37350.028.964866
ACGCAGA38600.027.9387457
CGCAGAG39050.027.6167898
CCACTAG500.008269886527.22450414
AGAGTAC40150.026.52123511
GCAGAGT41350.026.0806679
CAGAGTA40900.026.03490310
GAGTTTA4500.025.7250291
Produced by FastQC (version 0.11.5)