FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797741
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25290.3170201857495102No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23450.2939550555882172No Hit
GTATAGAGTATCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA21070.2641208111404579No Hit
ACTCTATACGTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACC19270.24155709685223647No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCTTC17170.21523276351597823No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16440.20608192383242183No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16020.20081705716517012No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15470.19392258891043584No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15040.1885323682749163No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14730.1846463952586115No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14620.18326750160766464No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14220.17825334287694877No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13380.16772360954244547No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12270.15380931906470896No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12230.1533079031916374No Hit
GGTCTAGTCAGAGCCTCTTCGATAGTGATAATGGAAACACCTATTTGGACTGGTACCTCCAGAAGCCAGGGCAGT12130.15205436350895843No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12110.15180365557242265No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT11700.14666414287343887No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11670.1462880809686352No Hit
GTCCCAGACAGGTTCAGTGGCAGTGGGTCAGGCACTGATTTCACACTGAA11670.1462880809686352No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11530.14453312541288463No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11260.14114856826965141No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10930.13701188731681083No Hit
GTCTGGGACTCCAGAGGCCCGATAGGAAAGCGTGTAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGGAGGTA10710.13425410001491714No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG10430.13074418890341602No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACCCTACTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCA10300.12911458731593337No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10230.12823710953805809No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10190.1277356936649865No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT10140.12710892382364702No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9910.1242257825534854No Hit
GTAATAGAAGGCGGTGTCCTCATCTCTGAGACTGTTCATTTGCAGATAGA9760.12234547302946695No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG9580.12008910160064483No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC9380.1175820222352869No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCTGGATCCAGTGAGGGTGTGGTGTTGA9220.11557635874300054No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9200.11532565080646474No Hit
GTGTTGACCCAGACTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGC8860.11106361588535628No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8750.1096847222344094No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8730.10943401429787361No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGAGGATACT8620.10805512064692675No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG8610.10792976667865888No Hit
GTTTATTACTGCATGCAACGTATAGAGTATCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA8600.10780441271039097No Hit
CTCTATACGTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACCC8590.10767905874212308No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8310.10416914763062197No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA8220.10304096191621091No Hit
GAGTATCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT8190.10266490001140721No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC8160.10228883810660352No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAT200.007680106451.1925663
TCTTATA1200.048.3485342
TGGTATC2250.047.021322
GTGGTAT2300.046.025081
GGTATCA8850.045.530191
CGCGTAT551.4681427E-743.91231539
ATGCCGT2400.043.39215544
ATCTCCG3550.043.2586110
TCTCCGA3550.043.2586111
TCGTATG2000.043.17741840
CGAGACT3500.042.91769423
GCCGTCT2050.042.44476346
ACGAGAC3550.042.3105722
ATCTCGT2050.041.9460137
AAAACCG600.00106535641.35429870
GTATCAA26500.041.106031
CCCACGA3700.040.5876419
CACGAGA3750.040.05400521
CGTATGC2600.039.89151441
TATACAC3850.039.8903123
Produced by FastQC (version 0.11.5)