FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854756
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31190.36489945668705454No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29860.3493394606180009No Hit
GATTATTACTGTGCAGCATGGGATAACAGCCTGAGTGCTTGGGTATTCGG24950.29189616685931424No Hit
GTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCC17190.2011100243812269No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16600.19420746973405276No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16020.1874219075385256No Hit
GTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC15910.1861349905704084No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15590.18239123211770378No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15460.1808703302462925No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14430.16882010772664946No Hit
AGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG14070.16460837946735676No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT13420.15700387011030048No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCT12900.15092026262465544No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG12640.14787845888183296No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12370.14471966268736342No Hit
GGATAACAGCCTGAGTGCTTGGGTATTCGGCGGCGGGACCAAGCTGACCGTCCTCGGTCAGCCCAAGGCTGCCCC12270.14354973817089323No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11560.1352432741039548No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11430.13372237223254355No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11310.1323184628127793No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11010.12880868926336875No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10680.12494793835901709No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10610.12412899119748794No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10150.11874733842172504No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10080.1179283912601959No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9300.10880298003172835No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG9290.10868598758008133No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCGCCGCCGAATACCCAAGCACTCAGGCTGTTATCCCATG9260.10833501022514028No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9180.10739907061196412No Hit
CTACTATAGATGACGAGCTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCA9140.10693110080537604No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9090.10634613854714095No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA8980.10505922157902371No Hit
CTATAGTAGTAATGCGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCT8830.10330433480431843No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCAC8770.10260238009443631No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8690.10166644048126014No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8610.100730500868084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACT200.00693708752.5272949
GGTATCA12650.039.5059661
GTGGTAT4050.039.4528851
GTATCAA31900.038.685821
TGGTATC4550.035.82682
CGTACAC400.002807613533.9528773
TATCAAC39400.031.2025832
ACGAACC1259.822543E-1130.9842752
ATCAACG40000.030.5575873
AACGCAG40650.030.0724876
TCAACGC40550.030.057634
CAACGCA41000.029.7312095
CAAACGA1301.6552804E-1029.63077749
TAATCGT1302.0008883E-1029.15059338
TTAATCG1302.0372681E-1029.11775237
ACGCAGA42650.028.5809967
ACAAACG1352.6011548E-1028.5075448
CGCAGAG43250.028.3415158
GCTTGTA605.907817E-428.3305261
CAGAGTA44050.027.98096710
Produced by FastQC (version 0.11.5)