FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences918765
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCAGTATGGTGGCTCACCGTACACTTTTGGCCAGGGGA49570.5395286063356789No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35990.3917214957034715No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32860.35765402469619545No Hit
CAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA27540.2997502081598668No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23110.2515333082997284No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21690.2360777783219866No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21250.23128874086409476No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19650.2138740591990335No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT19070.20756123709544877No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18980.2065816612517891No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18710.20364293372081002No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGGAGCAATTACTTAGCCTGGTACC18650.20298988315837022No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGGAG18130.1973301116172253No Hit
CATCTATGGTGCATCCAGAAGGGCCACCGACATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA17580.19134381479486048No Hit
GTATGGTGGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT17360.18894929606591457No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17200.18720782789940846No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16630.18100384755623036No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGTCGGTGGCCCTTCT16560.18024195523338393No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA16460.17915353762931763No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16330.1777385947440314No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16120.1754529177754921No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC15110.16445989997442217No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14420.15694981850636452No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14190.15444645801701196No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA14030.15270498985050585No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCAGAAGGGCCACCGACATCCCA13830.15052815464237318No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13540.14737174359058083No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13270.14443301605960177No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG13070.1422561808514691No Hit
GGCCAAAAGTGTACGGTGAGCCACCATACTGCTGACAATAATACACTGCA12790.13920861156008338No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12690.13812019395601705No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12690.13812019395601705No Hit
CCATAGATGAGGAGCCTGGGAGTCTGGCCAGGTTTCTGATGGTACCAGGCTAAGTAATTGCTCCTAACACTCTGA12600.13714061811235737No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT12310.133984207060565No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12090.13158968833161908No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12070.1313720048108058No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTATTGTCAGCAGTATGGTGGCTCA11900.12952169488389303No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11840.12886864432145326No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC11760.12799791023820017No Hit
GCCTGGTACCATCAGAAACCTGGCCAGACTCCCAGGCTCCTCATCTATGGTGCATCCAGAAGGGCCACCGACATC11740.12778022671738692No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11700.1273448596757604No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC11600.12625644207169406No Hit
GTCTATAGCCCCTCCATCAAAGTGATTTTTAAAGAGGCCGACCCACTCCGGCCCCTTCCCTGGAGCCTGGCGGAC11560.12582107503006754No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11560.12582107503006754No Hit
GTGTTAGGAGCAATTACTTAGCCTGGTACCATCAGAAACCTGGCCAGACTCCCAGGCTCCTCATCTATGGTGCAT11540.12560339150925426No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11450.12462381566559458No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11440.12451497390518794No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT11040.12016130348892264No Hit
CCCTAAGACTCTCCTGTGCAGGCTCTGGAGTCATTCTCAGAGATGTCTGG10940.1190728858848563No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10710.11656952539550376No Hit
CCACCATACTGCTGACAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC10520.11450153194777772No Hit
GTGTATTATTGTCAGCAGTATGGTGGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA10510.11439269018737108No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10470.11395732314574455No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC10300.1121070132188318No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGTCG10220.11123627913557874No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10220.11123627913557874No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10130.11025670329191904No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC10050.10938596920866599No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC9980.10862407688581954No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9950.10829755160459964No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9840.10710029224012668No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT9750.106120716396467No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9670.10524998231321395No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9610.10459693175077414No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC9420.10252893830304813No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC9260.10078747013654199No Hit
TCTATGGTGCATCCAGAAGGGCCACCGACATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT9230.10046094485532209No Hit
GTCAGAGTGTTAGGAGCAATTACTTAGCCTGGTACCATCAGAAACCTGGC9220.10035210309491545No Hit
ATCCAGAAGGGCCACCGACATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAG9190.10002557781369555No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)