FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences918765
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCAGTATGGTGGCTCACCGTACACTTTTGGCCAGGGGA47690.519066355379232No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37300.40597976631674043No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32210.3505793102697643No Hit
CAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA25840.2812471088907392No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22000.2394518728945922No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20770.22606433636457637No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20310.22105761538587124No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19570.2130033251157804No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19050.2073435535746355No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT18960.2063639777309758No Hit
CATCTATGGTGCATCCAGAAGGGCCACCGACATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA17850.19428254232583958No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGGAGCAATTACTTAGCCTGGTACC17320.18851392902428804No Hit
GTATGGTGGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT17050.18557520149330894No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17050.18557520149330894No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGGAG16740.18220110692070335No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16240.17675901890037168No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15830.17229650672369975No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15140.16478642525564208No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15070.16402453293279567No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA15040.16369800765157577No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGTCGGTGGCCCTTCT14960.1628272735683227No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC14840.1615211724434431No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14820.16130348892262986No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14180.15433761625660536No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA13880.15107236344440636No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13760.14976626231952675No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13610.14813363591342726No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13370.14552143366366807No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13350.14530375014285482No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG13100.142582706132689No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12610.137249459872764No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCAGAAGGGCCACCGACATCCCA12480.13583451698747775No Hit
GGCCAAAAGTGTACGGTGAGCCACCATACTGCTGACAATAATACACTGCA12240.1332223147377186No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT12060.13126316305039917No Hit
GTGTTAGGAGCAATTACTTAGCCTGGTACCATCAGAAACCTGGCCAGACTCCCAGGCTCCTCATCTATGGTGCAT11980.13039242896714612No Hit
CCATAGATGAGGAGCCTGGGAGTCTGGCCAGGTTTCTGATGGTACCAGGCTAAGTAATTGCTCCTAACACTCTGA11740.12778022671738692No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11610.12636528383210072No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11540.12560339150925426No Hit
GTGTATTATTGTCAGCAGTATGGTGGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA11430.12440613214478131No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTATTGTCAGCAGTATGGTGGCTCA11300.12299118925949508No Hit
GCCTGGTACCATCAGAAACCTGGCCAGACTCCCAGGCTCCTCATCTATGGTGCATCCAGAAGGGCCACCGACATC11210.1220116134158354No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11120.12103203757217569No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10940.1190728858848563No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10590.11526342427062414No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10530.11461037370818436No Hit
CCCTAAGACTCTCCTGTGCAGGCTCTGGAGTCATTCTCAGAGATGTCTGG10520.11450153194777772No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACTGGC10330.11243353850005171No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC10260.11167164617720526No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10090.1098213362502925No Hit
CCACCATACTGCTGACAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC10050.10938596920866599No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10010.10895060216703945No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC9900.10775334280256649No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGTCG9870.10742681752134658No Hit
GTCTATAGCCCCTCCATCAAAGTGATTTTTAAAGAGGCCGACCCACTCCGGCCCCTTCCCTGGAGCCTGGCGGAC9810.10677376695890681No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC9750.106120716396467No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9700.10557650759443382No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9600.1044880899903675No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9540.10383503942792771No Hit
ATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9500.10339967238630118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7600.048.434821
GTATAAT350.001430528538.952871
GTATCAA19650.037.4661181
TTAACGG709.985888E-734.38714235
GTAGTTT1002.6792692E-432.42053270
TATCAAC23650.030.9666842
ATCAACG24700.029.647063
TCAACGC24700.029.5091674
CAACGCA25300.028.8093455
TAGACTC500.006354510328.73324664
AACGCAG25500.028.5865026
TATCGGT605.82964E-428.39540315
CGCTATC605.84079E-428.3861312
GTTTAAC854.611509E-628.26291733
CCGACGC500.00701805428.15479954
CGTGCAA1154.4086846E-827.69223257
ACGCAGA26550.027.584267
AACTGTG20900.027.30150270
GAGTAAT1005.5771307E-727.2670081
GTCTACG500.00820598827.2670081
Produced by FastQC (version 0.11.5)