FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648582
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33780.5208285151299296No Hit
GCCTTGGGCTGACCGAGGACGGTCAATTTGGTCCCGGTGCCGAAAATCAC26030.4013370707173496No Hit
GTCCTGGGCCCAGCCTGCCCTGGCTCAACCTGCCTCCGTGTCTGGGTCCCCTGGGCAGTCGATCACCATCTCCTG23920.3688045613353439No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17570.27089866817148794No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17040.26272699519875664No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA15470.23852034129840174No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14710.22680247062052292No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14710.22680247062052292No Hit
TCTCTGGCCTGCAGACTGACGACGAGGGTGACTATTTCTGCGCCTCATAT14580.22479809800456996No Hit
CCTATGAACACTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14260.21986425771914733No Hit
GGGCACAGGGTCCTGGGCCCAGCCTGCCCTGGCTCAACCTGCCTCCGTGTCTGGGTCCCCTGGGCAGTCGATCAC13840.21338859234453006No Hit
CTCCTGCACTGGAAGCACCAGTGACATTGGTCTTTATGAACATGTCTCCTGGTACCAACAACTCCCAGGCAAAGC13410.20675874446099335No Hit
GAGTATATGAGGCGCAGAAATAGTCACCCTCGTCGTCAGTCTGCAGGCCAGAGATGGTCAGGGAGGCCGTGTTGC12870.19843288897934264No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12390.19103212855120863No Hit
CATATACTCACATCACCACCCTCGTGATTTTCGGCACCGGGACCAAATTGACCGTCCTCGGTCAGCCCAAGGCTG12150.18733174833714164No Hit
CTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTT12100.18656083579254434No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT11790.18178117801604116No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11780.1816269955071217No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG11470.17684733773061848No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11440.1763847902038601No Hit
GGCCAGAGATGGTCAGGGAGGCCGTGTTGCCGGACTTGGAGCCAGAGAAG11330.17468878260574605No Hit
GGCTCAACCTGCCTCCGTGTCTGGGTCCCCTGGGCAGTCGATCACCATCT10870.167596387195451No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10690.16482110203490075No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10570.16297091192786725No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10560.1628167294189478No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10190.15711197658892784No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9660.14894030361619656No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9600.14801520856267983No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG9500.14647338347348524No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9470.14601083594672687No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9140.14092281315238472No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG8870.13675988541155937No Hit
GTCGATCACCATCTCCTGCACTGGAAGCACCAGTGACATTGGTCTTTATGAACATGTCTCCTGGTACCAACAACT8870.13675988541155937No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8850.13645152039372047No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8680.13383041774208965No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC8660.13352205272425074No Hit
CCCCTGGGCAGTCGATCACCATCTCCTGCACTGGAAGCACCAGTGACATT8570.13213441014397562No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8560.13198022763505618No Hit
GGCCTGGGCTCTGCTTTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGCCTGCCCTGGCTCAAC8400.12951330749234485No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAAT8320.1282798474209892No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8210.12658383982287513No Hit
ACCTGCCTCCGTGTCTGGGTCCCCTGGGCAGTCGATCACCATCTCCTGCA8090.12473364971584164No Hit
GACCGAGGACGGTCAATTTGGTCCCGGTGCCGAAAATCACGAGGGTGGTG8090.12473364971584164No Hit
CATCACCACCCTCGTGATTTTCGGCACCGGGACCAAATTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGT8060.12427110218908326No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8030.12380855466232489No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA8000.12334600713556651No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACACTCT7970.12288345960880813No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC7940.12242091208204976No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7720.11902889688582168No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAATTTGGTC7520.11594524670743252No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7490.11548269918067414No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7440.11471178663607685No Hit
ACCATGGCCTGGGCTCTGCTTTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGCCTGCCCTGGCT7230.11147395394876825No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7220.11131977143984878No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7110.10962376384173474No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7020.10823612126145961No Hit
TTCCAGGACAGACTCAAACTTACGGCGGACGACTCAACGACCACTGCCTATTTGGCCCTCTCCAGCCTGAGAC7010.10808193875254016No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC6980.10761939122578179No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTTTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC6930.10684847868118448No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGCCTGCCCTGGCTCAACCTGCCTCCGTGTCTGGGT6910.10654011366334558No Hit
GTACGGGGCTGGGGTCTCAGGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTTTTCCTCACC6880.1060775661365872No Hit
GTGCAGGAGATGGTGATCGACTGCCCAGGGGACCCAGACACGGAGGCAGG6880.1060775661365872No Hit
TCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG6870.10592338362766773No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG6700.10330228097603697No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6690.10314809846711749No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA6690.10314809846711749No Hit
GACCATCTCTGGCCTGCAGACTGACGACGAGGGTGACTATTTCTGCGCCT6600.10176045588684236No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG6600.10176045588684236No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6590.10160627337792291No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCGGACTTGGAGCCAGAGAAGCGAGG6570.101297908360084No Hit
CTCCCAGGCAAAGCCCCCAAACTCGTCATTTCTGCTGTCGATAATCGGCCCTCAGGGGTTTCTCCTCGCTTCT6560.10114372585116455No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6530.10068117832440616No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)