FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648582
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32530.5015557015149974No Hit
GCCTTGGGCTGACCGAGGACGGTCAATTTGGTCCCGGTGCCGAAAATCAC24450.37697623430807514No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23550.3630998085053239No Hit
GTCCTGGGCCCAGCCTGCCCTGGCTCAACCTGCCTCCGTGTCTGGGTCCCCTGGGCAGTCGATCACCATCTCCTG23070.35569904807718994No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17400.26827756551985715No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17320.2670441054485015No Hit
TCTCTGGCCTGCAGACTGACGACGAGGGTGACTATTTCTGCGCCTCATAT14230.21940171019238894No Hit
GGGCACAGGGTCCTGGGCCCAGCCTGCCCTGGCTCAACCTGCCTCCGTGTCTGGGTCCCCTGGGCAGTCGATCAC14100.21739733757643598No Hit
CCTATGAACACTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13640.2103049421661409No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA13350.20583364940747664No Hit
CTCCTGCACTGGAAGCACCAGTGACATTGGTCTTTATGAACATGTCTCCTGGTACCAACAACTCCCAGGCAAAGC13180.20321254675584582No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13090.20182490417557072No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13090.20182490417557072No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12790.19719942890798697No Hit
CTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTT12010.18517319321226924No Hit
GAGTATATGAGGCGCAGAAATAGTCACCCTCGTCGTCAGTCTGCAGGCCAGAGATGGTCAGGGAGGCCGTGTTGC11890.18332300310523572No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11770.18147281299820223No Hit
CATATACTCACATCACCACCCTCGTGATTTTCGGCACCGGGACCAAATTGACCGTCCTCGGTCAGCCCAAGGCTG11370.1753055126414239No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG11060.17052585486492072No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10890.1679047522132899No Hit
GGCTCAACCTGCCTCCGTGTCTGGGTCCCCTGGGCAGTCGATCACCATCT10640.16405018949030348No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10500.16189163436543105No Hit
GGCCAGAGATGGTCAGGGAGGCCGTGTTGCCGGACTTGGAGCCAGAGAAG10420.1606581742940754No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9920.15294904884810248No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9430.145394105911049No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG9200.14184790820590149No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9180.14153954318806258No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9150.1410769956613042No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8880.13691406792047883No Hit
ACCTGCCTCCGTGTCTGGGTCCCCTGGGCAGTCGATCACCATCTCCTGCA8720.1344471477777675No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG8590.13244277516181455No Hit
GTCGATCACCATCTCCTGCACTGGAAGCACCAGTGACATTGGTCTTTATGAACATGTCTCCTGGTACCAACAACT8590.13244277516181455No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8560.13198022763505618No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8560.13198022763505618No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8420.12982167251018376No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC8280.12766311738531133No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8270.1275089348763919No Hit
GGCCTGGGCTCTGCTTTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGCCTGCCCTGGCTCAAC8140.12550456226043893No Hit
CATCACCACCCTCGTGATTTTCGGCACCGGGACCAAATTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGT7850.12103326950177465No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7800.12026235695717735No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7770.11979980943041897No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAAT7660.11810380183230494No Hit
TTCCAGGACAGACTCAAACTTACGGCGGACGACTCAACGACCACTGCCTATTTGGCCCTCTCCAGCCTGAGAC7630.11764125430554656No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC7580.11687034176094926No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7490.11548269918067414No Hit
ACCATGGCCTGGGCTCTGCTTTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGCCTGCCCTGGCT7410.11424923910931849No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7360.1134783265647212No Hit
CCCCTGGGCAGTCGATCACCATCTCCTGCACTGGAAGCACCAGTGACATT7320.11286159652904336No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACACTCT7300.11255323151120444No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGCCTGCCCTGGCTCAACCTGCCTCCGTGTCTGGGT7230.11147395394876825No Hit
GACCATCTCTGGCCTGCAGACTGACGACGAGGGTGACTATTTCTGCGCCT7110.10962376384173474No Hit
GACCGAGGACGGTCAATTTGGTCCCGGTGCCGAAAATCACGAGGGTGGTG7020.10823612126145961No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTTTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC7010.10808193875254016No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6980.10761939122578179No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA6860.10576920111874828No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6820.10515247108307045No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA6750.10407319352063425No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6690.10314809846711749No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAATTTGGTC6690.10314809846711749No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC6540.10083536083332562No Hit
TCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG6520.10052699581548671No Hit
CTCCCAGGCAAAGCCCCCAAACTCGTCATTTCTGCTGTCGATAATCGGCCCTCAGGGGTTTCTCCTCGCTTCT6520.10052699581548671No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGTC200.00798953150.68251421
GGTACGG200.00801148450.647353
GGTATCA4850.041.8064651
GTATCAA12100.039.3792721
TGGTATC1350.037.5165522
GTGGTAT1500.036.0464631
AATGGTA1358.3212035E-735.18634870
ACGTACC400.002881289533.7727113
TATGAGG11300.033.02890870
TATCAAC14650.032.4972762
ATCAACG14700.032.3867383
AACGCAG14850.032.0670176
CAACGCA14900.031.7303035
TCAACGC14900.031.725414
ACGCAGA15700.030.3309067
CTAGACC450.00512872830.02018513
ATACGTA450.00512872830.02018511
AATACGT450.00512872830.02018510
TACGTAC450.005130662630.01787212
CGTGCAA604.3419527E-429.85931457
Produced by FastQC (version 0.11.5)