FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341439
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTCGAGTATTCGG15700.45981859131499325No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13790.4038788773397298No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC12690.37166228813931623No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11980.35086794420086753No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10460.3063504754875688No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT10160.2975641329783651No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTCGACCATTCAGGCTGTCATCCCATG9440.27647691095627625No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT9400.27530539862171577No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG8270.24221017517038185No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC8000.23430246691209852No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG7950.2328380764938979No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC7910.23166656415933737No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC7700.22551612440289479No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7670.22463749015197446No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTCGACCATTCAGG7640.22375885590105404No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC7510.21995144081373247No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG7370.21585114764277072No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT7330.21467963530821024No Hit
GTAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTCGCAGATACA7230.21175085447180902No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT7060.20677192704992692No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG6950.20355026812988558No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC6300.18451319269327757No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC6280.18392743652599733No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC6140.17982714335503558No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG6130.1795342652713955No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA6040.17689836251863436No Hit
GTGTATTACTGTGCGAGAGATTGGGCAGCTCGTCCCCTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC6020.17631260635135412No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC5970.17484821593315353No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCATCATCTCCAGAGACAATGCCAAGAACTCTCTGTATCTG5960.17455533784951338No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA5910.17309094743131276No Hit
GATTATTACCATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG5890.17250519126403252No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT5870.17191943509675228No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG5760.16869777617671092No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGCAAGTAATGCTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCC5660.1657689953403097No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC5650.16547611725666955No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT5610.16430460492210908No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG5580.1634259706711887No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG5560.16284021450390845No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG5550.16254733642026833No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTCGAGTATTCGGCGGAGGGAC5340.15639689666382575No Hit
CCCCTGAGGGCCGCTGATTATTACCATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTA5280.154639628161985No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT5150.1508322130746634No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT5060.14819631032190228No Hit
GCATGGGATGACAGCCTGAATGGTCGAGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG4950.14497465140186094No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA4940.14468177331822082No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA4910.14380313906730047No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC4840.1417529924818196No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG4760.1394099678126986No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT4740.13882421164541836No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA4610.13501679655809676No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT4510.13208801572169554No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT4480.13120938147077515No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA4440.13003786913621465No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC4350.12740196638345358No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC4290.1256446978816128No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC4280.1253518197979727No Hit
GCTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCAT4230.12388742937977208No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG4170.12213016087793134No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA4160.12183728279429122No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC4040.11832274579060974No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT3840.11246518411780729No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTACAGCATTACTT3800.11129367178324678No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC3780.11070791561596655No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC3770.11041503753232641No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG3770.11041503753232641No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC3750.10982928136504617No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG3740.10953640328140604No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATGGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG3720.1089506471141258No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGCAAGTAATGCT3710.10865776903048567No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTCGAC3690.10807201286320543No Hit
GTCGAGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC3630.10631474436136469No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG3630.10631474436136469No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT3580.10485035394316407No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTCGACCATTCAGGC3540.10367884160860359No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC3520.10309308544132333No Hit
GTTTACAGCATTACTTGCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGA3510.10280020735768322No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC3510.10280020735768322No Hit
GTATATGGTAATACTAGTACTACTAATGTATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT3460.10133581693948261No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC3430.10045718268856224No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA3420.10016430460492211No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTTA300.002517388267.828270
CTAGTCA150.002537190767.708994
GCCGGTT350.004636424658.1384670
TGGTATC850.051.7774622
GTATTAT200.00789730750.8264051
GTGGTAT950.049.9347151
AGGTTAA305.976911E-446.50140446
GGTATCA2850.041.6122631
TCTGGAG2650.040.95287770
GTATCAA7150.039.3341871
GCTTGTA350.001471098638.724881
AAGGTTA400.002497848634.76590345
TATCAAC8200.034.6802182
TACGGGT2150.034.66211715
TCATACT400.00264684634.35768538
ATCAACG8400.033.8544963
ATAAAAG400.002845452233.8544963
TCAACGC8700.033.0762334
AACGCAG8850.032.520386
CAACGCA9000.031.9783745
Produced by FastQC (version 0.11.5)