FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1405765
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC51560.3667753856441155No Hit
GCCTTGGGCTGACTCAAGACGGTCAGCTTGGTCCCTCCGCCGAACATGGG46420.33021166411171143No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCTGTGTCTGGGTCCCCTGGACAATCGATCACCATCTC45680.3249476263813653No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG40140.28553847904877416No Hit
GATTACTACTGCAGCACATATCGAAGTGTCCCCATGTTCGGCGGAGGGAC34310.24406639801104738No Hit
TCACTGGACTCCAGGCCGAGGACGAGGCTGATTACTACTGCAGCACATAT32580.2317599314252382No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAAGACGGTCAGCTTGGTCCCTC31750.22585567288985003No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31590.22471750256977518No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCTGTGTCTGGGTCCCCTGGACAATCGATCAC30640.21795961629433083No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29150.20736040518863397No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28890.20551087841851234No Hit
GTATTGGTGGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAA28660.20387475858340476No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA28430.2022386387482972No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27560.19604983763289027No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT27440.19519620989283415No Hit
GTGTTACAGCCACCGGCAGTATTGGTGGTTATAACTATGTCTCCTGGTAC26770.19043012167752077No Hit
GTAGTAATCAGCCTCGTCCTCGGCCTGGAGTCCAGTGATGGTCAGGGAGG25870.18402791362709983No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCGCAGGTAG25780.18338769282205775No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23910.1700853272061831No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23760.16901829253111295No Hit
GCTCAGGCGTCAGGCGCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23400.16645740931094458No Hit
CTTCGATATGTGCTGCAGTAGTAATCAGCCTCGTCCTCGGCCTGGAGTCCAGTGATGGTCAGGGAGGCCGTGTTG22700.16147791416061716No Hit
GTCCAGTGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAT22410.15941498045548153No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22400.15934384481047686No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCGCAGGTAGCTGCTGG22360.15905930223045817No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT22320.15877475965043944No Hit
GACTCAGCCTGCCTCTGTGTCTGGGTCCCCTGGACAATCGATCACCATCT22000.15649841901028977No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21430.15244368724502316No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAAGACGG21300.15151892385996238No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCGCAGGTAGCTG21280.151376652569953No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20730.14746419209469577No Hit
GTGTCTGGGTCCCCTGGACAATCGATCACCATCTCCTGTGTTACAGCCAC20650.14689510693465835No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC19940.14184447613932627No Hit
GCCCTGACTCAGCCTGCCTCTGTGTCTGGGTCCCCTGGACAATCGATCAC19090.13579794631392872No Hit
GGCCTGGGCTCTGCTTTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC19090.13579794631392872No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC18930.1346597759938539No Hit
CAGTAATAGATGGCCGTGTCCTCGACTTTCAGGTCGTCCATTTGCAGGTA18440.1311741293886247No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18400.130889586808606No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18110.12882665310347036No Hit
GTTTGGGGACTTTGCCTGGGTGTTGTTGGTACCAGGAGACATAGTTATAACCACCAATACTGCCGGTGGCTGTAA17860.12704826197835342No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG17490.12441624311318036No Hit
GTCCCCAAACTCATCCTCTTCGATGTCAGTTATCGGCCCTCAGGGGTTTCTAGTCGATTCTCTGGCTCCAAGT17480.12434510746817569No Hit
GTCTTGGAGTTGTCTCTGGAAATGGTGAACCGGCCCTTCACGGAGTCTGA17350.12342034408311488No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT17330.12327807279310554No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17290.12299353021308682No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17020.12107286779796055No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC16680.11865425586780151No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAAGACGGTCAGCTTGGTC16450.11701813603269395No Hit
ACCATGGCCTGGGCTCTGCTTTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT16400.11666245780767057No Hit
GACTTCCACTGCTCAGGCGTCAGGCGCAGGTAGCTGCTGGCCGCGTACTT16250.11559542313260039No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16250.11559542313260039No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16200.11523974490757702No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16080.1143861171675209No Hit
CTCCTGTGTTACAGCCACCGGCAGTATTGGTGGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGT15710.11175409830234784No Hit
GCTTGGTCCCTCCGCCGAACATGGGGACACTTCGATATGTGCTGCAGTAG15640.11125614878731509No Hit
GTAATAGATGGCCGTGTCCTCGACTTTCAGGTCGTCCATTTGCAGGTACA15610.11104274185230105No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15410.10962002895220753No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15380.10940662201719348No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15090.10734368831205784No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCTGTGTCTGGGT15030.10691687444202978No Hit
AGTCTGCCCTGACTCAGCCTGCCTCTGTGTCTGGGTCCCCTGGACAATCGATCACCATCTCCTGTGTTACAGCCA14790.10520961896191754No Hit
GGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGTCCCCAAACTCATCCTCTTCGATGTCAGTTAT14630.10407144864184269No Hit
GCCTGCCTCTGTGTCTGGGTCCCCTGGACAATCGATCACCATCTCCTGTG14530.10336009219179593No Hit
CTCCAAGACCACGCTGTACCTGCAAATGGACGACCTGAAAGTCGAGGACA14440.10271987138675383No Hit
AACTCATCCTCTTCGATGTCAGTTATCGGCCCTCAGGGGTTTCTAGTCGATTCTCTGGCTCCAAGTCTGGCAACA14420.10257760009674448No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14340.10200851493670705No Hit
GACCATCACTGGACTCCAGGCCGAGGACGAGGCTGATTACTACTGCAGCACATATCGAAGTGTCCCCATGTTCGG14320.10186624364669769No Hit
GTATCAACGCAGAGTACGGGGCTGGGGTCTCAGGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCT14320.10186624364669769No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT14230.10122602284165562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)