FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1405765
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC49910.35503800421834375No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44680.3178340618808976No Hit
GCCTTGGGCTGACTCAAGACGGTCAGCTTGGTCCCTCCGCCGAACATGGG44370.3156288568857526No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCTGTGTCTGGGTCCCCTGGACAATCGATCACCATCTC43410.3087998349653036No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41770.29713358918453653No Hit
GATTACTACTGCAGCACATATCGAAGTGTCCCCATGTTCGGCGGAGGGAC31440.22365046789470502No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCTGTGTCTGGGTCCCCTGGACAATCGATCAC31100.22123185596454598No Hit
TCACTGGACTCCAGGCCGAGGACGAGGCTGATTACTACTGCAGCACATAT30470.21675031032925132No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30280.21539873307416246No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAAGACGGTCAGCTTGGTCCCTC29170.20750267647864332No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28580.20330567342336736No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28280.201171604073227No Hit
GTATTGGTGGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAA28200.20060251891318961No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT27640.19661892279292773No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26630.1894342226474553No Hit
GTGTTACAGCCACCGGCAGTATTGGTGGTTATAACTATGTCTCCTGGTAC26450.1881537810373711No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA25970.1847392700771466No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCGCAGGTAG25300.17997318186183323No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAAGACGG24930.17734116299666017No Hit
GTAGTAATCAGCCTCGTCCTCGGCCTGGAGTCCAGTGATGGTCAGGGAGG24270.1726462104263515No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22830.16240267754567797No Hit
GCTCAGGCGTCAGGCGCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22270.15841908142541605No Hit
CTTCGATATGTGCTGCAGTAGTAATCAGCCTCGTCCTCGGCCTGGAGTCCAGTGATGGTCAGGGAGGCCGTGTTG22210.15799226755538798No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCGCAGGTAGCTGCTGG21790.15500457046519156No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21140.15038075353988753No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCGCAGGTAGCTG21100.15009621095986883No Hit
GACTCAGCCTGCCTCTGTGTCTGGGTCCCCTGGACAATCGATCACCATCT20820.14810441289973786No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20800.1479621416097285No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20670.1470373782246677No Hit
GTCCAGTGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAT20480.14568580096957884No Hit
GTGTCTGGGTCCCCTGGACAATCGATCACCATCTCCTGTGTTACAGCCAC19820.14099084839927015No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19490.1386433721141158No Hit
GGCCTGGGCTCTGCTTTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC18930.1346597759938539No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18790.1336638769637884No Hit
GTCCCCAAACTCATCCTCTTCGATGTCAGTTATCGGCCCTCAGGGGTTTCTAGTCGATTCTCTGGCTCCAAGT18410.13096072245361068No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17990.12797302536341423No Hit
GTTTGGGGACTTTGCCTGGGTGTTGTTGGTACCAGGAGACATAGTTATAACCACCAATACTGCCGGTGGCTGTAA17650.12555441343325519No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17640.12548327778825052No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17530.12470078569319908No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC17510.12455851440318973No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17300.1230646658580915No Hit
GCCCTGACTCAGCCTGCCTCTGTGTCTGGGTCCCCTGGACAATCGATCAC17250.12270898763306812No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG16960.12064605392793248No Hit
GTCTTGGAGTTGTCTCTGGAAATGGTGAACCGGCCCTTCACGGAGTCTGA16830.11972129054287169No Hit
CTCCTGTGCTGCCTCTGGAGTAATCTTTGACAACTATGCCATGGCCTGGGTCCGCCAGGCTCCAGGGAAGGGG16660.11851198457779215No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16190.11516860926257233No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT16150.11488406668255363No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC16020.11395930329749283No Hit
CTCCTGTGTTACAGCCACCGGCAGTATTGGTGGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGT15790.11232318346238526No Hit
ACCATGGCCTGGGCTCTGCTTTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT15780.11225204781738057No Hit
CAGTAATAGATGGCCGTGTCCTCGACTTTCAGGTCGTCCATTTGCAGGTA15720.11182523394735251No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15620.11111387749730572No Hit
GACTTCCACTGCTCAGGCGTCAGGCGCAGGTAGCTGCTGGCCGCGTACTT15350.10919321508217947No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAAGACGGTCAGCTTGGTC15270.10862412992214204No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC14890.1059209754119643No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14690.10449826251187075No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTTTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC14490.10307554961177723No Hit
GCCTGCCTCTGTGTCTGGGTCCCCTGGACAATCGATCACCATCTCCTGTG14280.10158170106667899No Hit
GCTTGGTCCCTCCGCCGAACATGGGGACACTTCGATATGTGCTGCAGTAG14270.10151056542167432No Hit
AACTCATCCTCTTCGATGTCAGTTATCGGCCCTCAGGGGTTTCTAGTCGATTCTCTGGCTCCAAGTCTGGCAACA14190.1009414802616369No Hit
GGATGAGTTTGGGGACTTTGCCTGGGTGTTGTTGGTACCAGGAGACATAGTTATAACCACCAATACTGCCGGTGG14190.1009414802616369No Hit
AGTCTGCCCTGACTCAGCCTGCCTCTGTGTCTGGGTCCCCTGGACAATCGATCACCATCTCCTGTGTTACAGCCA14130.10051466639160883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12200.043.2030371
GTATCAA29450.037.0648421
AATGGTA4050.035.9948870
GTGGTAT3400.034.0049671
TATCAAC34000.032.160032
ATCAACG34500.031.3964213
TGGTATC3600.031.1279772
TCAACGC35000.031.044924
CAACGCA35800.030.448195
AACGCAG36200.030.3015146
GACAGGT2900.029.3146291
ACGCAGA38000.028.955557
CGCAGAG38300.028.728748
CAGAGTA39150.028.45197710
AGAGTAC39200.028.32905411
GCAGAGT39850.027.6965339
GAGTTTA4600.027.3518221
TGGATAC500.008309280527.19816424
ACACCGT756.863226E-527.1681696
GTGTGCG500.00835412627.1681697
Produced by FastQC (version 0.11.5)