FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477063
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGAAACA19910.41734529821008964No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8720.18278508289261586No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC8660.1815273873681254No Hit
GTCCTGTGCAGCCTCTGGATTCAGTTTCAGTGACTTCTACATGAGTTGGATCCGCCAGGCTCCAGGGAAGGGA8460.17733506895315712No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA8010.16790235251947858No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7970.1670638888364849No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7960.1668542729157365No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7830.16412926594600713No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT7510.15742155648205794No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCT7330.1536484699085865No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG7160.15008499925586347No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC7050.14777922412763095No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT6980.14631191268239205No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG6750.14149074650517857No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT6690.14023305098068808No Hit
AGCCTGGAGGGTCCCTGAGACTGTCCTGTGCAGCCTCTGGATTCAGTTTC6650.13939458729769444No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT6630.1389753554561976No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT6630.1389753554561976No Hit
GTCTATTACTGTGCGAGAGATGGCCTACGGACCAGAGTGGCTGACCCGTA6610.13855612361470077No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG6590.13813689177320396No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGTTGTACACTGGTACCAGCAGCTTCCAGGAACAGC6570.13771765993170715No Hit
CAGTAATAGACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGAAA6550.1372984280902103No Hit
ATATATGACACTTCCACTATTACTAATATATGCAACCCACTCCAGTCCCTTCCCTGGAGCCTGGCGGATCCAACT6440.13499265296197777No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGTTTCCATTCGGTGAT6190.12975225494326745No Hit
GTCATATATTATGCAGACTCTGTGAAGGGCCGATTCACCATCGCCAGGGACAATGCCAAGAACTCACTGTTTCTG6190.12975225494326745No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG6150.1289137912602738No Hit
CCCTGAGACTGTCCTGTGCAGCCTCTGGATTCAGTTTCAGTGACTTCTAC5950.12472147284530555No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTCGAACCACTG5760.12073877035108571No Hit
GAGTACGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGT5640.11822337930210475No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC5530.11591760417387222No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG5420.11361182904563967No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG5150.10795219918543254No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA5120.10732335142318729No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT5110.10711373550243888No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG5100.10690411958169048No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG5050.10585603997794842No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT5020.10522719221570317No Hit
GTTGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT4970.10417911261196111No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG4940.10355026484971587No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC4910.10292141708747062No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC4880.10229256932522539No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA4850.10166372156298015No Hit
CTCCAGTCCCTTCCCTGGAGCCTGGCGGATCCAACTCATGTAGAAGTCAC4830.10124448972148331No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4790.10040602603848968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1250.047.06347741
AGGGGGG2300.045.97899670
AGACGCT1500.045.68125
TTATACA1350.045.6618462
TAGCACA306.54465E-445.657064
TATACCA451.9574036E-645.6570555
GACGCTC1450.044.898126
ATGCCGT1350.043.7208544
TATACAC1500.043.3742073
CGAGACG1450.042.5172123
CACGAGA1450.042.51275321
GAGACGC1550.041.98383724
CTTATAC1500.041.125841
CCGTCTT1550.040.45747
ATCTCCG1550.039.76582710
ACACATC1550.039.7658276
TCAAGCG955.4897682E-639.2885570
GTATGCC1500.039.26531242
ATCTCGT1250.038.5946437
GGTATCA8050.037.464531
Produced by FastQC (version 0.11.5)