FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477063
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGAAACA18280.3831779031280984No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14540.3047815487681921No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8730.18299469881336428No Hit
GTCCTGTGCAGCCTCTGGATTCAGTTTCAGTGACTTCTACATGAGTTGGATCCGCCAGGCTCCAGGGAAGGGA8380.17565814158716983No Hit
GTACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT8270.17335236645893728No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC7800.1635004181837619No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7310.15322923806708966No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG7110.1490369196521214No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT7110.1490369196521214No Hit
GTCTATTACTGTGCGAGAGATGGCCTACGGACCAGAGTGGCTGACCCGTA7090.1486176878106246No Hit
ATATATGACACTTCCACTATTACTAATATATGCAACCCACTCCAGTCCCTTCCCTGGAGCCTGGCGGATCCAACT6510.13645996440721667No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG6490.13604073256571983No Hit
GTCATATATTATGCAGACTCTGTGAAGGGCCGATTCACCATCGCCAGGGACAATGCCAAGAACTCACTGTTTCTG6360.13331572559599048No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGTTTCCATTCGGTGAT6220.13038110270551267No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA6210.13017148678476428No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGTTGTACACTGGTACCAGCAGCTTCCAGGAACAGC6180.12954263902251903No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG6000.1257695524490476No Hit
AGCCTGGAGGGTCCCTGAGACTGTCCTGTGCAGCCTCTGGATTCAGTTTC5910.1238830091623119No Hit
CTGTACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC5820.12199646587557618No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC5800.12157723403407934No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG5760.12073877035108571No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT5760.12073877035108571No Hit
CAGTAATAGACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGAAA5730.12010992258884047No Hit
CCCTGAGACTGTCCTGTGCAGCCTCTGGATTCAGTTTCAGTGACTTCTAC5710.11969069074734365No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT5700.11948107482659524No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCT5510.11549837233237538No Hit
CTCCAGTCCCTTCCCTGGAGCCTGGCGGATCCAACTCATGTAGAAGTCAC5380.11277336536264601No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5320.11151566983815554No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG5160.10816181510618095No Hit
GAGTACGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGT5120.10732335142318729No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC5110.10711373550243888No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG4930.10334064892896745No Hit
TCACTGGACTCCAGGCTGAGGATGAGGCTGATTATTATTGCCAGTCCTAT4890.1025021852459738No Hit
ACTCTGGTCCGTAGGCCATCTCTCGCACAGTAATAGACGGCCGTGTCCTC4790.10040602603848968No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATCG350.006134049554.17622870
GGTCTAG200.00742161351.6345736
CCCGTAA200.007599632751.3259662
GGTATCA7400.043.5108571
GACAGGT759.331416E-1041.1038741
GTATCAA19500.041.103871
ATCCGTT501.9031049E-434.36504732
TATCAAC24950.032.0915872
GTGGTAT1950.031.6183661
TGGTATC1950.031.585212
TTGTCCC6650.031.2630865
TCAACGC25800.031.1538664
TTGGCAT6700.031.14062159
AACGCAG25850.031.0968656
ATCAACG26100.030.7957743
GCATTGT6600.030.7105762
TGGCATT6600.030.58724660
ACGCAGA26400.030.4490157
CAACGCA26450.030.3882685
CGCAGAG26600.030.3486718
Produced by FastQC (version 0.11.5)