FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433412
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT18760.4328444989986433No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9830.22680498001901192No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9270.2138842487056196No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8600.1984255165985252No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7950.1834282391811948No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7400.17073823521268447No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7160.16520077893551632No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7000.16150914141740422No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6630.15297222965677No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT6070.14005149834337768No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG5540.12782294906463135No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG5520.12736149437486732No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG5410.12482349358116528No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5280.12182403809769919No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC4830.11144130757800891No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG4740.10936476147407086No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC4680.10798039740477883No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4610.10636530599060479No Hit
CTCCTAAACTCCTCCTTTACTGGGCATCTTCCCGGCACGCCGGGGTCCCT4490.10359657785202071No Hit
GTTTATTACTGTCAGCAATATCTTGTTACTCCTCCGACGTTCGGCCAAGGGACCCAGGTGGAAATCAAACGAA4390.10128930440320065No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG4350.10036639502367262No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAT150.002662212666.895511
GGCTAAC200.00831410350.171631
CGGTATC200.00833689350.13692
TGGTATC3050.048.2190932
GTGGTAT3250.044.2539521
GGTATCA11200.043.601541
TTATACA3650.043.0398942
CTTATAC3900.040.3088341
TATACAC3900.040.280933
TCGTATG4150.039.1835440
GCCGTCT4250.038.87442846
CGTATGC4200.038.8097741
GTATGCC4250.038.43140442
TGCTTGA4600.037.75274755
TATTCTA851.09329136E-437.7506670
TACACAT4250.037.7501375
CCGTCTT4500.037.57341447
CTTGAAA4800.037.14686657
CTCGTAT4300.036.86676439
TCTTATA1001.2732926E-1136.7670562
Produced by FastQC (version 0.11.5)