FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444762
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16820.3781797905396594No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15280.3435545302881092No Hit
GTATAGCTCTAATTCTCCGTACACTTTTGGCCAGGGGACCAAAGTGGAGATCAAACGAACTGTGGCTGCACCAT13590.305556679752317No Hit
GAGTATCAGTTACTGGTTGGCCTGGTATCAGCTGAAACCAGGGAAAGCCCCTAAGTTGTTGATCTATAAGGCGT13290.29881149918383315No Hit
CCTTTCACCCTGTCTGCATCTGTAGGAGACACAGTCACCATCACTTGCCG13130.29521406954730844No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12240.2752033671941398No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT11750.2641862389322829No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10700.24057810694258952No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10530.23675583795378202No Hit
GTCTGCATCTGTAGGAGACACAGTCACCATCACTTGCCGGGCCAGTCAGA10380.2333832476695401No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10240.230235496737581No Hit
AGCCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9770.2196680471802897No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9590.2156209388391994No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9170.20617768604332204No Hit
GAATTCACTCTCACCATCAGCGGCCTGCAGCCTGATGATTTTGCAACTTA8940.2010063809408178No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8420.1893147346221125No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8350.18774085915613292No Hit
CTTTTGGCCAGGGGACCAAAGTGGAGATCAAACGAACTGTGGCTGCACCA8320.18706634109928458No Hit
GAGCTATACTGTTGACAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCCGCTGATGGTGAGAGTGAATTCTGTC8180.18391859016732545No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8010.18009632117851795No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7650.17200210449633738No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7200.16188433364361163No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7150.16076013688219765No Hit
TCTATAAGGCGTCTAATTTAGAAACTGGAGTTCCATCGAGGTTCACCGGCAGTGGATTTGGGACAGAATTCACT6920.1555888317796934No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6860.15423979566599666No Hit
GTAGGAGACACAGTCACCATCACTTGCCGGGCCAGTCAGAGTATCAGTTACTGGTTGGCCTGGTATCAGCTGAAA6800.1528907595522999No Hit
GGCCAAAAGTGTACGGAGAATTAGAGCTATACTGTTGACAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCCG6610.1486188118589268No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCCCCTGGCCAAAAGTG6580.1479442938020784No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6310.14187363129044298No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6250.14052459517674623No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT6230.14007491647218062No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6120.1376016835970699No Hit
ATACTGTTGACAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCCGCTGA6110.13737684424478708No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6100.13715200489250431No Hit
GAATTAGAGCTATACTGTTGACAGTAATAAGTTGCAAAATCATCAGGCTG6020.13535329007424196No Hit
GTCTCTGGTGGCTCCATCAGTAATTTCTACTGGAGCTGGATCCGCCAGCC6010.13512845072195914No Hit
GTAATATAGGGCCGTGTCCGCGGCGGTCACAGAGTTCAGGCTCAGGGAGAACTGGTTCTTGGACGTGTCTATTGA5830.13108134238086888No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCC5800.13040682432402048No Hit
ACTTATTACTGTCAACAGTATAGCTCTAATTCTCCGTACACTTTTGGCCA5750.1292826275626065No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTTCACC5710.12838327015347534No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5630.126584555335213No Hit
GACCCAGCCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG5490.12343680440325387No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5370.12073873217586036No Hit
GGTATCAGCTGAAACCAGGGAAAGCCCCTAAGTTGTTGATCTATAAGGCGTCTAATTTAGAAACTGGAGTTCCAT5320.11961453541444637No Hit
GAGTGAATTCTGTCCCAAATCCACTGCCGGTGAACCTCGATGGAACTCCA5280.11871517800531521No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5110.11489290901650771No Hit
CAACAGTATAGCTCTAATTCTCCGTACACTTTTGGCCAGGGGACCAAAGTGGAGATCAAACGAACTGTGGCTGCA5050.11354387290281096No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG4860.10927192520943786No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT4820.10837256780030669No Hit
CACCATCAGCGGCCTGCAGCCTGATGATTTTGCAACTTATTACTGTCAACAGTATAGCTCTAATTCTCCGTACA4780.1074732103911755No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4660.10477513816378198No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC4650.10455029881149917No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA4630.1041006201069336No Hit
CCTTATAGATCAACAACTTAGGGGCTTTCCCTGGTTTCAGCTGATACCAGGCCAACCAGTAACTGATACTCTGA4630.1041006201069336No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG4630.1041006201069336No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA4590.10320126269780243No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT4580.10297642334551962No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA4550.10230190528867124No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT4520.10162738723182285No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA4520.10162738723182285No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG4500.10117770852725728No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTC200.006377725453.6512459
ACTCTCG200.006675554453.0357954
GGCTTAC200.007810427350.9713971
ACGCGTA2300.039.9625927
CGTATGC2350.039.78945541
GACGCGT2400.039.6980126
CGTAGTA2350.039.218430
GCGTAGT2350.039.1697129
ATGCCGT2500.038.93914444
TCGTATG2400.038.89362340
GAGTTTA1400.038.835351
CCGTCTT2550.038.36107647
GTATCAA12000.037.66221
GTGGTAT1450.037.49621
CTTATAC2550.037.3123971
GTATGCC2550.036.7276742
CACGAGA2500.036.68702321
CGAGACG2600.036.5948523
ATCTCCG2600.036.57016810
AATCTCG2350.036.5321436
Produced by FastQC (version 0.11.5)