FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444762
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21790.489924948624208No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16560.3723339673803068No Hit
GTATAGCTCTAATTCTCCGTACACTTTTGGCCAGGGGACCAAAGTGGAGATCAAACGAACTGTGGCTGCACCAT12810.28801921027425903No Hit
GAGTATCAGTTACTGGTTGGCCTGGTATCAGCTGAAACCAGGGAAAGCCCCTAAGTTGTTGATCTATAAGGCGT12650.2844217806377343No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12010.2700320620916355No Hit
CCTTTCACCCTGTCTGCATCTGTAGGAGACACAGTCACCATCACTTGCCG11800.26531043569369683No Hit
GTCTGCATCTGTAGGAGACACAGTCACCATCACTTGCCGGGCCAGTCAGA10700.24057810694258952No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10200.22933613932844982No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9870.2219164407031176No Hit
AGCCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9740.2189935291234413No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9700.21809417171431014No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9330.20977511567984675No Hit
GAATTCACTCTCACCATCAGCGGCCTGCAGCCTGATGATTTTGCAACTTA9240.20775156150930157No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8570.1926873249063544No Hit
GAGCTATACTGTTGACAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCCGCTGATGGTGAGAGTGAATTCTGTC7890.1773982489511244No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7880.17717340959884162No Hit
CTTTTGGCCAGGGGACCAAAGTGGAGATCAAACGAACTGTGGCTGCACCA7630.17155242579177177No Hit
TCTATAAGGCGTCTAATTTAGAAACTGGAGTTCCATCGAGGTTCACCGGCAGTGGATTTGGGACAGAATTCACT7550.16975371097350944No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7370.16570660263241913No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG7360.16548176328013633No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7240.16278369105274282No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7190.16165949429132886No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7090.1594111007685009No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7080.1591862614162181No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6810.15311559890458268No Hit
GAATTAGAGCTATACTGTTGACAGTAATAAGTTGCAAAATCATCAGGCTG6420.1443468641655537No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6400.14389718546098812No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6270.14097427388131178No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6150.1382762016539183No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCCCCTGGCCAAAAGTG6080.1367023261879387No Hit
GGCCAAAAGTGTACGGAGAATTAGAGCTATACTGTTGACAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCCG6080.1367023261879387No Hit
ATACTGTTGACAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCCGCTGA5950.1337794146082624No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA5930.13332973590369682No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT5870.13198069979000004No Hit
ACTTATTACTGTCAACAGTATAGCTCTAATTCTCCGTACACTTTTGGCCA5670.12748391274434417No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT5640.12680939468749577No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5560.12501067986923342No Hit
GAGTGAATTCTGTCCCAAATCCACTGCCGGTGAACCTCGATGGAACTCCA5550.12478584051695063No Hit
GTAATATAGGGCCGTGTCCGCGGCGGTCACAGAGTTCAGGCTCAGGGAGAACTGGTTCTTGGACGTGTCTATTGA5420.12186292893727431No Hit
GTAGGAGACACAGTCACCATCACTTGCCGGGCCAGTCAGAGTATCAGTTACTGGTTGGCCTGGTATCAGCTGAAA5390.12118841088042594No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5270.11849033865303239No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTTCACC5240.11781582059618403No Hit
GTCTCTGGTGGCTCCATCAGTAATTTCTACTGGAGCTGGATCCGCCAGCC5070.11399355160737652No Hit
GACCCAGCCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG5030.11309419419824535No Hit
CAACAGTATAGCTCTAATTCTCCGTACACTTTTGGCCAGGGGACCAAAGTGGAGATCAAACGAACTGTGGCTGCA5020.11286935484596257No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG4830.10859740715258948No Hit
GGTATCAGCTGAAACCAGGGAAAGCCCCTAAGTTGTTGATCTATAAGGCGTCTAATTTAGAAACTGGAGTTCCAT4750.10679869233432711No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT4720.10612417427747875No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA4640.10432545945921638No Hit
CACCATCAGCGGCCTGCAGCCTGATGATTTTGCAACTTATTACTGTCAACAGTATAGCTCTAATTCTCCGTACA4600.10342610205008522No Hit
CCTTATAGATCAACAACTTAGGGGCTTTCCCTGGTTTCAGCTGATACCAGGCCAACCAGTAACTGATACTCTGA4570.10275158399323683No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCCGCTGATGGTGAGAGTGA4470.1005031904704089No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG4470.1005031904704089No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)