FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957511
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT33660.35153643143525243No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC31830.3324243794588261No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA31250.32636700779416633No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCCGCGTCTGCTGGACAGTCGATCACCATCTCCTG30300.3164454507572237No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC28900.30182420880804506No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28090.29336477596602023No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC28040.29284258875354957No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC27180.2838609686990541No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG27070.28271215683161866No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC25580.26715097789999276No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAGAGTGGTG24870.25973591948290936No Hit
GATTATTACTGCAGCTCATATGCAGGCAGCACCACTCTTTATGTCTTCGG24870.25973591948290936No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAGAGTGGTGC21630.22589818811481016No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC21250.2219295653000331No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCCGCGTCTGCTGGACAGTCGATCAC20540.21451450688294965No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20340.21242575803306699No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACTATGTCTCCTGGTACCAACAGCACCCAGACAAGGC19410.20271307588111256No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAGAGTGGTGCTGCCTGCATATGAG19310.20166870145617127No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACTATGTCTCCTGGTACCAACAGCA19270.20125095168619472No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC19070.19916220283631206No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT18500.19320926861414647No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18390.192060456746711No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG18360.1917471444192286No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG18050.18850958370191045No Hit
CTCTTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCC17550.1832877115772038No Hit
GTAATAATCAGCCTCGTCCTCAATTTGGAGCCCAGAGATGGTCAGGGAGG17550.1832877115772038No Hit
TCTCTGGGCTCCAAATTGAGGACGAGGCTGATTATTACTGCAGCTCATAT16620.1735750294252494No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG16570.17305284221277875No Hit
GACTCAGCCTGCCTCCGTGTCCGCGTCTGCTGGACAGTCGATCACCATCT15910.16615997100816596No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG15890.1659510961231777No Hit
GTAATAGAAGGCGGTGTCCTCAGGTCTCAGACTGTTCATTTGTAGATAAA15550.16240022307837718No Hit
GCTTATAACTATGTCTCCTGGTACCAACAGCACCCAGACAAGGCCCCCAAACTGTTGATTTATGATGTCAATAAT15340.16020703678600037No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC15000.15665616374119984No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT14980.15644728885621156No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA14700.15352304046637585No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG14630.15279197836891692No Hit
CTGCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT14560.15206091627145796No Hit
CATAAAGAGTGGTGCTGCCTGCATATGAGCTGCAGTAATAATCAGCCTCG13300.1389017985171972No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC13230.13817073641973826No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC13180.1376485492072676No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG13020.13597755012736146No Hit
CTTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC12920.1349331757024201No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCCGCGTCTGCTGGACAGTCGATCAC12850.1342021136049612No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT12770.13336661406500813No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12650.13211336475507854No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA12190.1273092424003484No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC11550.12062524608072388No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG11470.1197897465407708No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG11130.11623887349597029No Hit
GACCATCTCTGGGCTCCAAATTGAGGACGAGGCTGATTATTACTGCAGCT11060.11550781139851136No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC11040.11529893651352308No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT10930.1141501246460876No Hit
GACATAAAGAGTGGTGCTGCCTGCATATGAGCTGCAGTAATAATCAGCCT10930.1141501246460876No Hit
GTCTGAGACCTGAGGACACCGCCTTCTATTACTGTGCAAAAGATTATTGTCGTGGTGGTGACTGCTACGGACATA10880.11362793743361695No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10860.11341906254862869No Hit
CTATACAGACTCTGTGAAGGGTCGATTCACCATCTCCAGAGACAACAGCAAAGACTCCCTTTATCTACAAATGAA10860.11341906254862869No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10840.11321018766364042No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10840.11321018766364042No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT10800.11279243789366387No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG10730.11206137579620495No Hit
GTCACATCCTGCCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10690.1116436260262284No Hit
GCCTGCCTCCGTGTCCGCGTCTGCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTG10600.11070368904378122No Hit
GAGATGGTCAGGGAGGCCGTGTCGCCAGACTTGGAGCCAGAGAAGCGATA10370.10830162786641616No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC10280.10736169088396896No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC10250.10704837855648656No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCCGCGTCTGCTGGACAGTCG10240.10694394111399244No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG10210.10663062878651001No Hit
GGTACCAACAGCACCCAGACAAGGCCCCCAAACTGTTGATTTATGATGTC10200.10652619134401589No Hit
GTACCAACAGCACCCAGACAAGGCCCCCAAACTGTTGATTTATGATGTCA9980.10422856760914495No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC9860.10297531829921536No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT9830.10266200597173296No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9830.10266200597173296No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC9790.10224425620175644No Hit
TTCTATTACTGTGCAAAAGATTATTGTCGTGGTGGTGACTGCTACGGACATACTGGGAGTTTTGACTACTGGGGC9760.10193094387427402No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAC150.002479346768.1134810
AGGGGGG2900.068.10068570
ATCGTAT309.248512E-657.36944239
TATCGTA309.3007075E-657.31498738
GAACCGT200.00774144151.090456
ATATCGT352.3315128E-549.0727937
GGTATCA8950.047.582681
GTGGTAT2250.045.4255941
AATATCG405.1659677E-542.8889936
TGGTATC2450.040.312062
CGTATGC2900.039.26482441
TCGTATG2900.039.21709440
GTATAGA350.001433566838.9362221
TATACAC3000.038.597643
GTATCAA23700.037.519241
AGGCAAT3200.036.32186532
ATGCCGT3250.036.25531844
CACGAGA3200.036.18528721
GTATGCC3250.036.15923342
TCTCCGA3300.035.08876411
Produced by FastQC (version 0.11.5)