FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957511
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC32650.34098824974334496No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32280.33712406437106207No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC30920.32292057219185993No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT30480.31832532472211805No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCCGCGTCTGCTGGACAGTCGATCACCATCTCCTG28990.3027641457904922No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA28930.30213752113552744No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC27770.29002277780620794No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25070.261824668332792No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG25050.2616157934478037No Hit
GATTATTACTGCAGCTCATATGCAGGCAGCACCACTCTTTATGTCTTCGG24940.2604669815803683No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC23670.24720342638361337No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC23520.24563686474620136No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAGAGTGGTG21570.2252715634598454No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCCGCGTCTGCTGGACAGTCGATCAC21330.22276506483998618No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC21060.21994525389264458No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAGAGTGGTGC20240.21138138360812567No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG19950.2083526977757958No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19690.20563732427094833No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAGAGTGGTGCTGCCTGCATATGAG19100.19947551516379447No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACTATGTCTCCTGGTACCAACAGCACCCAGACAAGGC18770.19602907956148807No Hit
CTCTTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCC18410.19226933163169926No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC17980.18777852160445155No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACTATGTCTCCTGGTACCAACAGCA17950.18746520927696914No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT17760.1854808978695806No Hit
GTAATAATCAGCCTCGTCCTCAATTTGGAGCCCAGAGATGGTCAGGGAGG17310.18078121295734462No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17280.18046790062986223No Hit
TCTCTGGGCTCCAAATTGAGGACGAGGCTGATTATTACTGCAGCTCATAT16730.1747238412926849No Hit
GACTCAGCCTGCCTCCGTGTCCGCGTCTGCTGGACAGTCGATCACCATCT15810.16511559658322464No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG15600.16292241029084784No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG15490.16177359842341235No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC14950.15613397652872918No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG14750.1540452276788465No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG14450.15091210440402253No Hit
GCTTATAACTATGTCTCCTGGTACCAACAGCACCCAGACAAGGCCCCCAAACTGTTGATTTATGATGTCAATAAT14230.1486144806691516No Hit
CTGCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT14220.14851004322665745No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA13810.14422810808439798No Hit
GTAATAGAAGGCGGTGTCCTCAGGTCTCAGACTGTTCATTTGTAGATAAA13180.1376485492072676No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT12990.13566423779987907No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCCGCGTCTGCTGGACAGTCGATCAC12980.13555980035738494No Hit
CTTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC12690.13253111452505506No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12670.13232223964006679No Hit
CATAAAGAGTGGTGCTGCCTGCATATGAGCTGCAGTAATAATCAGCCTCG12570.13127786521512547No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC12350.12898024148025455No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12040.1257426807629364No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC11900.12428055656801855No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA11460.11968530909827668No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT11410.11916312188580602No Hit
CTATACAGACTCTGTGAAGGGTCGATTCACCATCTCCAGAGACAACAGCAAAGACTCCCTTTATCTACAAATGAA11370.11874537211582947No Hit
GTCTGAGACCTGAGGACACCGCCTTCTATTACTGTGCAAAAGATTATTGTCGTGGTGGTGACTGCTACGGACATA11280.11780543513338226No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG11070.11561224884100549No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC10980.11467231185855828No Hit
GACCATCTCTGGGCTCCAAATTGAGGACGAGGCTGATTATTACTGCAGCT10900.11383681231860521No Hit
GACATAAAGAGTGGTGCTGCCTGCATATGAGCTGCAGTAATAATCAGCCT10900.11383681231860521No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10850.11331462510613456No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG10830.1131057502211463No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG10800.11279243789366387No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC10660.11133031369874602No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10630.11101700137126362No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC10630.11101700137126362No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT10600.11070368904378122No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT10430.10892825252138096No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10170.10621287901653349No Hit
GCCTGCCTCCGTGTCCGCGTCTGCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTG10020.1046463173791215No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCCGCGTCTGCTGGACAGTCG9900.1033930680691919No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC9800.10234869364425056No Hit
GTTATAAGCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCG9790.10224425620175644No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA9610.10036438223686203No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9600.1002599447943679No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)