FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805689
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGACTGGCCTCCGGGAACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT31000.3847638480853034No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCGCATAGTTCAGTCTCAGGGAGA30050.37297269790204407No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28920.358947435052483No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28450.3531139186460284No Hit
GTCTCTGGGGGCTCCATAAGCAGTCGTGATTACTTCTGGAATTGGCTCCG21740.2698311631411128No Hit
GTTAGGACCCAGACGGAACCATGGCAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATTA21740.2698311631411128No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT21690.26921057628936224No Hit
GTCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCGGGCTGCAGGCTGCTGACGGTGAGAGTGAACT18810.2334647736285341No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18210.22601773140752823No Hit
GTGTATTACTGTGCGACCGGGCCGACTGATATCCGTCACTCGGGCCTCGT17250.21410246385391882No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16730.20764836059571373No Hit
GAGTTCACTCTCACCGTCAGCAGCCTGCAGCCCGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATGACTGG15650.19424368459790317No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15590.19349898037580257No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15570.1932507456351024No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15370.19076839822810043No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15270.18952722452459944No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14970.18580370341409652No Hit
GTCTCCAGACACCCTGTCTGTGTCTGTAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAAAGTGTTAGGAG14890.1848107644512957No Hit
CTGATATCCCAGTCAGATTCAGTGGCAGCGGGTCTGGGACAGAGTTCACT14830.18406606022919514No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14350.17810842645239044No Hit
CTCCTGCAGGGCCAGTCAAAGTGTTAGGAGCAGCTTAGCCTGGTACCAACAGAAAAGTGGCCAGGCTCCCAGGCT13780.17103373634243488No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13660.16954432789823368No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13330.16544845467668046No Hit
GTGTTAGGAGCAGCTTAGCCTGGTACCAACAGAAAAGTGGCCAGGCTCCCAGGCTCCTCATCTATGATACATCCA13260.16457963308422977No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC12900.16011140775162624No Hit
GAACCATGGCAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC12280.15241613078992017No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12020.14918907916081764No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11920.14794790545731668No Hit
GTCTGTGTCTGTAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAAAGTGTTAGGAGCAGCTTAGCCTGGTA11870.14732731860556617No Hit
GTTTATTACTGTCAGCAGTATAATGACTGGCCTCCGGGAACGTTCGGCCA11660.1447208538282141No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11550.14335556275436304No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11330.1406249806066609No Hit
GCTTAGCCTGGTACCAACAGAAAAGTGGCCAGGCTCCCAGGCTCCTCATC11040.13702557686650804No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGCAGCCCCAGCGCAGCTTCTCT10860.13479146420020627No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10760.1335502904967053No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10750.1334261731263552No Hit
GTATTACTGTGCGACCGGGCCGACTGATATCCGTCACTCGGGCCTCGTCAGCTATTGGGGCCAGGGAACCCTGGT10570.13119206046005344No Hit
CTCCAGACACCCTGTCTGTGTCTGTAGGGGAAAGAGCCACCCTCTCCTGC10570.13119206046005344No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10550.13094382571935326No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10540.13081970834900317No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10240.1270961872385002No Hit
CCATATTACTTGACACGTCTCAGAAGCAGTTCTCCCTGAGACTGAACTAT10080.12511030931289865No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10030.12448972246114816No Hit
CAGTAATACACGGCCGTGTCCGCGGCAGTCGCATAGTTCAGTCTCAGGGA10010.12424148772044796No Hit
CACCTGCACTGTCTCTGGGGGCTCCATAAGCAGTCGTGATTACTTCTGGA10000.12411737035009787No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9930.12324854875764718No Hit
CCCTGTCTGTGTCTGTAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAAAGTGTTAGGAGCAGCTTAGC9910.12300031401694698No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9850.12225560979484641No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9490.11778738446224288No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9450.11729091498084247No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9450.11729091498084247No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9440.11716679761049238No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTTCCCGGAGGCCAGTCATTATACTG9420.1169185628697922No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC9190.11406386335173993No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9030.11207798542613838No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8810.10934740327843621No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8780.10897505116738593No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8730.10835446431563545No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT8720.10823034694528536No Hit
GTCAAAGTGTTAGGAGCAGCTTAGCCTGGTACCAACAGAAAAGTGGCCAGGCTCCCAGGCTCCTCATCTATGATA8690.10785799483423505No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG8210.10190036105743035No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8180.10152800894638006No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC8120.10078330472427946No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8100.10053506998357928No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT150.002355437568.998839
CTACGCT4400.051.77424628
TCGCGTA200.007432507451.6186438
CCGTATG200.00774281751.0873930
ACTACGC4500.050.598527
TCGTATG3600.049.93974740
CGTATGC3750.049.8650241
GTGGTAT2250.049.7195171
TCTCCGA4600.049.3633511
TACGCTG4650.049.0395429
CGAGACT4650.048.1335923
ACGAGAC4700.047.61857622
TGGTATC2350.047.5860672
ATGCCGT4100.047.53863544
TACACAT4950.047.2423555
GTATGCC4050.047.11298842
CCCACGA4850.046.82756819
CACGAGA4800.046.6207321
CGTATTC306.195701E-446.17131441
CTTATAC5000.046.103551
Produced by FastQC (version 0.11.5)