FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805689
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47570.5904263307554156No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29060.3606850782373844No Hit
GTATAATGACTGGCCTCCGGGAACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT28620.35522391394198005No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCGCATAGTTCAGTCTCAGGGAGA27310.3389645384261173No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24470.3037152052466895No Hit
GTCTCTGGGGGCTCCATAAGCAGTCGTGATTACTTCTGGAATTGGCTCCG22620.2807534917319214No Hit
GTCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCGGGCTGCAGGCTGCTGACGGTGAGAGTGAACT20370.25282708340314936No Hit
GTTAGGACCCAGACGGAACCATGGCAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATTA18920.23483006470238518No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18100.22465244033367712No Hit
GTGTATTACTGTGCGACCGGGCCGACTGATATCCGTCACTCGGGCCTCGT16990.21087541222481626No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15670.19449191933860335No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15360.19064428085775034No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15190.18853428556179866No Hit
GAGTTCACTCTCACCGTCAGCAGCCTGCAGCCCGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATGACTGG14470.17959783489659162No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14140.1755019616750384No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13160.1633384593807288No Hit
CTGATATCCCAGTCAGATTCAGTGGCAGCGGGTCTGGGACAGAGTTCACT13040.1618490509365276No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13030.1617249335661775No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC12680.15738082560392408No Hit
CTCCTGCAGGGCCAGTCAAAGTGTTAGGAGCAGCTTAGCCTGGTACCAACAGAAAAGTGGCCAGGCTCCCAGGCT12450.15452612608587182No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11960.14844437493871704No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11910.14782378808696656No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11760.14596202753171508No Hit
GTCTCCAGACACCCTGTCTGTGTCTGTAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAAAGTGTTAGGAG11700.1452173233096145No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11320.14050086323631078No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11300.1402526284956106No Hit
GTTTATTACTGTCAGCAGTATAATGACTGGCCTCCGGGAACGTTCGGCCA11170.1386391026810593No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11130.13814263319965894No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11100.13777028108860864No Hit
CACCTGCACTGTCTCTGGGGGCTCCATAAGCAGTCGTGATTACTTCTGGA11070.13739792897755834No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11000.13652910738510765No Hit
GAACCATGGCAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC10630.131936764682154No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10550.13094382571935326No Hit
GTCCCAGACCCGCTGCCACTGAATCTGACTGGGATATCAGTGGCCCTGGT10490.13019912149725268No Hit
GTGTTAGGAGCAGCTTAGCCTGGTACCAACAGAAAAGTGGCCAGGCTCCCAGGCTCCTCATCTATGATACATCCA10420.12933029990480197No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10200.12659971775709983No Hit
GTATTACTGTGCGACCGGGCCGACTGATATCCGTCACTCGGGCCTCGTCAGCTATTGGGGCCAGGGAACCCTGGT10170.12622736564604953No Hit
GTCTGTGTCTGTAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAAAGTGTTAGGAGCAGCTTAGCCTGGTA10100.12535854405359886No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9970.12374501823904757No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTTCCCGGAGGCCAGTCATTATACTG9650.11977326238784443No Hit
GCTTAGCCTGGTACCAACAGAAAAGTGGCCAGGCTCCCAGGCTCCTCATC9520.11815973657329318No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9510.11803561920294307No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9350.1160497412773415No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC9180.11393974598138984No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9110.11307092438893915No Hit
CCATATTACTTGACACGTCTCAGAAGCAGTTCTCCCTGAGACTGAACTAT9010.11182975068543817No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGCAGCCCCAGCGCAGCTTCTCT8950.11108504646333758No Hit
GAGTGAACTCTGTCCCAGACCCGCTGCCACTGAATCTGACTGGGATATCA8920.11071269435228731No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8820.10947152064878633No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8780.10897505116738593No Hit
CCCTGTCTGTGTCTGTAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAAAGTGTTAGGAGCAGCTTAGC8730.10835446431563545No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8720.10823034694528536No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8590.10661682113073406No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8550.10612035164933367No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8430.10463094320513251No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8220.10202447842778044No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8200.10177624368708026No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8150.10115565683532976No Hit
GTAATAAACTGCAAAATCTTCGGGCTGCAGGCTGCTGACGGTGAGAGTGAACTCTGTCCCAGACCCGCTGCCA8110.10065918735392937No Hit
CTCCAGACACCCTGTCTGTGTCTGTAGGGGAAAGAGCCACCCTCTCCTGC8090.10041095261322919No Hit
CAGTAATACACGGCCGTGTCCGCGGCAGTCGCATAGTTCAGTCTCAGGGA8080.10028683524287907No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8060.10003860050217887No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8060.10003860050217887No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCTCG200.00794145950.761087
AATGGTA2050.042.4665470
GTGGTAT2650.042.167451
GTATCAA20650.039.5190281
GGTATCA7400.039.3528331
TGGTATC2700.038.851752
GTGATAC350.001477109938.699131
TAGATGG350.001482076438.672715
TATCAAC22650.035.7060932
ACGGGTC1550.034.9518816
TACTGTT1151.2292157E-534.93902670
ATCAACG23450.034.772263
TCAACGC23700.034.2627034
CAACGCA23850.034.051445
CGACCAT400.00285342533.840726
AACGCAG24000.033.840726
ACGCAGA25000.032.351737
CGCAGAG25450.031.7796948
AGAGTAC25350.031.77156311
GCAGAGT25750.031.5408659
Produced by FastQC (version 0.11.5)