FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1326503
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAACACTATGGTAGCTCGCTCCCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGG60520.45623718906025845No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50250.3788155774996363No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49520.3733123860255122No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA37110.27975813096540303No Hit
GTGTATTACTGTCAACACTATGGTAGCTCGCTCCCTTTCGGCGGAGGGAC33860.25525762097786436No Hit
CAGTAATACACTGCAAAATCCTCAGCCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA32170.24251735578434425No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA29480.22223847213311992No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28200.21258904050725858No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28190.21251365432268152No Hit
GTAATACACTGCAAAATCCTCAGCCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA28140.21213672339979633No Hit
ACTGTGGACAGTGGGGTGGAAAGGCAACCTCCGATTTTTGGAGTGGCCCCTTTGACTTCTGGGGCCAGGGAACC27710.20889511746298348No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG27240.20535196678786255No Hit
CCTCATGTATGGTGCATCTATTAGGGCCACTGGCATCCCAGAGAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT26860.20248729177393493No Hit
GTGTACTACTGTGGACAGTGGGGTGGAAAGGCAACCTCCGATTTTTGGAG26700.20128111282070224No Hit
ATGGTAGCTCGCTCCCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA26460.19947184439085322No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26310.19834105162219762No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25570.1927624739634965No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25270.19050088842618523No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25180.18982241276499187No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGCCAA24950.1880885305197199No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24900.18771159959683467No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGCCAACAGCTTGGCCTGGTACCGCCAGAGACCTGGCCAGGCTCCCAGGCT24680.18605310353613974No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGGCTGAGGATT24080.18152993246151725No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23870.17994682258539935No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG23810.1794945054779371No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC23280.17549903769535388No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22800.17188050083565587No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGCCAACAGCTTGGCCTGGTA22100.16660346791526293No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21420.16147720736402407No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21020.15846175998094236No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC20610.15537092641328365No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20480.15439090601378208No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG20340.1533354994297035No Hit
AGCTACCATAGTGTTGACAGTAATACACTGCAAAATCCTCAGCCTCCAGTCTGCTGATGGTGAGAGTGAAGT20220.152430865214779No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20100.15152623099985452No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT19910.1500938934928907No Hit
ACCATAGTGTTGACAGTAATACACTGCAAAATCCTCAGCCTCCAGTCTGC19800.14926464546254323No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19320.14564610860284521No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19170.1445153158341896No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC19030.143459909250111No Hit
GTCCCAGACCCACTGCCACTGAACCTCTCTGGGATGCCAGTGGCCCTAAT18990.14315836451180283No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18880.14232911648145538No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18570.13999214475956706No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18310.13803210396056398No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT18290.1378813315914099No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC17890.1348658842083282No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17750.1338104776242496No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17500.13192582300982358No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT17440.13147350590236134No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG17110.12898576181131893No Hit
CTATGGTAGCTCGCTCCCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT17090.12883498944216484No Hit
GTACTACTGTGGACAGTGGGGTGGAAAGGCAACCTCCGATTTTTGGAGTG17050.12853344470385666No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTCTCTGGGATGCCA17010.1282318999655485No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGGG16680.12574415587450613No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16580.12499029402873571No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGGCTGAGGATTTTGCAGTGTATTACTGTCAACACTATGGTAGCTCG16490.12431181836754232No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC16240.12242716375311627No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16160.12182407427649994No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16130.12159791572276882No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16070.12114559861530656No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16050.12099482624615247No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15960.12031635058495911No Hit
CCCCTGGACAAGGACCTGAGTGGATGGGAGGGATCATCCCTACCTTTGGT15840.1194117163700346No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15760.11880862689341826No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15730.11858246833968712No Hit
GTCAGAGTGTTGCCAACAGCTTGGCCTGGTACCGCCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATGTATGGTG15640.11790399267849376No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC15550.11722551701730038No Hit
CATGTATGGTGCATCTATTAGGGCCACTGGCATCCCAGAGAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA15480.11669781372526108No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC15170.11436084200337278No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15170.11436084200337278No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15020.11323004923471715No Hit
CTATTAGGGCCACTGGCATCCCAGAGAGGTTCAGTGGCAGTGGGTCTGGG14800.11157155317402222No Hit
GTCTACATGGAGCTGACCAACCTGAGATCTGAAGACGCGGCCGTGTACTA14590.10998844329790435No Hit
GTCCTGGAACTTCTGTGCGTAGTTTGGTGTACCAAAGGTAGGGATGATCC14440.1088576505292487No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14180.10689760973024562No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC14180.10689760973024562No Hit
GTCCCAGGTGCAGCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGG13980.10538988603870478No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13660.10297752813223943No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13620.10267598339393127No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13580.10237443865562308No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)