FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1326503
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAACACTATGGTAGCTCGCTCCCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGG58540.44131072451400416No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50210.3785140327613281No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42040.3169235199618847No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36580.2757626631828198No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA34500.26008233679079507No Hit
GTGTATTACTGTCAACACTATGGTAGCTCGCTCCCTTTCGGCGGAGGGAC32960.24847286436593058No Hit
CAGTAATACACTGCAAAATCCTCAGCCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA30850.2325663794201747No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27930.21055361352367843No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27270.20557812534159367No Hit
GTAATACACTGCAAAATCCTCAGCCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA26830.2022611332202038No Hit
ACTGTGGACAGTGGGGTGGAAAGGCAACCTCCGATTTTTGGAGTGGCCCCTTTGACTTCTGGGGCCAGGGAACC26150.19713487266896493No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25770.19427019765503734No Hit
GTCCACAGTAGTACACGGCCGCGTCTTCAGATCTCAGGTTGGTCAGCTCCATGTAGACTGTGGTCGTGGGCTCGT25460.19193322593314904No Hit
GTGTACTACTGTGGACAGTGGGGTGGAAAGGCAACCTCCGATTTTTGGAG25330.1909532055336475No Hit
CCTCATGTATGGTGCATCTATTAGGGCCACTGGCATCCCAGAGAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT25320.19087781934907044No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25030.1886916199963362No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGCCAACAGCTTGGCCTGGTACCGCCAGAGACCTGGCCAGGCTCCCAGGCT24590.18537462787494638No Hit
ATGGTAGCTCGCTCCCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA24250.18281149759932697No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGGCTGAGGATT23700.17866525744758965No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGCCAA23410.17647905809485542No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23230.17512210677246867No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23220.1750467205878916No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC23200.17489594821873752No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22460.16931737056003643No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22300.16811119160680374No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGCCAACAGCTTGGCCTGGTA22050.1662265369923777No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC21740.1638895652704894No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21280.16042180077994544No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21220.1599694836724832No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21180.15966793893417505No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20860.1572555810277097No Hit
GTAGTACACGGCCGCGTCTTCAGATCTCAGGTTGGTCAGCTCCATGTAGA20670.1558232435207459No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20640.15559708496701477No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20570.15506938167497547No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20390.15371243035258872No Hit
GTCCCAGACCCACTGCCACTGAACCTCTCTGGGATGCCAGTGGCCCTAAT19920.15016927967746774No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19690.14843539743219578No Hit
ACCATAGTGTTGACAGTAATACACTGCAAAATCCTCAGCCTCCAGTCTGC19430.1464753566331927No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19200.14474147438792073No Hit
AGCTACCATAGTGTTGACAGTAATACACTGCAAAATCCTCAGCCTCCAGTCTGCTGATGGTGAGAGTGAAGT18850.14210295792772426No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18830.14195218555857017No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18750.1413490960819538No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18610.14029368949787524No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17770.1339612499934037No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC17570.13245352630186288No Hit
GTCTACATGGAGCTGACCAACCTGAGATCTGAAGACGCGGCCGTGTACTA17480.1317750506406695No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC17390.1310965749794761No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17290.13034271313370568No Hit
CAGTAGTACACGGCCGCGTCTTCAGATCTCAGGTTGGTCAGCTCCATGTA17050.12853344470385666No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17020.12830728615012557No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT16820.1267995624585847No Hit
CCTTGGGGCTGGTCGGGGATGCTGCGGAGACGGAGACCAGGGTTCCCTGG16560.12483952165958162No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC16530.12461336310585049No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG16350.12325641178346375No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTCTCTGGGATGCCA16320.12303025322973261No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGGCTGAGGATTTTGCAGTGTATTACTGTCAACACTATGGTAGCTCG16170.12189946046107698No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16130.12159791572276882No Hit
CTATGGTAGCTCGCTCCCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT16080.1212209847998836No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT15700.118356309785956No Hit
GTCAGAGTGTTGCCAACAGCTTGGCCTGGTACCGCCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATGTATGGTG15690.11828092360137896No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC15610.11767783412476264No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15560.11730090320187742No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15480.11669781372526108No Hit
GTACTACTGTGGACAGTGGGGTGGAAAGGCAACCTCCGATTTTTGGAGTG15460.116547041356107No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15260.11503931766456617No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGGG15220.11473777292625797No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC14960.11277773212725489No Hit
GTCCTGGAACTTCTGTGCGTAGTTTGGTGTACCAAAGGTAGGGATGATCC14960.11277773212725489No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG14680.11066691895909772No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14670.11059153277452066No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC14380.10840533342178645No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14370.10832994723720941No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG14160.10674683736109153No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14010.10561604459243591No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13950.10516372748497366No Hit
CATGTATGGTGCATCTATTAGGGCCACTGGCATCCCAGAGAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA13930.10501295511581955No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13750.10365600379343282No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13680.1031283005013935No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13640.10282675576308534No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13570.10229905247104605No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13410.10109287351781338No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13290.10018823930288887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10200.044.356651
GTATCAA24600.038.0281221
GATTCAC2000.032.3169861
AATGGTA3850.031.99118670
TATCAAC29500.031.686482
TACGTAC652.5694038E-531.37744712
ATCAACG30800.030.7858643
GTGCTAG1001.6632839E-830.6160931
TCAACGC30900.030.4662574
TTCGCTA1351.0913936E-1130.2153198
TTGTTCG1351.0913936E-1130.2141785
CAACGCA31200.030.1778645
TAACCGT703.3652388E-530.16187753
AACGCAG31350.030.1430366
GACAGGT1950.029.6566141
AGAGTAC32450.029.43549311
CGCAGAG32400.029.3760058
ACGCAGA32400.029.2710917
GCTATCG1401.8189894E-1129.13620211
ATACGTA704.261401E-529.13620211
Produced by FastQC (version 0.11.5)