FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822522
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT80790.9822229678962994No Hit
CTATAATATTGCTGACAGTAGTAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC72230.8781528032076954No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47940.5828415531742616No Hit
CTTTTATACAGCTCCAACAATAAGAAGTACTTAGCTTGGTTGCAGCAGAA45830.5571887438877015No Hit
CCTTAGGAGGCTGTCCTGGTTTCTGCTGCAACCAAGCTAAGTACTTCTTATTGTTGGAGCTGTATAAAAGACT44330.5389521496081563No Hit
CTCCTAAGGTGCTCATTTACTGGGCATCCACCCGGGAATCCGGGGTCCCT35610.4329367481964008No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35190.4278305017981282No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31030.37725434699618976No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29750.3616924532109779No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28350.3446716318834025No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26540.3226661414527514No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24480.29762121864217617No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23570.2865576847792521No Hit
GTATAAAAGACTCTGGTTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA23250.28266721133294914No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22920.27865516059144924No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21900.2662542764813586No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21760.26455219434860106No Hit
GTAGTAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA20970.2549475880280406No Hit
GTTTACTACTGTCAGCAATATTATAGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA19850.24133093096598024No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19240.23391471595896524No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC19080.23196947923581376No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19020.23124001546463196No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19010.23111843816943498No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18870.22941635603667743No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18740.22783585119911684No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18470.22455326422879873No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18240.2217569864392685No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17460.21227395741390503No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAACCAGAGTCTTTTATA16820.20449301052129915No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC16080.19549629067672353No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16050.19513155879113264No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC15920.19355105395357206No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15350.18662114812734493No Hit
TCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC15290.1858916843561631No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT14420.17531445967402695No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG14040.17069452245654218No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13960.16972190409496646No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAACCAGAGTCTTTTATACAGCTCCAA13600.16534512146787564No Hit
AACCAGGACAGCCTCCTAAGGTGCTCATTTACTGGGCATCCACCCGGGAA13580.1651019668774817No Hit
GTACTTAGCTTGGTTGCAGCAGAAACCAGGACAGCCTCCTAAGGTGCTCATTTACTGGGCATCCACCCGGGAATC13420.16315673015433022No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13350.16230568908795143No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13120.1595094112984212No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATGTGGCAGTTTACTACTGTCAGCAATATTATAGTGCT13040.15853679293684547No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12850.1562268243281031No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12790.15549736055692126No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12730.15476789678573946No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12670.15403843301455766No Hit
ATAGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA12380.1505126914538456No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11790.14333963103722455No Hit
GGCTGAGGATGTGGCAGTTTACTACTGTCAGCAATATTATAGTGCTCCGT11760.14297489915163364No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAAC11490.1396923121813155No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11370.1382333846389519No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11290.13726076627737618No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11290.13726076627737618No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11220.1364097252109974No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11070.13458606578304289No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11050.13434291119264896No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATGTGGCAGTTTA10990.13361344742146716No Hit
CAGTAGTAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA10840.13178978799351265No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT10710.13020928315595207No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10710.13020928315595207No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10620.12911508749917935No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10500.12765615995681576No Hit
GTCAGGGACCCCGGATTCCCGGGTGGATGCCCAGTAAATGAGCACCTTAGGAGGCTGTCCTGGTTTCTGCTGCAA10480.1274130053664218No Hit
GGATCTCTGGTGCCTACGGGGACGTCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG10470.12729142807122484No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10430.12680511889043697No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10400.12644038700484606No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10350.12583250052886122No Hit
GAGCACTATAATATTGCTGACAGTAGTAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT10310.12534619134807337No Hit
CATTTACTGGGCATCCACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCA10140.12327937732972492No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAACCAGAGTCTTTTATACAGCTCCAACAATAAGAAGTA9960.12109098601617951No Hit
GTCCTGGTTTCTGCTGCAACCAAGCTAAGTACTTCTTATTGTTGGAGCTG9700.11792997634105837No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9590.1165926260938917No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9490.11537685314192203No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC9490.11537685314192203No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9390.11416108018995237No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9310.11318846182837664No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG9210.11197268887640695No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATG9160.11136480240042212No Hit
CACCATCAGCAGCCTGCAGGCTGAGGATGTGGCAGTTTACTACTGTCAGCAATATTATAGTGCTCCGTGGACGTT9100.1106353386292403No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9060.11014902944845244No Hit
TTACTACTGTCAGCAATATTATAGTGCTCCGTGGACGTTCGGCCAAGGGA9050.11002745215325548No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC8980.10917641108687672No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8960.10893325649648276No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8900.10820379272530098No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8710.1058938241165586No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8690.10565066952616464No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8660.10528593764057374No Hit
GGATGTGGCAGTTTACTACTGTCAGCAATATTATAGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAAT8630.10492120575498284No Hit
TTATAGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT8620.10479962845978588No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8590.10443489657419498No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACGTCGTGATGA8570.10419174198380103No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT8450.10273281444143745No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAT150.002437544368.4052935
GTCGTAG250.006516685753.36268669
ATCCGTC200.007112686552.19609546
CCTAGTC200.00793778850.76711316
ACTAGAT200.00796263550.7269943
GTGGTAT2000.049.101761
ATTGACG306.673593E-445.4804932
TGGTATC2250.045.09342
GGTATCA7650.042.9377561
GAATTGA350.001440053138.89979630
GTTATTA750.001929149536.647370
ACAGGTA853.5597623E-935.8552441
GTATCAA18350.034.8782581
GTGCTCA19650.033.9197370
TTTCGAT850.003563731732.33585470
TATCAAC20450.030.5951562
AACGCAG21300.029.850566
CGGCTTA500.005276596629.84674366
TCAACGC21100.029.8111214
ATCAACG21100.029.6508463
Produced by FastQC (version 0.11.5)