FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047850
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT64210.6127785465476929No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39580.3777258195352388No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36690.3501455360977239No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26820.2559526649806747No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25260.2410650379348189No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24870.23734313117335495No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24560.23438469246552465No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA24060.22961301713031443No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23930.2283723815431598No Hit
CATCTATGGTGCGTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAATGGCATTGGGTCTGGGGCAGAGTTCA23490.2241733072481748No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22880.21835186333921838No Hit
GTATGCTAACTGGCCTCCGTGGACGTTCGGCCACGGGACCAAGGTGGAAATCACACGAACTGTGGCTGCACCAT19110.18237343131173356No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18850.17989216013742426No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18740.178842391563678No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18660.17807892351004437No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18520.17674285441618554No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC17520.16719950374576514No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17480.16681776971894832No Hit
GTTTATTACTGTCAGCAGTATGCTAACTGGCCTCCGTGGACGTTCGGCCA17040.16261869542396334No Hit
CTGGTATCCCAGCCAGGTTCAATGGCATTGGGTCTGGGGCAGAGTTCACT15960.15231187669990934No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15590.1487808369518538No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15210.14515436369709403No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATCTTGCAGTTTATTACTGTCAGCAGTATGCTAACTGG15130.14439089564346042No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCACCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT15000.14315026005630577No Hit
TCTATGGTGCGTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAATGGCATTGGGTCTGGGGCAGAGTTCACT14840.1416233239490385No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14800.1412415899222217No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCACCAACTTAGCCTGGTA14660.13990552082836283No Hit
GTTAGCATACTGCTGACAGTAATAAACTGCAAGATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGC14640.13971465381495443No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14590.13923748628143343No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAC14320.13666078160041992No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14170.13522927899985684No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13950.13312974185236437No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13780.1315073722383929No Hit
GTAATAAACTGCAAGATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGA13720.13093477119816768No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13080.12482702676909863No Hit
GTGCAGCCACAGTTCGTGTGATTTCCACCTTGGTCCCGTGGCCGAACGTCCACGGAGGCCAGTTAGCATACTG13030.12434985923557762No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12710.12129598702104308No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12620.12043708546070526No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC12440.11871928234002957No Hit
GCCACAGTTCGTGTGATTTCCACCTTGGTCCCGTGGCCGAACGTCCACGG12410.11843298181991697No Hit
GTCAGAGTGTTGGCACCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTG12160.11604714415231188No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTGATTTCCACCTTGGTCC12160.11604714415231188No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12100.11547454311208666No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11920.11375673999141098No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11700.1116572028439185No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG11640.11108460180369328No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11350.10831703010927138No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11280.10764899556234193No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11130.10621749296177888No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11060.10554945841484945No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11010.10507229088132844No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10980.10478599036121582No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT10910.10411795581428639No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10740.10249558620031493No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10620.10135038411986448No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGATTCAGGGAGA10580.10096865009304766No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10530.10049148255952664No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10520.10039604905282246No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)