FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047850
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG101650.970081595648232No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36350.346900796869781No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34760.33172686930381257No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29170.27837953905616264No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25040.23896550078732642No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23720.22636827790237152No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22910.218638163859331No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22640.21606145917831748No Hit
CATCTATGGTGCGTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAATGGCATTGGGTCTGGGGCAGAGTTCA22290.2127212864436704No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19620.18724054015364794No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19360.18475926897933864No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19280.18399580092570503No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18670.17817435701674858No Hit
GTATGCTAACTGGCCTCCGTGGACGTTCGGCCACGGGACCAAGGTGGAAATCACACGAACTGTGGCTGCACCAT17830.17015794245359545No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17550.16748580426587775No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17360.1656725676384979No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16950.16175979386362552No Hit
GTTTATTACTGTCAGCAGTATGCTAACTGGCCTCCGTGGACGTTCGGCCA16160.15422054683399342No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC15610.14897170396526221No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15270.14572696473731928No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14750.14076442238870068No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCACCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT14740.14066898888199647No Hit
CTGGTATCCCAGCCAGGTTCAATGGCATTGGGTCTGGGGCAGAGTTCACT14690.14019182134847544No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14440.13780598368087035No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13880.13246170730543494No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13800.1316982392518013No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATCTTGCAGTTTATTACTGTCAGCAGTATGCTAACTGG13750.1312210717182803No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCACCAACTTAGCCTGGTA13720.13093477119816768No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAC13700.13074390418475929No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13610.12988500262442143No Hit
TCTATGGTGCGTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAATGGCATTGGGTCTGGGGCAGAGTTCACT13590.12969413561101303No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13400.12788089898363317No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC13240.12635396287636588No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13070.12473159326239444No Hit
GTTAGCATACTGCTGACAGTAATAAACTGCAAGATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGC13010.1241589922221692No Hit
GTGCAGCCACAGTTCGTGTGATTTCCACCTTGGTCCCGTGGCCGAACGTCCACGGAGGCCAGTTAGCATACTG12950.12358639118194399No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12690.12110512000763468No Hit
GTAATAAACTGCAAGATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGA12450.11881471584673378No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12390.11824211480650856No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12240.11681061220594552No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11630.11098916829698907No Hit
GTCAGAGTGTTGGCACCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTG11230.10717182802882093No Hit
GCCACAGTTCGTGTGATTTCCACCTTGGTCCCGTGGCCGAACGTCCACGG11200.10688552750870832No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTGATTTCCACCTTGGTCC10910.10411795581428639No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT10800.10306818724054015No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10790.10297275373383596No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10650.10163668463997709No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10640.1015412511332729No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10610.10125495061316027No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10510.10030061554611824No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTAT151.5847573E-4136.052370
ATTACGC150.002343107769.0916240
GGTATCA13250.044.3737831
GTATCAA33800.038.0703051
CTCTTAT400.002956604433.5972941
TAGTACA601.6175376E-533.5732464
ATCAACG39700.032.220173
TATCAAC40500.031.6726632
TCAACGC40200.031.6523864
GATTTAG1302.5556701E-531.39668570
CAACGCA40700.031.3490185
AACGCAG41150.031.0106376
CGCAGAG42800.029.8935958
ACGCAGA42900.029.7456387
AGAGTAC43150.029.73036411
TACCGCA855.462067E-627.6551537
CAGAGTA46900.027.20999110
GTCGCTA650.00833391527.18023969
AATGGTA2057.035942E-726.5467970
TTGTATC650.001007456825.8576517
Produced by FastQC (version 0.11.5)