FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1487352
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAATATGTTACTTCACCTCACACTTTTGGCCAGGGGA63920.42975704473453497No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45220.3040302497324104No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42630.28661675245671503No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGCTGAGAGAGAAGTCTGTCCCAGACCCACTGCCA39370.264698605306612No Hit
CATCTATAGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCT36420.24486469914317527No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31510.21185301125759068No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA31090.20902920088855897No Hit
GTGTATTACTGTCAGCAATATGTTACTTCACCTCACACTTTTGGCCAGGGGACCAAGCTGGAAATCAAACGAA30580.20560028829759197No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28660.1926914408963043No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28590.19222080583479903No Hit
CTATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGATACCAGGCTAAGTAGCTGATGGGAACACTTTGA28360.1906744334898531No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28300.19027103200856288No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG27210.18294257176512352No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26250.1764881480644797No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25560.17184903102964194No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25030.16828565127824482No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24270.1631758991819018No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23920.1608227238743754No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGATC23740.15961251943050467No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGCTGAGAGAGAAGTCTGTCCCAGACCCACTGCCA23360.15705764338233316No Hit
CTCCTGCAGGGCCAGTCAAAGTGTTCCCATCAGCTACTTAGCCTGGTATC23260.15638530758018276No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAAAGTGTTCCCAT22760.15302362856943078No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22710.15268746066835556No Hit
GTCTGGGACAGACTTCTCTCTCAGCATCAGCAGACTGGAGCCTGAAGATT22690.1525529935079255No Hit
ATAATAGACGGCCGTGTCCGCGGCAGTCACGGACCTCAGTCTCAGGGAGAACTGGTTCTCAGACTTGTCTACGGA22650.15228405918706533No Hit
CAATAATAGACGGCCGTGTCCGCGGCAGTCACGGACCTCAGTCTCAGGGA22290.14986365029932391No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG22260.1496619495586788No Hit
CTTTTGGCCAGGGGACCAAGCTGGAAATCAAACGAACTGTGGCTGCACCA21910.14730877425115238No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21830.14677090560943207No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21730.14609856980728167No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21550.14488836536341096No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21140.1421317885745943No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20540.1380977737616919No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20390.13708927005846633No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20140.13540843055309032No Hit
GTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAAA20130.1353411969728753No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19970.13426545968943465No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19800.13312248882577896No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19720.13258462018405864No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19590.13171058364126312No Hit
GTGCAGCCACAGTTCGTTTGATTTCCAGCTTGGTCCCCTGGCCAAAAGTG19420.13056761277760745No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT19420.13056761277760745No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19240.12935740833373674No Hit
CCGTAGTAGTAGTCCCTCGAAGCCCTCGCACAATAATAGACGGCCGTGTC18900.12707146660642538No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGC18650.12539062710104937No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCAGCTTGGTCC18520.12451659055825386No Hit
GGCCAAAAGTGTGAGGTGAAGTAACATATTGCTGACAGTAATACACTGCA18520.12451659055825386No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT18410.12377702117588843No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18360.12344085327481323No Hit
CTATTATTGTGCGAGGGCTTCGAGGGACTACTACTACGGTTTGGACGTCTGGGGCCAGGGGACCACAGTCATCGT17810.11974300636298603No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17700.1190034369806206No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG17190.11557452438965357No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17140.11523835648857836No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17030.11449878710621292No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16960.11402815204470765No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC16930.1138264513040625No Hit
GTCAAAGTGTTCCCATCAGCTACTTAGCCTGGTATCAGCAGAAACCTGGC16850.1132885826623422No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16740.11254901327997675No Hit
GTCTCTGGTGGCTCCATCAGTCGTGCTAGTTACTACTGGAGCTGGATCCG16470.11073370661417069No Hit
CTACTACGGTTTGGACGTCTGGGGCCAGGGGACCACAGTCATCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGT15980.10743926118363374No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15960.10730479402320364No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15690.10548948735739758No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT15570.1046826843948171No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT15570.1046826843948171No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15480.10407758217288174No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15410.10360694711137647No Hit
GTCTATTATTGTGCGAGGGCTTCGAGGGACTACTACTACGGTTTGGACGT15210.10226227550707567No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15030.10105207106320495No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC14900.10017803452040944No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGATGACTGTGGTCCCCTGG14890.10011080094019438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGTG28150.042.759370
CGTATGC2500.041.4779741
TCGTATG2500.040.03256240
TACGCTG350.001345862339.44094540
ATGCCGT2500.038.86921344
GTATCAA28350.036.317611
ACGAGAC2850.035.8763522
CATGAAT2950.034.81711632
CTTATAC2900.034.0928541
TATACAC3000.034.0664943
CCCACGA3050.033.51478619
AATCTCG2900.033.1660636
ATCTCGT2800.033.15635337
GAGACGC3100.032.99308424
CGAGACG3100.032.98753423
AGACGCT3150.032.47375525
GACGCTC3200.031.9749626
TAGTAGC450.00376993431.97198363
TGTTAGA12300.031.9107770
GAATCTC3050.031.50397535
Produced by FastQC (version 0.11.5)