FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1487352
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAATATGTTACTTCACCTCACACTTTTGGCCAGGGGA60990.4100576057315283No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44330.2980464610932718No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38090.25609270703908693No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGCTGAGAGAGAAGTCTGTCCCAGACCCACTGCCA37550.25246209370747474No Hit
CATCTATAGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCT35680.2398894142072623No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34350.23094734803866201No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA29680.1995492660782384No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29370.19746502509157215No Hit
GTGTATTACTGTCAGCAATATGTTACTTCACCTCACACTTTTGGCCAGGGGACCAAGCTGGAAATCAAACGAA29250.19665822212899167No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28890.19423781324125022No Hit
CTATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGATACCAGGCTAAGTAGCTGATGGGAACACTTTGA26710.17958089275437153No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26060.17521071004039393No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25690.17272306757243747No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGATC25430.17097499448684642No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23630.15887295004813926No Hit
GTCTGGGACAGACTTCTCTCTCAGCATCAGCAGACTGGAGCCTGAAGATT23350.15699040980211812No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23040.15490616881545188No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22980.15450276733416166No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22570.15174619054534502No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22520.1514100226442698No Hit
ATAATAGACGGCCGTGTCCGCGGCAGTCACGGACCTCAGTCTCAGGGAGAACTGGTTCTCAGACTTGTCTACGGA22430.15080492042233445No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGCTGAGAGAGAAGTCTGTCCCAGACCCACTGCCA22420.15073768684211944No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22380.15046875252125927No Hit
CAATAATAGACGGCCGTGTCCGCGGCAGTCACGGACCTCAGTCTCAGGGA21640.1454934675853463No Hit
CTCCTGCAGGGCCAGTCAAAGTGTTCCCATCAGCTACTTAGCCTGGTATC21560.14495559894362597No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21360.14361092733932518No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21340.14347646017889512No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20720.13930797820556265No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20600.13850117524298217No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAAAGTGTTCCCAT20510.1378960730210468No Hit
CTTTTGGCCAGGGGACCAAGCTGGAAATCAAACGAACTGTGGCTGCACCA20490.13776160586061673No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20450.13749267153975656No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19860.1335258903070692No Hit
GTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAAA19770.13292078808513386No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19320.12989527697545705No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT19260.12949187549416682No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19200.12908847401287657No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19200.12908847401287657No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGC18580.1249199920395441No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18500.12438212339782379No Hit
CTATTATTGTGCGAGGGCTTCGAGGGACTACTACTACGGTTTGGACGTCTGGGGCCAGGGGACCACAGTCATCGT18490.12431488981760874No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18470.12418042265717867No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18280.12290298463309292No Hit
GTGCAGCCACAGTTCGTTTGATTTCCAGCTTGGTCCCCTGGCCAAAAGTG17910.12041534216513644No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17580.11819663401804012No Hit
GGCCAAAAGTGTGAGGTGAAGTAACATATTGCTGACAGTAATACACTGCA17440.11725536389502955No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17210.11570899155008364No Hit
GTCTCTGGTGGCTCCATCAGTCGTGCTAGTTACTACTGGAGCTGGATCCG17190.11557452438965357No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17150.1153055900687934No Hit
CCGTAGTAGTAGTCCCTCGAAGCCCTCGCACAATAATAGACGGCCGTGTC17050.11463325426664299No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17010.11436431994578285No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCAGCTTGGTCC16950.1139609184644926No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC16510.11100264093503084No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT15960.10730479402320364No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15910.10696862612212844No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15790.10616182315954799No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG15580.10474991797503214No Hit
CTACTACGGTTTGGACGTCTGGGGCCAGGGGACCACAGTCATCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGT15570.1046826843948171No Hit
GTCAAAGTGTTCCCATCAGCTACTTAGCCTGGTATCAGCAGAAACCTGGC15410.10360694711137647No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGC150.001986874372.02659663
AGGTACG451.0896257E-437.789492
GTATCAA29150.037.2579151
AACTGTG28750.034.3191270
TATCAAC35200.030.628812
TTTTCGC450.004957768630.231592
ATCAACG36100.029.9553953
TCAACGC37150.029.1992954
AACGCAG37350.029.0478256
CAACGCA37550.028.7986645
CGCAGAG39300.027.6930628
ACGCAGA39200.027.6769477
GACCGTA500.00825206127.236821
GCAGAGT40300.027.090289
AGAGTAC40250.027.03943611
GAGTTTA6150.026.572511
AGTTTAT6050.025.8592532
GATATAG801.0539023E-425.5079062
GAACTGT24900.024.76449469
GCTTGTG1251.4379111E-724.513141
Produced by FastQC (version 0.11.5)