FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453859
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17340.3820569824549034No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17120.3772096620316001No Hit
GTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC16750.36905735041059007No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA13490.29722887504709616No Hit
GGTACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT11750.2588909771536975No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG11450.2522809947582839No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC11190.24655234334892556No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10660.23487470778369496No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10060.22165474299286783No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCA9820.21636675707653702No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9470.20865511094855452No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG9440.20799411270901313No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9280.2044687887647926No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9250.20380779052525125No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGA9050.19940113559497552No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9010.19851980460892038No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA8690.19146915672047926No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7970.17560519897148674No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7970.17560519897148674No Hit
AGGTACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT7960.17538486622497296No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7940.1749442007319454No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7910.17428320249240403No Hit
GTACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGA7640.16833421833653184No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCCTCTGAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA7390.1628258996736872No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7250.15974124122249422No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7240.1595209084759804No Hit
GCTAATGCTCTGGGTCCCAGGATCCACTGGGGATGTTGTGATGACTCAGT7100.15643625002478742No Hit
CAATAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGA7070.15577525178524607No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCA7050.15533458629221852No Hit
CTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATT7020.15467358805267714No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTG7020.15467358805267714No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCACTGGGGATGTTGTGATGA6990.1540125898131358No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT6930.15269059333405308No Hit
GTCTCTGGTGCCTCCATCAGTAGTTACTACTGGAACTGGATCCGGCAGCC6900.15202959509451175No Hit
ATAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGAACTGGCTCTTGGACGTGTCTACAGA6900.15202959509451175No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC6870.1513685968549704No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6860.15114826410845658No Hit
CGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAG6740.14850427115029116No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT6700.14762294016423602No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTCAGAGGGTTTCTAACCGGGAC6580.1449789472060706No Hit
GTCCCAGACAGATTCACCGGCAGTGGGTCAGGCACTGATTTCACACTGAA6500.14321628523396032No Hit
GGCCAAAAGTGTACGGCCAGTGTGTACCTTGCATGCAGTAATAAACCCCA6470.14255528699441897No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG6430.14167395600836383No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC6310.1390299630501984No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTATA6100.13440297537340892No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCC6100.13440297537340892No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA5950.13109798417570215No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCACTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC5940.13087765142918836No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5910.130216653189647No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTCAGAGGGTTT5700.12558966551285752No Hit
GGTCTAGTCAAAGCCTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTC5610.12360667079423346No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT5560.12250500706166452No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5550.12228467431515075No Hit
GTATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGGAGA5540.12206434156863696No Hit
GTATTATTGTGCGAGAGGCGAAAATCATAACTGGAACTATGACTCCTGGGGCCCGGGAACCCTGGTCACTGTCTC5490.12096267783606802No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC5420.11942034861047153No Hit
GTTTCTAACCGGGACTCTGGGGTCCCAGACAGATTCACCGGCAGTGGGTC5420.11942034861047153No Hit
CTACAGTGGGGGCACCAGCTACAACCCCTCCCTCAACAGTCGAGTCACCA5380.11853901762441639No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC5350.11787801938487504No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG5240.1154543591732234No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5240.1154543591732234No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5230.11523402642670963No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5170.1139120299476269No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5010.11038670600340633No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT4990.10994604051037878No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA4980.109725707763865No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC4970.10950537501735119No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCACTGGGGATGTTGTGATGACTCAGTCTCCACT4900.1079630457917547No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA4870.10730204755221334No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC4870.10730204755221334No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC4830.1064207165661582No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA4800.10575971832661685No Hit
CACCTGCACTGTCTCTGGTGCCTCCATCAGTAGTTACTACTGGAACTGGATCCGGCAGCCCCCAGGGAAGGGA4770.1050987200870755No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT4700.10355639086147901No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC4640.1022343943823963No Hit
GTTAGAAACCCTCTGAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCT4580.10091239790331358No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG4580.10091239790331358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3100.051.88471
GTAATAT200.007625282351.281843
CGTATGC1000.048.34774841
GTGGTAT507.388917E-847.9105531
GACTAGC1000.047.88942726
AGACTAG1000.047.8788725
CTAGCGC1050.045.62909728
ACTAGCG1050.045.6089827
CGAGACT1050.045.59390323
TCGTATG1000.044.83944740
TAGCGCT1100.043.56465529
GAGACTA1100.043.52145424
GCCGTCT1050.042.99575446
GTATGCC1050.042.7993842
ATCTCGT1050.042.57646637
ACGAGAC1100.040.40832522
CACGAGA1100.040.40387321
CGCTCAT1200.039.99603332
AGCGCTC1200.039.9430830
CTTATAC1200.039.925461
Produced by FastQC (version 0.11.5)