FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453859
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17800.3921922887945375No Hit
GTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC16920.3728030071013244No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14640.32256714089618144No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13800.3040591901890235No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG11510.2536029912373667No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA11150.2456710123628704No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC10410.22936638912085028No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10200.22473940144406082No Hit
GGTACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT10120.22297673947195054No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCA9440.20799411270901313No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8950.1971978081298377No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8830.19455381517167225No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8490.18706250179020356No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA8350.18397784333901057No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG8330.183537177845983No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGA8230.18133385038084515No Hit
GTACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGA7990.1760458644645143No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7900.17406286974589025No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7450.1641478961527699No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7440.16392756340625614No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCCTCTGAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA7360.16216490143414586No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7320.16128357044809072No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTG7170.15797857925038392No Hit
AGGTACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT7150.15753791375735637No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7100.15643625002478742No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7070.15577525178524607No Hit
CTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATT6890.15180926234799794No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC6840.15070759861542904No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6750.14872460389680495No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6720.1480636056572636No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCA6680.14718227467120845No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6470.14255528699441897No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6410.14123329051533626No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC6380.1405722922757949No Hit
GCTAATGCTCTGGGTCCCAGGATCCACTGGGGATGTTGTGATGACTCAGT6370.14035195952928112No Hit
GGCCAAAAGTGTACGGCCAGTGTGTACCTTGCATGCAGTAATAAACCCCA6280.13836896481065705No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG6180.1361656373455192No Hit
CAATAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGA6140.13528430635946406No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCACTGGGGATGTTGTGATGA6030.13286064614781243No Hit
ATAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGAACTGGCTCTTGGACGTGTCTACAGA5780.12735232748496778No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTATA5770.12713199473845402No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA5720.1260303310058851No Hit
GTCCCAGACAGATTCACCGGCAGTGGGTCAGGCACTGATTTCACACTGAA5670.12492866727331615No Hit
GTCTCTGGTGCCTCCATCAGTAGTTACTACTGGAACTGGATCCGGCAGCC5640.1242676690337748No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC5600.12338633804771966No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCC5600.12338633804771966No Hit
CGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAG5600.12338633804771966No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCACTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC5530.12184400882212316No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC5530.12184400882212316No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT5510.12140334332909561No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT5440.11986101410349911No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA5400.11897968311744397No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG5400.11897968311744397No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTCAGAGGGTTTCTAACCGGGAC5310.1169966883988199No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5280.11633569015927854No Hit
GTTTCTAACCGGGACTCTGGGGTCCCAGACAGATTCACCGGCAGTGGGTC5250.11567469191973719No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5230.11523402642670963No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT5210.11479336093368205No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT5110.1125900334685442No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5090.11214936797551663No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC5030.11082737149643392No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC4980.109725707763865No Hit
GTATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGGAGA4950.10906470952432364No Hit
GTTTATTACTGCATGCAAGGTACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAA4910.10818337853826851No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA4860.10708171480569957No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG4860.10708171480569957No Hit
CTACAGTGGGGGCACCAGCTACAACCCCTCCCTCAACAGTCGAGTCACCA4840.106641049312672No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4820.10620038381964443No Hit
GGTCTAGTCAAAGCCTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTC4780.10531905283358929No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4740.10443772184753415No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTCAGAGGGTTT4740.10443772184753415No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT4720.10399705635450657No Hit
GTATTATTGTGCGAGAGGCGAAAATCATAACTGGAACTATGACTCCTGGGGCCCGGGAACCCTGGTCACTGTCTC4700.10355639086147901No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCACTGGGGATGTTGTGATGACTCAGTCTCCACT4660.10267505987542386No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT4630.10201406163588252No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCA100.009672008130.6759570
TAGGTAT200.00752320551.4569132
GTGGTAT601.07320375E-1051.229931
GGTATCA3650.049.5924451
AATGGTA1102.2455606E-741.57871270
GACAGGT1000.040.9839441
ATCAGTG8300.039.36022670
TGGTATC801.8153514E-938.401272
GAACGGA400.001889351936.80356667
GTATCAA9250.035.445571
TGCAACA701.0563126E-634.1344642
AGAACGG450.00351604432.42528566
ATCAACG10150.032.2705883
TCAACGC10300.031.8006274
AACGCAG10550.031.377386
CAACGCA10450.031.344165
TACATAT553.4279755E-431.08615126
AGACAGG1001.5952537E-830.7379571
AACGGAT500.004607490330.68255268
ATACATA450.004830213330.3886425
Produced by FastQC (version 0.11.5)