FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651713
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36970.567274244951382No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTCCGCTGAAGACCACATGATGACTATTACTATCCCACAC30900.4741350870705357No Hit
CCCCTATCCTGGTCGTTTTTGATGACACCGACCGGCCCTCAGGGATCCCC29230.4485103105201216No Hit
GGATAGGGGCCTGGCCTGCCCTCTGTTGGTACCAGTGAACAATAAGGCTT24230.3717894226446304No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19340.2967563943024No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18740.287549887757341No Hit
GTAATATACTCCCATATCGCCGTCTTTCGGGCTGTCCATTTGCAGGAACA18330.28125877495155077No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTC16980.2605441352251681No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16110.24719470073483268No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14650.22479220147518922No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14370.2204958317541617No Hit
GTACCAACAGAGGGCAGGCCAGGCCCCTATCCTGGTCGTTTTTGATGACA14330.2198820646511578No Hit
GCCATGGCCTGGACCATTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGATTCTCTGACCTCGTATGTCCTGACT13970.21435816072412242No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG13860.2126703011908616No Hit
GGCTTACCTGTGGGGGAACCGACATTGGAAGCCTTATTGTTCACTGGTAC13820.21205653408785768No Hit
CTGTTGGTACCAGTGAACAATAAGGCTTCCAATGTCGGTTCCCCCACAGGTAAGCCTGGCCGTCTGTCCTGGGGC13740.2108289998818498No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13010.19962775025202814No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12790.1962520311855065No Hit
GCCCTCAGGGATCCCCGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG12620.19364352099773982No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12340.18934715127671228No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCATTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGATTCTCTGA12270.18827305884645543No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11980.18382324734967692No Hit
GGGCAGGCCAGGCCCCTATCCTGGTCGTTTTTGATGACACCGACCGGCCC11930.18305603847092203No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11730.17998720295590237No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11620.17829934342264156No Hit
GTAATAGTCATCATGTGGTCTTCAGCGGAGGGACCAAGCTGACCGTCCTC11100.1703203710835905No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11010.16893939510183165No Hit
TTACTATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGGCTTCGAC10970.16832562799882772No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10650.16341549117479626No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG10620.16295516584754333No Hit
ATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGGCTTCGACCCCGG10470.1606535392112786No Hit
GACTATTACTATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGGCTTCGACCCCGGTGATGGTCAGGATGG10450.16034665565977663No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10350.1588122379022668No Hit
CCTCTCTCACTGCACAGATTCTCTGACCTCGTATGTCCTGACTCAGCCAC10350.1588122379022668No Hit
CCCCAGGACAGACGGCCAGGCTTACCTGTGGGGGAACCGACATTGGAAGC10340.15865879612651582No Hit
GGCCAGGCCCCTATCCTGGTCGTTTTTGATGACACCGACCGGCCCTCAGG10260.15743126192050796No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10250.15727782014475697No Hit
CGTATGTCCTGACTCAGCCACCCTCGGTCTCTGTGGCCCCAGGACAGACG10200.15651061126600205No Hit
GTATATTACTGTGCGAGAGAATATAGCAGTTCGTCGGATGGTTTTGATCT9970.15298145042372946No Hit
CTGTGGCCCCAGGACAGACGGCCAGGCTTACCTGTGGGGGAACCGACATT9960.1528280086479785No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9770.1499126149087098No Hit
GGATGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCGGGGATCCCTGAGGGCCGGTCGGTGTCATCAAAAA9730.14929884780570588No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9640.14791787182394706No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCATT9510.14592312873918428No Hit
CCCTCGGTCTCTGTGGCCCCAGGACAGACGGCCAGGCTTACCTGTGGGGGAACCGACATTGGAAGCCTTATTGTT9420.14454215275742544No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9240.14178020079390774No Hit
GTAATAATCGGCCTCATCCCCGGCTTCGACCCCGGTGATGGTCAGGATGG9040.1387113652788881No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8960.13748383107288026No Hit
GCCTTATTGTTCACTGGTACCAACAGAGGGCAGGCCAGGCCCCTATCCTGGTCGTTTTTGATGACACCGACCGGC8770.13456843733361157No Hit
ACAGAGGGCAGGCCAGGCCCCTATCCTGGTCGTTTTTGATGACACCGACCGGCCCTCAGGGATCCCCGAGCGATT8720.13380122845485665No Hit
ATCCTGACCATCACCGGGGTCGAAGCCGGGGATGAGGCCGATTATTACTGTCAGGTGTGGGATAGTAATAGTCAT8670.13303401957610175No Hit
CGGCCATCCTGACCATCACCGGGGTCGAAGCCGGGGATGAGGCCGATTAT8650.1327271360245998No Hit
GGCCTGGACCATTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGATTCTCTGACCTCGTATGTCCTGACTCAGC8640.1325736942488488No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8450.12965830050958013No Hit
GGATAGTAATAGTCATCATGTGGTCTTCAGCGGAGGGACCAAGCTGACCGTCCTCGGTCAGCCCAAGGCTGCCCC8400.12889109163082524No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT8320.12766355742481736No Hit
CTATTACTATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGGCTTC8170.12536193078855262No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCATTCTCCTCCTCGGC8140.12490160546129968No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8110.12444128013404673No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCATTCTCCTCCTCGG8080.1239809548067938No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG7950.12198621172203103No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC7910.12137244461902709No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7860.12060523574027218No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7820.11999146863726826No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGC7810.11983802686151726No Hit
GACCGAGGACGGTCAGCTTGGTCCCTCCGCTGAAGACCACATGATGACTA7790.11953114331001531No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCATTCTCCTCCTCGGCCTCCTCTC7730.11861049265550941No Hit
GTCCTGACTCAGCCACCCTCGGTCTCTGTGGCCCCAGGACAGACGGCCAG7710.11830360910400743No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA7500.11508133181323679No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7320.11231937984971913No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC7120.10925054433469947No Hit
TCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG7110.1090971025589485No Hit
ATTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGATTCTCTGACCTCGTATGTCCTGACTCAGCCACCCTCGGT7080.10863677723169554No Hit
CCTGAGGGCCGGTCGGTGTCATCAAAAACGACCAGGATAGGGGCCTGGCC7030.10786956835294065No Hit
GTGTGGGATAGTAATAGTCATCATGTGGTCTTCAGCGGAGGGACCAAGCTGACCGTCCTCGGTCAGCCCAAGG7020.10771612657718965No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7020.10771612657718965No Hit
GATTATTACTGTCAGGTGTGGGATAGTAATAGTCATCATGTGGTCTTCAG7010.10756268480143867No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTCCGCTGAAGACCACATGATGA6960.10679547592268375No Hit
TAGTAATAGTCATCATGTGGTCTTCAGCGGAGGGACCAAGCTGACCGTCC6850.10510761638942295No Hit
GTACTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC6790.10418696573491705No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6770.1038800821834151No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC6770.1038800821834151No Hit
CCTGTGGGGGAACCGACATTGGAAGCCTTATTGTTCACTGGTACCAACAG6700.1028059897531582No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA6650.10203878087440331No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT6580.10096468844414642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC707.2759576E-1249.36976241
GGTATCA5350.045.9985431
TCTGGAG5500.041.30991770
GTGGTAT1250.041.015371
AAAGGGG957.6397555E-1140.0316568
ATCTCCG955.456968E-1239.55801410
ATCTCGT801.6843842E-938.7044537
GTATCAA14150.038.6481671
TCTCCGA1051.8189894E-1135.7905811
TGGTATC1550.035.2657972
TATCAAC15750.034.706022
GTATGCC1102.7284841E-1134.6071842
TATACAC1400.034.1611183
ATCAACG16750.032.835463
CAACGCA17000.032.7569965
CTTATAC1155.2750693E-1132.693411
CCCACGA1155.2750693E-1132.67835619
AATACGT652.4806164E-531.53575910
TATCGCC3900.031.5333415
ATGCCGT1101.0004442E-931.52723744
Produced by FastQC (version 0.11.5)