FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651713
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32310.49577037745142416No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTCCGCTGAAGACCACATGATGACTATTACTATCCCACAC28250.43347301649652537No Hit
CCCCTATCCTGGTCGTTTTTGATGACACCGACCGGCCCTCAGGGATCCCC27900.4281025543452409No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20190.30979894524123347No Hit
GGATAGGGGCCTGGCCTGCCCTCTGTTGGTACCAGTGAACAATAAGGCTT19370.29721671962965296No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18040.27680896345477224No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16220.24888256026809347No Hit
GTAATATACTCCCATATCGCCGTCTTTCGGGCTGTCCATTTGCAGGAACA15890.24381898166831104No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTC15420.23660721820801486No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14320.21972862287540684No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG13980.21451160249987342No Hit
GCCATGGCCTGGACCATTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGATTCTCTGACCTCGTATGTCCTGACT13530.20760672259107918No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13500.20714639726382625No Hit
GTACCAACAGAGGGCAGGCCAGGCCCCTATCCTGGTCGTTTTTGATGACA13410.20576542128206743No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13270.2036172364215537No Hit
GGCTTACCTGTGGGGGAACCGACATTGGAAGCCTTATTGTTCACTGGTAC13130.2014690515610399No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12940.19855365782177123No Hit
CTGTTGGTACCAGTGAACAATAAGGCTTCCAATGTCGGTTCCCCCACAGGTAAGCCTGGCCGTCTGTCCTGGGGC12760.19579170585825356No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCATTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGATTCTCTGA12180.18689208286469658No Hit
GTAATAGTCATCATGTGGTCTTCAGCGGAGGGACCAAGCTGACCGTCCTC12100.18566454865868873No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12050.1848973397799338No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12000.18413013090117888No Hit
GGGCAGGCCAGGCCCCTATCCTGGTCGTTTTTGATGACACCGACCGGCCC11990.18397668912542792No Hit
CCTCTCTCACTGCACAGATTCTCTGACCTCGTATGTCCTGACTCAGCCAC11440.1755373914591239No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11410.17507706613187093No Hit
CGTATGTCCTGACTCAGCCACCCTCGGTCTCTGTGGCCCCAGGACAGACG11330.17384953192586305No Hit
GCCCTCAGGGATCCCCGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG11110.17047381285934146No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11000.16878595332608065No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10810.165870559586812No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCATT10650.16341549117479626No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10540.16172763164153545No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10380.15927256322951974No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG10180.1562037277145001No Hit
TTACTATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGGCTTCGAC9910.15206079976922357No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9790.1502194984602118No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9690.14868508070270195No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9610.1474575464966941No Hit
CTGTGGCCCCAGGACAGACGGCCAGGCTTACCTGTGGGGGAACCGACATT9550.1465368958421882No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCATTCTCCTCCTCGG9210.1413198754666548No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9120.13993889948489596No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCATTCTCCTCCTCGGC9110.13978545770914497No Hit
GGCCAGGCCCCTATCCTGGTCGTTTTTGATGACACCGACCGGCCCTCAGG9060.13901824883039005No Hit
GACTATTACTATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGGCTTCGACCCCGGTGATGGTCAGGATGG9060.13901824883039005No Hit
GTATATTACTGTGCGAGAGAATATAGCAGTTCGTCGGATGGTTTTGATCT8990.1379441564001332No Hit
GTCCTGACTCAGCCACCCTCGGTCTCTGTGGCCCCAGGACAGACGGCCAG8940.13717694752137827No Hit
GCCTTATTGTTCACTGGTACCAACAGAGGGCAGGCCAGGCCCCTATCCTGGTCGTTTTTGATGACACCGACCGGC8910.13671662219412534No Hit
CCCCAGGACAGACGGCCAGGCTTACCTGTGGGGGAACCGACATTGGAAGC8780.13472187910936256No Hit
GGATGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCGGGGATCCCTGAGGGCCGGTCGGTGTCATCAAAAA8780.13472187910936256No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCATTCTCCTCCTCGGCCTCCTCTC8760.13441499555786057No Hit
CCCTCGGTCTCTGTGGCCCCAGGACAGACGGCCAGGCTTACCTGTGGGGGAACCGACATTGGAAGCCTTATTGTT8760.13441499555786057No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT8730.13395467023060764No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8370.12843076630357228No Hit
CGGCCATCCTGACCATCACCGGGGTCGAAGCCGGGGATGAGGCCGATTAT8330.12781699920056835No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8310.1275101156490664No Hit
ATCCTGACCATCACCGGGGTCGAAGCCGGGGATGAGGCCGATTATTACTGTCAGGTGTGGGATAGTAATAGTCAT7960.12213965349778198No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7940.12183276994628003No Hit
GGATAGTAATAGTCATCATGTGGTCTTCAGCGGAGGGACCAAGCTGACCGTCCTCGGTCAGCCCAAGGCTGCCCC7920.12152588639477806No Hit
ACAGAGGGCAGGCCAGGCCCCTATCCTGGTCGTTTTTGATGACACCGACCGGCCCTCAGGGATCCCCGAGCGATT7900.12121900284327611No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT7900.12121900284327611No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7820.11999146863726826No Hit
GGCCTGGACCATTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGATTCTCTGACCTCGTATGTCCTGACTCAGC7810.11983802686151726No Hit
ATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGGCTTCGACCCCGG7730.11861049265550941No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7640.11722951667375056No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7600.11661574957074664No Hit
GGCCTGGACCATTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGATTCTC7560.11600198246774271No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGC7550.11584854069199171No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7470.11462100648598386No Hit
GGACCATTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGATTCTCTGACCTCGTATGTCCTGACTCAGCCACCCT7410.11370035583147797No Hit
GTGTGGGATAGTAATAGTCATCATGTGGTCTTCAGCGGAGGGACCAAGCTGACCGTCCTCGGTCAGCCCAAGG7370.11308658872847405No Hit
GTAATAATCGGCCTCATCCCCGGCTTCGACCCCGGTGATGGTCAGGATGG7300.11201249629821716No Hit
GGTACCAACAGAGGGCAGGCCAGGCCCCTATCCTGGTCGTTTTTGATGAC7270.11155217097096422No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7240.11109184564371127No Hit
CTATTACTATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGGCTTC7110.1090971025589485No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC7060.10832989368019358No Hit
ATTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGATTCTCTGACCTCGTATGTCCTGACTCAGCCACCCTCGGT7060.10832989368019358No Hit
GACCGAGGACGGTCAGCTTGGTCCCTCCGCTGAAGACCACATGATGACTA7030.10786956835294065No Hit
TCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG6830.10480073283792098No Hit
TAGTAATAGTCATCATGTGGTCTTCAGCGGAGGGACCAAGCTGACCGTCC6710.1029594315289092No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG6710.1029594315289092No Hit
GTACTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC6670.10234566442590526No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT6590.1011181302198974No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAAC6580.10096468844414642No Hit
GCTTGGTCCCTCCGCTGAAGACCACATGATGACTATTACTATCCCACACCTGACAGTAATAATCGGCCTCATCCC6560.10065780489264446No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTCCGCTGAAGACCACATGATGA6520.10004403778964054No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTCG100.009219136132.7977970
TACGTAT400.00856090449.7991770
AATCGAG400.00856090449.7991770
GTTCTAG507.469316E-847.850251
GGTATCA5550.044.9558371
GGTACGT358.3475607E-443.4579768
TCTGGAG5000.041.16731670
TGGTATC1550.039.645772
GTACGTA450.001615819337.99609469
GTATCAA13950.036.7513431
GTGGTAT1700.036.1892661
GCGTAGA1201.7428143E-533.1994570
AGGTAAG10800.031.35503670
TATCAAC15900.031.3481452
ATCAACG16450.030.507563
TCAACGC16850.029.5807384
CAACGCA17000.029.3219835
TGGTACG500.00584598129.22663567
TAATTCG605.6224E-428.57008232
AACGCAG17400.028.4560626
Produced by FastQC (version 0.11.5)