FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708990
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26640.375745779207041No Hit
ATATAAGAGTTCTCCGTACACTTTTGGCCAGGGGACCAGCCTGGAAATCAGACGAACTGTGGCTGCACCAT21290.3002863227972186No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21230.2994400485197253No Hit
CTCCTAGACTACTCATTTACGGGGCGTCTACCCGGGCTTCCGGGGTCCCT20590.29041312289312965No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18140.2558569232288184No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17930.25289496325759175No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16850.23766202626271174No Hit
CTCTTATATTGGTGACAGAAATAAACTGCCACATCTCCAGCCTGCAGGCCGCCAATGGTGAGAGTGAAATCTGTC16800.23695679769813396No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16470.23230228917192064No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16280.22962242062652505No Hit
GTCTAGGAGGCTGTCCTGGTTTGAACTGGTACCAAGCTAAGCTGTGCGCAGTGTCGGAGAAGTCCAGAAGACT15720.22172386070325392No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15110.21312007221540502No Hit
CTCCGACACTGCGCACAGCTTAGCTTGGTACCAGTTCAAACCAGGACAGC14010.19760504379469387No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12420.17517877544112045No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12270.17306308974738713No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11910.1679854440824271No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11690.1648824383982849No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11610.16375407269496042No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11210.1581122441783382No Hit
GCTTAGCTTGGTACCAGTTCAAACCAGGACAGCCTCCTAGACTACTCATT11160.15740701561376041No Hit
CTTTTGGCCAGGGGACCAGCCTGGAAATCAGACGAACTGTGGCTGCACCA10920.15402191850378708No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10430.14711067857092483No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10260.14471290145136037No Hit
CTTCTGGACTTCTCCGACACTGCGCACAGCTTAGCTTGGTACCAGTTCAA9960.14048153006389372No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9750.13751957009266702No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT9510.13413447298269368No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC9110.12849264446607145No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9040.12750532447566257No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9040.12750532447566257No Hit
GTGTCGGAGAAGTCCAGAAGACTCTGGCTGGCCTTGCAGTTGACGGTGGC8860.12496650164318256No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8740.12327395308819589No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8690.1225687245236181No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT8670.12228663309778699No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8610.12144035882029366No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8400.11847839884906698No Hit
GTTCAAACCAGGACAGCCTCCTAGACTACTCATTTACGGGGCGTCTACCCGGGCTTCCGGGGTCCCTGACAGATT8100.11424702746160031No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8020.11311866175827585No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7900.11142611320328918No Hit
GGCCAAAAGTGTACGGAGAACTCTTATATTGGTGACAGAAATAAACTGCC7860.11086193035162696No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7850.1107208846387114No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7840.11057983892579584No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7780.10973356464830251No Hit
GATTTCACTCTCACCATTGGCGGCCTGCAGGCTGGAGATGTGGCAGTTTATTTCTGTCACCAATATAAGAGTTCT7750.10931042750955584No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC7720.10888729037080917No Hit
GAGTGAAATCTGTCCCAGAGCCGGTGCCACGGAATCTGTCAGGGACCCCG7680.10832310751914696No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7670.10818206180623141No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7590.10705369610290695No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCGTCAACTGCAAGGCCAGCCAGAGTCTTCTGGA7500.10578428468666695No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7470.10536114754792028No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT7460.10522010183500471No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7430.10479696469625807No Hit
GTGCAGCCACAGTTCGTCTGATTTCCAGGCTGGTCCCCTGGCCAAAAGTG7410.10451487327042695No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC7410.10451487327042695No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7400.1043738275575114No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7300.10296337042835584No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7270.10254023328960916No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7210.10169395901211582No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG7180.10127082187336915No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG7160.10098873044753806No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGTA200.007777189451.0295530
GTGGTAT2450.047.1110341
TGGTATC2600.044.3523862
GGTATCA7800.042.2169571
GTATCAA21100.040.222341
ACCGTAC1950.040.0634527
TCGTATG1950.038.91526840
TAGATCT2050.038.34203334
CGTACTA2050.038.1495529
GCCGTCT2100.038.14198746
GTATGCC2150.037.0190642
CGTATGC2150.036.9658641
CTAGATC2150.036.5197633
TACTAGA2150.036.4266731
GTACTAG2150.036.3931730
GACCGTA2150.036.32122826
GCGATTT901.5632185E-435.5323970
ACTAGAT2250.034.84965532
TATCAAC24600.034.7437672
CTACTAG502.0457705E-433.947661
Produced by FastQC (version 0.11.5)