FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708990
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAGAGTTCTCCGTACACTTTTGGCCAGGGGACCAGCCTGGAAATCAGACGAACTGTGGCTGCACCAT21720.30635128845258747No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21610.30479978561051635No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20420.28801534577356525No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19490.2748980944724185No Hit
CTCCTAGACTACTCATTTACGGGGCGTCTACCCGGGCTTCCGGGGTCCCT19450.27433391162075627No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18740.2643196660037518No Hit
CTCTTATATTGGTGACAGAAATAAACTGCCACATCTCCAGCCTGCAGGCCGCCAATGGTGAGAGTGAAATCTGTC16780.23667470627230283No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15770.2224290892678317No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15680.22115967785159169No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14660.20677301513420498No Hit
GTCTAGGAGGCTGTCCTGGTTTGAACTGGTACCAAGCTAAGCTGTGCGCAGTGTCGGAGAAGTCCAGAAGACT14100.19887445521093386No Hit
CTCCGACACTGCGCACAGCTTAGCTTGGTACCAGTTCAAACCAGGACAGC13960.19689981523011607No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12920.18223106108689827No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12640.17828178112526272No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12430.17531982115403602No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11930.16826753550825824No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11840.16699812409201822No Hit
GCTTAGCTTGGTACCAGTTCAAACCAGGACAGCCTCCTAGACTACTCATT11100.15656074133626707No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10960.15458610135544928No Hit
CTTTTGGCCAGGGGACCAGCCTGGAAATCAGACGAACTGTGGCTGCACCA10500.14809799856133374No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10320.1455591757288537No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10240.1444308100255293No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9660.13625015867642704No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9580.13512179297310256No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9560.13483970154727148No Hit
CTTCTGGACTTCTCCGACACTGCGCACAGCTTAGCTTGGTACCAGTTCAA9370.1321598330018759No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8590.12115826739446255No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC8570.12087617596863143No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8530.1203119931169692No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8530.1203119931169692No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8320.11735003314574254No Hit
GTTCAAACCAGGACAGCCTCCTAGACTACTCATTTACGGGGCGTCTACCCGGGCTTCCGGGGTCCCTGACAGATT8310.11720898743282698No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7850.1107208846387114No Hit
AACCAGGACAGCCTCCTAGACTACTCATTTACGGGGCGTCTACCCGGGCT7650.10789997038040029No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7640.10775892466748473No Hit
GGCCAAAAGTGTACGGAGAACTCTTATATTGGTGACAGAAATAAACTGCC7630.10761787895456917No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7580.10691265038999138No Hit
GAGTGAAATCTGTCCCAGAGCCGGTGCCACGGAATCTGTCAGGGACCCCG7500.10578428468666695No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7450.10507905612208916No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7410.10451487327042695No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7360.10380964470584916No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7300.10296337042835584No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7280.10268127900252473No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7270.10254023328960916No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT7240.1021170961508625No Hit
GATTTCACTCTCACCATTGGCGGCCTGCAGGCTGGAGATGTGGCAGTTTATTTCTGTCACCAATATAAGAGTTCT7170.1011297761604536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8350.044.1870731
TGGTATC2450.044.1869432
GTGGTAT2600.041.661211
GTATCAA22850.041.034471
GTACGGT350.00148316338.666314
TATTAGA853.592504E-935.8206672
TATCAAC27300.034.2023932
ATCAACG28550.032.4610563
TCAACGC28800.032.1792764
AACGCAG28850.032.1280336
CAACGCA29400.031.4097275
CGCAGAG30550.030.5616868
AGAGTAC30450.030.55095911
CAGAGTA30650.030.46197510
GATCTAG450.005072232330.0886521
GCAGAGT31700.029.666419
GACAGGT2300.029.4345511
ATAGTAC606.085903E-428.1862243
ATTAGAG1104.434696E-827.673753
ACGCAGA34200.027.3988977
Produced by FastQC (version 0.11.5)