FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1318924
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44930.34065647452013914No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42480.3220807264103163No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAGCA37640.28538414647091115No Hit
CTCCCAGACTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCA30660.23246221920292603No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29290.22207496413743322No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT29230.22162004785719266No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28460.21578195559410548No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGATT26860.20365085478769054No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26660.20213446718688868No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT25000.18954845010023322No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG24950.18916935320003275No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAATTACTTAGCCTGGTA24470.18553002295810828No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23360.17711407177365793No Hit
GCCCTATATTATTGTACCGCGCCAAGTCCGGATTACTATGATAGTAGTGG23350.17703825239361784No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAATTACTTAGCCTGGTACCAACAGAGACCTGGCCAGACTCCCAGACT23290.17658333611337726No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23210.1759767810730565No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23170.17567350355289615No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23140.17544604541277586No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22500.1705936050902099No Hit
GTACCAACAGAGACCTGGCCAGACTCCCAGACTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGC21470.16278420894608028No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20850.1580834073835945No Hit
GTATTCAGGCTGTTCATTTGCAGATACACCGTGTTTTCTGAATCATCTCT20770.15747685234327374No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20740.15724939420315348No Hit
GTCTGGGAGTCTGGCCAGGTCTCTGTTGGTACCAGGCTAAGTAATTGCTA20600.15618792288259217No Hit
GTTTATTACTGTCAGCAGCGTAGCCAAGCTTTCGGCCCTGGGACCAAAGTGAATTTCAGACGAACTGTGGCTGCA20470.15520227094207095No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20440.1549748128019507No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20290.1538375221013493No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT19680.1492125399189036No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19510.14792361045822203No Hit
TTATTGTACCGCGCCAAGTCCGGATTACTATGATAGTAGTGGCTGGACTC19500.1478477910781819No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19300.14633140347738005No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19280.14617976471729985No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGAAATTCACTTTGGTCCCAGGGCCGAAAGCTTGGCTACGCTG18900.14329862827577633No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC18700.14178224067497447No Hit
GTGCAGCCACAGTTCGTCTGAAATTCACTTTGGTCCCAGGGCCGAAAGCTTGGCTACGCTGCTGACAGTAATAAA18610.14109986625461363No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18360.13920438175361127No Hit
GTAATAAACTGCAAAATCTTCAGGCTCCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA18330.13897692361349098No Hit
ATATAGGGCTGTGTCCTCGGTATTCAGGCTGTTCATTTGCAGATACACCG18120.13738471663264903No Hit
GACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGATTTTGCAGTTTA18060.13692980035240848No Hit
GTCAGAGTGTTAGCAATTACTTAGCCTGGTACCAACAGAGACCTGGCCAGACTCCCAGACTCCTCATCTATGATG18010.13655070345220802No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC17790.13488267709132598No Hit
GTACAATAATATAGGGCTGTGTCCTCGGTATTCAGGCTGTTCATTTGCAGATACACCGTGTTTTCTGAATCATCT17550.13306301197036371No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17440.1322289987899227No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17360.13162244374960194No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA17150.13003023676876No Hit
ATATTATTGTACCGCGCCAAGTCCGGATTACTATGATAGTAGTGGCTGGA16740.12692164218711616No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16690.1265425452869157No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16610.12593599024659494No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16590.12578435148651476No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16460.12479869954599355No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16340.12388886698551244No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT16290.12350977008531196No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16110.12214502124459028No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15960.12100773054398888No Hit
CTGTTGGTACCAGGCTAAGTAATTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGA15920.12070445302382851No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15910.12062863364378842No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15750.11941552356314693No Hit
GCCTGAATACCGAGGACACAGCCCTATATTATTGTACCGCGCCAAGTCCGGATTACTATGATAGTAGTGGCTGGA15740.11933970418310684No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15410.11683766464178375No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT15190.11516963828090171No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15190.11516963828090171No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15100.11448726386054087No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT14880.11281923749965882No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14840.11251595997949844No Hit
GCCACAGTTCGTCTGAAATTCACTTTGGTCCCAGGGCCGAAAGCTTGGCT14810.11228850183937816No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14730.11168194679905741No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA14720.11160612741901732No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14640.11099957237869658No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14510.11001392043817537No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14390.10910408787769424No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT14390.10910408787769424No Hit
ACAGAGACCTGGCCAGACTCCCAGACTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT14310.10849753283737348No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14310.10849753283737348No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14170.10743606151681219No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA14090.10682950647649145No Hit
GCCTTGGGATTCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAGA14020.1062987708162108No Hit
CTGCTACTCTGGCTCCCAGAAAGCACCGGAGAAGTTGTGTTGACACAGTC14000.1061471320561306No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13880.10523729949564947No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13880.10523729949564947No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13430.10182542739384527No Hit
GTCTGGGGGAGGCCTGGTAAAGCCTGGGGGGTCCCTTAGAATTTCCTGTGCAGCCTCTGGATTCACTTTCAGTAA13250.10046067855312361No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAG200.006908484352.5838652
CGGACGA400.00936943648.68020270
TCGTATG2150.048.20656640
GTGGTAT3150.047.7506521
CGTATGC2200.047.16700741
TGGTATC3300.046.600172
GGTATCA11650.044.895481
ATCTCGT2000.043.0563437
CACGAGA2500.042.3831221
ATGCCGT2550.042.18551344
CGAGACA2600.040.7684623
GTATGCC2650.040.50317442
CTTATAC2800.040.2896081
ATCTCCG2550.040.20559310
CCGTCTT2600.040.1632947
TGCTTGA2750.039.66255655
GCCGTCT2650.039.3674946
GGATCTC2200.039.08686435
ACGAGAC2750.038.53303522
CCCACGA2850.038.3745619
Produced by FastQC (version 0.11.5)