FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1318924
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43570.33034503883468647No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35960.27264649062417545No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAGCA34530.2618043192784421No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33950.2574067952361167No Hit
CTCCCAGACTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCA28510.21616105249430595No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28150.2134315548128626No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT27260.20668362998929432No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGATT26080.19773694314456328No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26020.19728202686432272No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25230.19129229584115537No Hit
GCCCTATATTATTGTACCGCGCCAAGTCCGGATTACTATGATAGTAGTGG23880.18105667953574278No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAATTACTTAGCCTGGTA23820.1806017632555022No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23810.1805259438754621No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23450.17779644619401877No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT23240.1762042392131768No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAATTACTTAGCCTGGTACCAACAGAGACCTGGCCAGACTCCCAGACT22160.16801574616884674No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21170.1605096275448775No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20920.15861414304387517No Hit
GTTTATTACTGTCAGCAGCGTAGCCAAGCTTTCGGCCCTGGGACCAAAGTGAATTTCAGACGAACTGTGGCTGCA20800.15770431048339403No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20650.15656701978279264No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20390.15459571590175022No Hit
GTCTGGGAGTCTGGCCAGGTCTCTGTTGGTACCAGGCTAAGTAATTGCTA20240.1534584252011488No Hit
GTACCAACAGAGACCTGGCCAGACTCCCAGACTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGC19720.14951581743906397No Hit
GTAATAAACTGCAAAATCTTCAGGCTCCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19520.14799942983826211No Hit
TTATTGTACCGCGCCAAGTCCGGATTACTATGATAGTAGTGGCTGGACTC19370.1468621391376607No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19300.14633140347738005No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19260.14602812595721967No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT19210.1456490290570192No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18870.14307117013565604No Hit
GTGCAGCCACAGTTCGTCTGAAATTCACTTTGGTCCCAGGGCCGAAAGCTTGGCTACGCTGCTGACAGTAATAAA18530.14049331121429284No Hit
GTATTCAGGCTGTTCATTTGCAGATACACCGTGTTTTCTGAATCATCTCT18510.14034167245421267No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18250.13837036857317025No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18150.13761217477276932No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC18030.1367023422122882No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17870.1354892321316467No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17840.13526177399152642No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17810.13503431585140613No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17350.13154662436956185No Hit
GACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGATTTTGCAGTTTA17350.13154662436956185No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGAAATTCACTTTGGTCCCAGGGCCGAAAGCTTGGCTACGCTG17280.13101588870928121No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17260.130864249949201No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17180.13025769490888028No Hit
GTCAGAGTGTTAGCAATTACTTAGCCTGGTACCAACAGAGACCTGGCCAGACTCCCAGACTCCTCATCTATGATG16760.12707328094719636No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16680.1264667259068756No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC16640.12616344838671523No Hit
ATATTATTGTACCGCGCCAAGTCCGGATTACTATGATAGTAGTGGCTGGA16290.12350977008531196No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16290.12350977008531196No Hit
CTGTTGGTACCAGGCTAAGTAATTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGA16010.12138682744418935No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15960.12100773054398888No Hit
ATATAGGGCTGTGTCCTCGGTATTCAGGCTGTTCATTTGCAGATACACCG15890.12047699488370824No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15670.11880896852282617No Hit
GTACAATAATATAGGGCTGTGTCCTCGGTATTCAGGCTGTTCATTTGCAGATACACCGTGTTTTCTGAATCATCT15400.11676184526174366No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT15330.11623110960146302No Hit
GCCTGAATACCGAGGACACAGCCCTATATTATTGTACCGCGCCAAGTCCGGATTACTATGATAGTAGTGGCTGGA15320.11615529022142293No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA15260.11570037394118236No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15200.1152454576609418No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14610.1107721142385763No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14450.1095590041579348No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14280.10827007469725322No Hit
CTGCTACTCTGGCTCCCAGAAAGCACCGGAGAAGTTGTGTTGACACAGTC14270.10819425531721312No Hit
GCCACAGTTCGTCTGAAATTCACTTTGGTCCCAGGGCCGAAAGCTTGGCT14120.10705696461661172No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14090.10682950647649145No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14050.10652622895633106No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14020.1062987708162108No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA14010.10622295143617069No Hit
GTCTGGGGGAGGCCTGGTAAAGCCTGGGGGGTCCCTTAGAATTTCCTGTGCAGCCTCTGGATTCACTTTCAGTAA13980.1059954932960504No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13950.10576803515593013No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13940.10569221577589004No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC13930.10561639639584995No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13890.10531311887568959No Hit
ACAGAGACCTGGCCAGACTCCCAGACTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT13860.10508566073556931No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13780.10447910569524854No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT13660.10356927313476742No Hit
GCCTTGGGATTCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAGA13600.10311435685452688No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA13480.10220452429404575No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC13260.1005364979331637No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)