FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences829726
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTAATACTCAGCCTCATCCTCAGACTGGAGGTTGGCGATGGTGAGGTAGCGGTCAGCCCCAGAGCTGGAGC34880.4203797398177229No Hit
GTATTACTGTGAGACCTGGGGCAGTAACATTGTGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC34100.4109790460947349No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCAC33450.4031451346589115No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31910.3845847906417299No Hit
CTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAGCTCACCTGCACT31720.3822948780681815No Hit
GAGTATTACTGTGAGACCTGGGGCAGTAACATTGTGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG31580.3806075740666196No Hit
GCTCTGGGGAGTCTGCACCATGGCCTGGACCCCACTCCTCCTCCTCTTCCCTCTCCTCCTCCACTGCACAGGGT29400.3543338403280119No Hit
GTAATACTCAGCCTCATCCTCAGACTGGAGGTTGGCGATGGTGAGGTAGC28620.34493314660502383No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27370.3298679323053635No Hit
GGGTCTCTCTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAGCTCAC26950.32480602030067757No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACAATGTTACTGCCCCAGGTCTCACAGTAATACTCAG26100.31456167457690853No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23810.2869622019799307No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC22120.2665940322467899No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21180.2552649910934453No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20670.2491183836591839No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20350.24526168879847082No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18470.22260360649178162No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17990.21681856420071205No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17840.2150107384847528No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17840.2150107384847528No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGGCTCCGCTCCCCTTGCT17780.2142876081983691No Hit
GCACAGGGTCTCTCTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAG17450.21031039162325874No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT16660.2007891761858734No Hit
CCTGCACTCTGAGCAGTGGGCACAGTAACTACATCATCGCATGGCATCAG16450.19825822018353045No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16250.1958477858955848No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16030.1931963081788446No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15860.19114743903409076No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15490.1866881356013913No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15270.1840366578846511No Hit
CACCTGCACTCTGAGCAGTGGGCACAGTAACTACATCATCGCATGGCATC15130.18234935388308912No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14980.18054152816712987No Hit
CAGTAACTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTC14830.17873370245117062No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14700.17716692016400595No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14650.17656431159201955No Hit
GTAACTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTCGG14630.17632326816322497No Hit
CCACTGCACAGGGTCTCTCTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGT14260.1718639647305255No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13830.16668153101144234No Hit
GTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAGCTCACCTGCACTCTGAGCAGTGGG13570.16354796643711297No Hit
CCTCCAGTCTGAGGATGAGGCTGAGTATTACTGTGAGACCTGGGGCAGTAACATTGTGATATTCGGCGGAGGGAC13540.16318640129392115No Hit
GCTCTGGGGCTGACCGCTACCTCACCATCGCCAACCTCCAGTCTGAGGATGAGGCTGAGTATTACTGTGAGAC12780.15402675099972762No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12720.15330362071334394No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12620.1520984035693711No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12590.15173683842617924No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT12580.15161631671178197No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACAATGTTACTGCCCCAGGTCTCACAGTAATACTCAGC12480.15041109956780913No Hit
GGCCTGGACCCCACTCCTCCTCCTCTTCCCTCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCCTGTGCTGA12440.14992901271022No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATC12350.14884431728064446No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12330.1486032738518499No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12250.14763910013667164No Hit
GTATCAACGCAGAGTACGGGAGCAGAGCTCTGGGGAGTCTGCACCATGGCCTGGACCCCACTCCTCCTCCTCTTC12080.1455902309919178No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11890.14330031841836943No Hit
TCCTCAGACTGGAGGTTGGCGATGGTGAGGTAGCGGTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGGCT11810.14233614470319117No Hit
GTCTCTCTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAGCTCACCTG11600.13980518870084824No Hit
GTCTGCACCATGGCCTGGACCCCACTCCTCCTCCTCTTCCCTCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAG11220.13522536355375148No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG11100.13377910298098408No Hit
GACTGGAGGTTGGCGATGGTGAGGTAGCGGTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGGCTCCGCTC11090.13365858126658678No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC10960.1320917989794221No Hit
CAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAGCTCACCTGCACTCTGAGCAGTGGGCACAGTAA10910.1314891904074357No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC10570.12739145211792807No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10460.12606571325955798No Hit
GTTCTTGATGAACGTTGGAAGTGGTGGAACCTACAGCAAGGGGAGCGGAGCCCCTGATCGCTTCTCAGGCTCCAG10230.12329371382842047No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10190.12281162697083134No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10110.12184745325565308No Hit
CTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTCGGTTCTTGATGAACGTTGGAAGTGGTGGAAC10060.12124484468366666No Hit
CAATGTTACTGCCCCAGGTCTCACAGTAATACTCAGCCTCATCCTCAGAC10020.12076275782607751No Hit
CTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCCTGTGCTGACTCAATC9980.12028067096848838No Hit
CTACCTCACCATCGCCAACCTCCAGTCTGAGGATGAGGCTGAGTATTACT9970.12016014925409112No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9910.1194370189677074No Hit
GAGCTGGAGCCTGAGAAGCGATCAGGGGCTCCGCTCCCCTTGCTGTAGGT9880.11907545382451558No Hit
GTCTGAGGATGAGGCTGAGTATTACTGTGAGACCTGGGGCAGTAACATTGTGATATTCGGCGGAGGGACCAAG9790.11799075839494001No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9780.11787023668054274No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA9750.11750867153735088No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9730.11726762810855632No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACAATGTTA9640.11618293267898078No Hit
TCCCTGGGATCCTCGGTCAAGCTCACCTGCACTCTGAGCAGTGGGCACAGTAACTACATCATCGCATGGCATCAG9610.11582136753578891No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9600.11570084582139165No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG9550.11509823724940524No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9250.11148258581748674No Hit
GCTTGGTCCCTCCGCCGAATATCACAATGTTACTGCCCCAGGTCTCACAGTAATACTCAGCCTCATCCTCAGA9220.11112102067429488No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC9080.10943371667273294No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC9060.10919267324393837No Hit
GGGAAGGCCCCTCGGTTCTTGATGAACGTTGGAAGTGGTGGAACCTACAGCAAGGGGAGCGGAGCCCCTGATCG8810.1061796303840063No Hit
TCCCTCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCC8770.10569754352641716No Hit
CTCCAGCTCTGGGGCTGACCGCTACCTCACCATCGCCAACCTCCAGTCTG8690.1047333698112389No Hit
CTTCCAACGTTCATCAAGAACCGAGGGGCCTTCCCTGGCTGCTGCTGATGCCATGCGATGATGTAGTTACTGTGC8670.10449232638244434No Hit
CTCTGAGCAGTGGGCACAGTAACTACATCATCGCATGGCATCAGCAGCAG8660.10437180466804703No Hit
GAGTACGGGAGCAGAGCTCTGGGGAGTCTGCACCATGGCCTGGACCCCACTCCTCCTCCTCTTCCCTCTCCTCCT8560.1031665875240742No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8490.10232293552329323No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC8300.10003302294974487No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)