FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences829726
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTGAGACCTGGGGCAGTAACATTGTGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC34760.4189334792449556No Hit
CTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAGCTCACCTGCACT33310.4014578306573495No Hit
CAGTAATACTCAGCCTCATCCTCAGACTGGAGGTTGGCGATGGTGAGGTAGCGGTCAGCCCCAGAGCTGGAGC32540.39217765864875875No Hit
GAGTATTACTGTGAGACCTGGGGCAGTAACATTGTGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG32510.3918160935055669No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32400.3904903546471968No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31060.37434044491796087No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCAC28790.3469820157497776No Hit
GGGTCTCTCTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAGCTCAC28160.3393891477427488No Hit
GCTCTGGGGAGTCTGCACCATGGCCTGGACCCCACTCCTCCTCCTCTTCCCTCTCCTCCTCCACTGCACAGGGT27370.3298679323053635No Hit
GTAATACTCAGCCTCATCCTCAGACTGGAGGTTGGCGATGGTGAGGTAGC26680.3215519340119509No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26200.31576689172088135No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACAATGTTACTGCCCCAGGTCTCACAGTAATACTCAG23390.28190028997524486No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22520.2714149008226812No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC20730.24984151394556756No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20320.24490012365527897No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18980.22875021392604308No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18820.22682186649568653No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18800.22658082306689195No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18750.22597821449490554No Hit
GCACAGGGTCTCTCTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAG17850.21513126019915008No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17080.20585108819055928No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16650.20066865447147614No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15730.1895806567469261No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15320.1846392664566375No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15270.1840366578846511No Hit
CCTGCACTCTGAGCAGTGGGCACAGTAACTACATCATCGCATGGCATCAG15230.18355457102706196No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15160.18271091902628095No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGGCTCCGCTCCCCTTGCT15090.1818672670255No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14930.17993891959514347No Hit
CACCTGCACTCTGAGCAGTGGGCACAGTAACTACATCATCGCATGGCATC14620.1762027464488277No Hit
CAGTAACTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTC14360.17306918187449832No Hit
GTAACTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTCGG14330.17270761673130647No Hit
GTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAGCTCACCTGCACTCTGAGCAGTGGG14140.17041770415775812No Hit
CCACTGCACAGGGTCTCTCTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGT13990.16860987844179884No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13960.168248313298607No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13940.16800726986981243No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13640.16439161843789393No Hit
CCTCCAGTCTGAGGATGAGGCTGAGTATTACTGTGAGACCTGGGGCAGTAACATTGTGATATTCGGCGGAGGGAC13420.16174014072115372No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13310.1604144018627836No Hit
GCTCTGGGGCTGACCGCTACCTCACCATCGCCAACCTCCAGTCTGAGGATGAGGCTGAGTATTACTGTGAGAC12660.15258049042696023No Hit
GTATCAACGCAGAGTACGGGAGCAGAGCTCTGGGGAGTCTGCACCATGGCCTGGACCCCACTCCTCCTCCTCTTC12550.1512547515685901No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATC12430.14980849099582272No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12300.14824170870865802No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12020.14486710070553413No Hit
GTCTCTCTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAGCTCACCTG11730.1413719709880129No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT11620.1400462321296428No Hit
CAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAGCTCACCTGCACTCTGAGCAGTGGGCACAGTAA11560.1393231018432591No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11540.13908205841446453No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACAATGTTACTGCCCCAGGTCTCACAGTAATACTCAGC11310.13631005898332701No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11210.1351048418393542No Hit
TCCTCAGACTGGAGGTTGGCGATGGTGAGGTAGCGGTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGGCT11170.13462275498176507No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC11100.13377910298098408No Hit
GGCCTGGACCCCACTCCTCCTCCTCTTCCCTCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCCTGTGCTGA10990.132453364122614No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC10930.13173023383623028No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10820.13040449497786016No Hit
GACTGGAGGTTGGCGATGGTGAGGTAGCGGTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGGCTCCGCTC10780.12992240812027103No Hit
CTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCCTGTGCTGACTCAATC10580.12751197383232538No Hit
CTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTCGGTTCTTGATGAACGTTGGAAGTGGTGGAAC10550.12715040868913352No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10430.12570414811636613No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10420.12558362640196885No Hit
GTCTGCACCATGGCCTGGACCCCACTCCTCCTCCTCTTCCCTCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAG10260.12365527897161231No Hit
GTCTGAGGATGAGGCTGAGTATTACTGTGAGACCTGGGGCAGTAACATTGTGATATTCGGCGGAGGGACCAAG10240.12341423554281775No Hit
GTTCTTGATGAACGTTGGAAGTGGTGGAACCTACAGCAAGGGGAGCGGAGCCCCTGATCGCTTCTCAGGCTCCAG10200.12293214868522861No Hit
TCCCTCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCC10080.12148588811246122No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10010.12064223611168025No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACAATGTTA9910.1194370189677074No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC9750.11750867153735088No Hit
TCCCTGGGATCCTCGGTCAAGCTCACCTGCACTCTGAGCAGTGGGCACAGTAACTACATCATCGCATGGCATCAG9510.11461615039181611No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA9470.11413406353422698No Hit
GAGCTGGAGCCTGAGAAGCGATCAGGGGCTCCGCTCCCCTTGCTGTAGGT9410.11341093324784327No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9290.11196467267507587No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG9110.10979528181592477No Hit
CTACCTCACCATCGCCAACCTCCAGTCTGAGGATGAGGCTGAGTATTACT9060.10919267324393837No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8990.10834902124315737No Hit
CAATGTTACTGCCCCAGGTCTCACAGTAATACTCAGCCTCATCCTCAGAC8950.10786693438556824No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8940.10774641267117097No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8930.10762589095677368No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG8930.10762589095677368No Hit
CCTCTTCCCTCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTG8880.10702328238478727No Hit
CTTCCAACGTTCATCAAGAACCGAGGGGCCTTCCCTGGCTGCTGCTGATGCCATGCGATGATGTAGTTACTGTGC8850.10666171724159541No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8810.1061796303840063No Hit
GCTTGGTCCCTCCGCCGAATATCACAATGTTACTGCCCCAGGTCTCACAGTAATACTCAGCCTCATCCTCAGA8770.10569754352641716No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG8700.10485389152563616No Hit
GAGTACGGGAGCAGAGCTCTGGGGAGTCTGCACCATGGCCTGGACCCCACTCCTCCTCCTCTTCCCTCTCCTCCT8550.10304606580967693No Hit
CTCCAGCTCTGGGGCTGACCGCTACCTCACCATCGCCAACCTCCAGTCTG8510.1025639789520878No Hit
CTCCTCCTCCTCTTCCCTCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCCTGTGCTGACTCAATCATCCT8470.10208189209449867No Hit
CTCTGAGCAGTGGGCACAGTAACTACATCATCGCATGGCATCAGCAGCAG8420.10147928352251226No Hit
GGGAAGGCCCCTCGGTTCTTGATGAACGTTGGAAGTGGTGGAACCTACAGCAAGGGGAGCGGAGCCCCTGATCG8300.10003302294974487No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)