FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279629
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13800.49351104499175696No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA13360.4777759102239038No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12910.4616831587567813No Hit
GTATATTACTGTCAGCAGTATGGTAGGTCACCCATGTACACTTTTGGCCA12280.4391533067028098No Hit
GTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12260.438438073304271No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT11060.39552406939194434No Hit
ATATTACTGTCAGCAGTATGGTAGGTCACCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA10850.3880141187072872No Hit
GTATGGTAGGTCACCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT10030.3586895493671972No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9670.34581534819349924No Hit
CAGTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9570.3422391812008054No Hit
CATCTATGGTGCATCCAAGAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA9200.329007363327838No Hit
GTGTTAGCAGTACCTACTTAGCCTGGTACCAGCAGAAAACTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT9020.322570262740989No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7750.27715294193377654No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7640.27321915824181325No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA7590.2714310747454663No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGTTTTCTGCTGGTACCAGGCTAAGTAGGTACTGCTAACACTCTGA7560.27035822464765813No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGTACCTACTTAGCCTGGTACC7530.26928537454985No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7400.26463635745934794No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7190.2571264067746908No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6830.24425220560099276No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC6680.2388879551119519No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA6650.2378151050141437No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6620.23674225491633555No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6560.23459655472071925No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTCTT6540.23388132132218048No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6470.23137800442729473No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT6470.23137800442729473No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT6420.2295899209309478No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6400.228874687532409No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA6370.22780183743460083No Hit
GTCAGAGTGTTAGCAGTACCTACTTAGCCTGGTACCAGCAGAAAACTGGC6340.22672898733679267No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGTA6310.22565613723898453No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA5990.2142124028623641No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACATGGGTGA5950.21278193606528653No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5930.21206670266674774No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5760.2059872187791681No Hit
CCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC5700.20384151858355176No Hit
GGCCAAAAGTGTACATGGGTGACCTACCATACTGCTGACAGTAATATACTGCAAAATCTTCAGGCTCCAGTCTG5490.1963315678988946No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTATATTACTGTCAGCAGTATGGTAGGTCA5380.19239778420693132No Hit
GTACCTACTTAGCCTGGTACCAGCAGAAAACTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAAGAGGG5330.19060970071058436No Hit
GTACGGGAGAAGAGCTGCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTG5290.18917923391350683No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT5190.18560306692081294No Hit
GAGTGTTAGCAGTACCTACTTAGCCTGGTACCAGCAGAAAACTGGCCAGG5150.18417260012373537No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5080.18166928322884965No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC5030.1798811997325027No Hit
CTACCATACTGCTGACAGTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC4900.17523218264200063No Hit
GTACATGGGTGACCTACCATACTGCTGACAGTAATATACTGCAAAATCTT4650.16629176516026592No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4560.16307321486684143No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4480.1602122812726863No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACATGGGTGACCTACCATACTG4430.15842419777633937No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACC4340.15520564748291488No Hit
GCAGAAAACTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAAGAGGGCCACTGGCATCCCAGACAGGTT4260.15234471388875975No Hit
AAGTAGGTACTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTG4210.15055663039241282No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC4180.14948378029460463No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG4170.14912616359533526No Hit
TCTATGGTGCATCCAAGAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT4140.14805331349752707No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4080.14590761330191074No Hit
CCTCATCTATGGTGCATCCAAGAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT4080.14590761330191074No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC4070.14554999660264137No Hit
GTGTACATGGGTGACCTACCATACTGCTGACAGTAATATACTGCAAAATC4060.14519237990337197No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT3940.14090097951213928No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA3930.1405433628128699No Hit
GGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGA3890.13911289601579235No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC3830.13696719582017602No Hit
GAGTACGGGAGAAGAGCTGCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC3730.13339102882748213No Hit
ATGGTAGGTCACCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT3720.13303341212821274No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT3720.13303341212821274No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG3710.13267579542894337No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGTACCTACTTA3680.13160294533113517No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA3660.1308877119325964No Hit
CCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCAC3600.12874201173698008No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG3580.1280267783384413No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC3580.1280267783384413No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC3530.12623869484209435No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGG3440.12302014454866986No Hit
GTTTTCTGCTGGTACCAGGCTAAGTAGGTACTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC3430.12266252784940046No Hit
GTATCAACGCAGAGTACGGGAGAAGAGCTGCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTT3390.1212320610523229No Hit
GGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGTAC3390.1212320610523229No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTT3360.12015921095451473No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG3360.12015921095451473No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA3360.12015921095451473No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC3350.11980159425524534No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC3350.11980159425524534No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC3330.11908636085670656No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA3330.11908636085670656No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT3300.1180135107588984No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA3300.1180135107588984No Hit
GTACGGGGAAGAGCTGCTCAGTTAGGACCCAGAGGGAACCATGGAAACCC3290.11765589405962902No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCAAGAGGGCCACTGGCATCCCA3260.11658304396182084No Hit
ACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGTACCTACTTAGCCTGGTACCAGCAGAAAACTGGCCAGGCT3260.11658304396182084No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT3260.11658304396182084No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT3220.11515257716474328No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG3210.1147949604654739No Hit
ACCATACTGCTGACAGTAATATACTGCAAAATCTTCAGGCTCCAGTCTGC3210.1147949604654739No Hit
GAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC3150.11264926026985757No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT3140.11229164357058818No Hit
ATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACG3120.1115764101720494No Hit
CTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGA3090.11050356007424122No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGG3070.10978832667570244No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGT3050.10907309327716366No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC3040.10871547657789428No Hit
CACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAA3030.10835785987862488No Hit
GCCTGGTACCAGCAGAAAACTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAAGAGGGCCACTGGCATC3010.1076426264800861No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA2980.10656977638227795No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGAC2920.10442407618666161No Hit
GCCTGGGAGCCTGGCCAGTTTTCTGCTGGTACCAGGCTAAGTAGGTACTG2860.10227837599104526No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG2850.10192075929177588No Hit
CATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG2840.10156314259250651No Hit
ATCCAAGAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAG2830.10120552589323711No Hit
GGCTAAGTAGGTACTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGG2800.10013267579542895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT502.1827873E-1160.4530071
TTTCGAT451.6580385E-460.267570
GCTTGTA409.92186E-750.3775021
CCGTTAA200.00820077150.34147316
AAAGGGG2250.049.18884368
TCCGGGG403.213697E-546.4629166
ACAGGTA752.3646862E-1144.7800031
ACTAGCG2350.041.58672327
GACTAGC2350.041.5270726
ACGAGAC2350.041.4453222
ATGCCGT2300.041.11828644
TAGCGCT2400.040.88183229
TCTCCGA2300.040.8641511
CTAGCGC2400.040.78624728
AGACTAG2400.040.6619225
GAGACTA2400.040.6327824
CGAGACT2400.040.5964123
CACGAGA2400.040.57461521
CGCTCAT2350.040.52380832
GACAGCC504.6098194E-640.3020061
Produced by FastQC (version 0.11.5)