FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279629
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20000.7152333985387782No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14130.5053123960676468No Hit
GTATATTACTGTCAGCAGTATGGTAGGTCACCCATGTACACTTTTGGCCA12320.44058377349988737No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA12200.43629237310865465No Hit
GTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11580.41412013775395257No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11570.41376252105468314No Hit
ATATTACTGTCAGCAGTATGGTAGGTCACCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA10890.3894445855043647No Hit
CAGTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9970.35654384917158094No Hit
GTATGGTAGGTCACCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT9540.3411663311029972No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9250.3307954468241849No Hit
CATCTATGGTGCATCCAAGAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA8990.32149741264318077No Hit
GTGTTAGCAGTACCTACTTAGCCTGGTACCAGCAGAAAACTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT8170.29217284330309085No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGTACCTACTTAGCCTGGTACC7540.2696429912491194No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGTTTTCTGCTGGTACCAGGCTAAGTAGGTACTGCTAACACTCTGA7510.2685701411513112No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA7510.2685701411513112No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTCTT6930.24782837259368662No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6910.24711313919514788No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT6900.24675552249587848No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6710.23996080520976007No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT6660.2381727217134131No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6630.23709987161560495No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6410.2292323042316784No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6370.22780183743460083No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6310.22565613723898453No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGTA6210.22207997024629061No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6180.22100712014848245No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA6130.2192190366521355No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6110.21850380325359672No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6110.21850380325359672No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACATGGGTGA5720.20455675198209053No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTATATTACTGTCAGCAGTATGGTAGGTCA5700.20384151858355176No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT5650.20205343508720486No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5590.1999077348915885No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA5570.19919250149304973No Hit
GTCAGAGTGTTAGCAGTACCTACTTAGCCTGGTACCAGCAGAAAACTGGC5520.19740441799670277No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC5510.19704680129743338No Hit
CCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC5500.19668918459816398No Hit
CTACCATACTGCTGACAGTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC5210.1863183003193517No Hit
GGCCAAAAGTGTACATGGGTGACCTACCATACTGCTGACAGTAATATACTGCAAAATCTTCAGGCTCCAGTCTG4990.17845073293542515No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT4910.17558979934127003No Hit
GTACGGGAGAAGAGCTGCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTG4830.17272886574711493No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG4740.1695103154536904No Hit
GAGTGTTAGCAGTACCTACTTAGCCTGGTACCAGCAGAAAACTGGCCAGG4700.16807984865661285No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC4690.16772223195734348No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC4660.16664938185953532No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4590.1641460649646496No Hit
GTACATGGGTGACCTACCATACTGCTGACAGTAATATACTGCAAAATCTT4580.1637884482653802No Hit
GTACCTACTTAGCCTGGTACCAGCAGAAAACTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAAGAGGG4390.1569937309792618No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACATGGGTGACCTACCATACTG4380.15663611427999244No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT4220.1509142470916822No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC4170.14912616359533526No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACC4130.1476956967982577No Hit
GAGTACGGGAGAAGAGCTGCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC4120.1473380800989883No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4070.14554999660264137No Hit
GTGTACATGGGTGACCTACCATACTGCTGACAGTAATATACTGCAAAATC4030.1441195298055638No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT4020.1437619131062944No Hit
AAGTAGGTACTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTG4010.143404296407025No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG3920.14018574611360052No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA3920.14018574611360052No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC3840.1373248125194454No Hit
TCTATGGTGCATCCAAGAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT3790.13553672902309846No Hit
CCTCATCTATGGTGCATCCAAGAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT3740.13374864552675153No Hit
GCAGAAAACTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAAGAGGGCCACTGGCATCCCAGACAGGTT3660.1308877119325964No Hit
GTTTTCTGCTGGTACCAGGCTAAGTAGGTACTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC3620.12945724513551884No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG3570.1276691616391719No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTT3540.12659631154136375No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA3520.12588107814282495No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC3510.12552346144355556No Hit
ATGGTAGGTCACCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT3470.12409299464647801No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA3460.12373537794720864No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGTACCTACTTA3450.12337776124793924No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC3410.12194729445086168No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGG3390.1212320610523229No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA3390.1212320610523229No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT3370.12051682765378412No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC3350.11980159425524534No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGT3330.11908636085670656No Hit
ATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACG3330.11908636085670656No Hit
GGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGTAC3310.11837112745816777No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC3280.11729827736035962No Hit
ACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGTACCTACTTAGCCTGGTACCAGCAGAAAACTGGCCAGGCT3260.11658304396182084No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT3240.11586781056328206No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC3220.11515257716474328No Hit
CTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGA3170.11336449366839634No Hit
GTACGGGGAAGAGCTGCTCAGTTAGGACCCAGAGGGAACCATGGAAACCC3140.11229164357058818No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA3120.1115764101720494No Hit
GTATCAACGCAGAGTACGGGAGAAGAGCTGCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTT3110.11121879347278No Hit
ACCATACTGCTGACAGTAATATACTGCAAAATCTTCAGGCTCCAGTCTGC3110.11121879347278No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA3090.11050356007424122No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG3090.11050356007424122No Hit
GCCTGGGAGCCTGGCCAGTTTTCTGCTGGTACCAGGCTAAGTAGGTACTG3080.11014594337497184No Hit
CCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCAC3080.11014594337497184No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT3060.10943070997643306No Hit
GAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC3040.10871547657789428No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGG3010.1076426264800861No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC3000.10728500978081673No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG2990.10692739308154735No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC2940.10513930958520039No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC2930.104781692885931No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT2930.104781692885931No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC2910.10406645948739222No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC2890.10335122608885344No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG2880.10299360938958405No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC2840.10156314259250651No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT2810.10049029249469833No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)