FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402292
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26600.6612112594831615No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATAGA14270.35471746890318473No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12270.30500233661121773No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12250.30450518528829806No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11070.2751732572360375No Hit
GTATAATAATTTACTTAGTTTTGGCGGAGGGACCATGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT9130.22694957891282946No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8880.22073518737633363No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8730.21700655245443606No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG8600.21377506885545822No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8390.2085549799648017No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8310.206566374673123No Hit
GTATATTACTGTGCGAAAACCGCGGGTTACTATGATAGTAGTCGTTATTC8060.20035198313662714No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7120.1769858709594026No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7120.1769858709594026No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7080.17599156831356327No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6960.17300866037604526No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT6520.16207133127181253No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGATCTGGGTCCGCCAGGCTCCAGGGAAGGGG6490.161325604287433No Hit
TTATTATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAGTCTGTC6190.15386833444363796No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6040.15013969952174042No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6020.14964254819882075No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5920.1471567915842224No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT5630.13994809740188718No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC5460.13572231115706998No Hit
ATATTACTGTCAACAGTATAATAATTTACTTAGTTTTGGCGGAGGGACCATGGTGGAGATCAAACGAACTGTGG5390.13398228152685113No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5320.1322422518966323No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG5190.12901076829765445No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAAAAA5160.12826504131327493No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG5080.12627643602159624No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT5030.12503355771429708No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA4970.12354210374553806No Hit
GTCCCCATCCTCCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAAAAA4940.12279637676115855No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG4890.12155349845385938No Hit
GTATTAGGGCTAGTGGTAGTGGCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATT4870.12105634713093971No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA4720.11732771220904219No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA4710.11707913654758234No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAGTCTGTCCCAGATCCACTTCCA4670.11608483390174301No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAGTCTGTCCCAGATCCACTTCCA4640.1153391069173635No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4500.11185904765692581No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT4320.10738468575064877No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG4200.10440177781313076No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC4120.10241317252145207No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC4070.1011702942141529No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG4070.1011702942141529No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCTA150.001807041273.75883564
TCTGGAG5300.051.84649370
GTGGTAT1250.037.6625061
TAGGCAA1453.8273356E-836.97714270
AGAGCGT4700.035.17603748
AATAGTC400.002675442534.2839236
GGAATTG4900.034.2246761
CGTGTTC4900.033.99682652
GCGTGTT4900.033.94342851
TAGAGCG4900.033.66144647
GCAGATA5000.033.35241342
ATTGTCT5100.033.26378664
ATAGAGC5000.032.9668246
TGTCTCT5150.032.674966
TCGGCTC5050.032.66904420
TGGAATT5150.032.462660
AATTGTC5200.032.4602763
CGGCTCT5100.032.35278721
TCTTGGA5150.032.15569757
GAATTGT5300.031.75300262
Produced by FastQC (version 0.11.5)