FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071300
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCTCACTTTCGGCGGCGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT39810.3716045925511061No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34810.3249323252123588No Hit
GTTATTATACTGCTGACAGGAATAAACTGCAAAATCTTCAGACTGTAGGCTGTTGATGGTGAGAGTGAATT33080.30878372071315224No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32470.30308970409782504No Hit
CTCCTGTACAGCCTCTGGATTCACCTTCAGTGGCTACTGGATGCACTGGGTCCGCCAAGCTCCAGGGAATGGA32160.3001960235228227No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28770.268552226267152No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23640.2206664799775973No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23080.21543918603565762No Hit
CATCTATGGTGTATCCACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCAGGGACAGAATTCA21370.19947727060580603No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21190.19779706898161112No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20460.190982917950154No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20360.19004947260337907No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG19900.18575562400821433No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19420.18127508634369457No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18990.1772612713525623No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC18760.17511434705497994No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18670.17427424624288249No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT18550.17315411182675253No Hit
ATAATACACAGCCGTGTCCTCGACTCTCAGACTGTTCATTTCCAGATACA18460.1723140110146551No Hit
CAATAATACACAGCCGTGTCCTCGACTCTCAGACTGTTCATTTCCAGATA18240.1702604312517502No Hit
GTATTATTGTGTAAGAGAGGCGCCGGGGCGTGGGAACTGGTATAGTGACTACTGGGGCCAGGGAACCCTGGTCAC18160.16951367497433026No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18140.16932698590497527No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18130.16923364137029778No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17940.16746009521142538No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17890.1669933725380379No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17520.16353962475497058No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT17510.1634462802202931No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG17380.1622328012694857No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17070.15933912069448333No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16880.15756557453561093No Hit
GTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTA16340.15252496966302623No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16250.1516848688509288No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16110.15037804536544386No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15910.14851115467189396No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15650.1460841967702791No Hit
GAGTAGAACAAGTCACGCGGACTCCGTGAAGGGCCGATTCAGCATCTCCA15540.14505740688882668No Hit
GTCTGAGAGTCGAGGACACGGCTGTGTATTATTGTGTAAGAGAGGCGCCG15470.1444039951460842No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC15460.14431065061140672No Hit
CTATACCAGTTCCCACGCCCCGGCGCCTCTCTTACACAATAATACACAGC15400.14375058340334174No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15380.14356389433398675No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14730.1374964995799496No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT14700.1372164659759171No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14670.13693643237188463No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14640.13665639876785213No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14620.13646970969849714No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14560.13590964249043216No Hit
GGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC14550.13581629795575467No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC14470.13506954167833474No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14280.13329599551946233No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14250.13301596191542986No Hit
GCCCTGGGATTCCCAGCTGTTTCTGCTTGCTGATCAGGACTGCACACAGA13910.12984224773639502No Hit
TCTATGGTGTATCCACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCAGGGACAGAATTCACT13610.1270419116960702No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG13380.12489498739848783No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13210.12330813030897042No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13180.12302809670493792No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG13120.12246802949687295No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12870.12013441612993558No Hit
GTGTGAGGTTCAGCTGGTGGAGTCCGGGGGAGGCTTAGTTCACCCTGGGGGGTCCCTGAGACTCTCCTGTACAGC12760.11910762624848314No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12740.11892093717912816No Hit
GAATTCACTCTCACCATCAACAGCCTACAGTCTGAAGATTTTGCAGTTTATTCCTGTCAGCAGTATAATAACTGG12730.11882759264445067No Hit
GTGTATTATTGTGTAAGAGAGGCGCCGGGGCGTGGGAACTGGTATAGTGA12710.11864090357509567No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12530.11696070195090078No Hit
GTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAG12510.11677401288154579No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12470.1164006347428358No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12420.11593391206944834No Hit
GGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAAAC12340.11518715579202839No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12250.11434705497993093No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12150.11341360963315597No Hit
CTCTTACACAATAATACACAGCCGTGTCCTCGACTCTCAGACTGTTCATT11960.11164006347428358No Hit
CTGGTATAGTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTT11830.11042658452347615No Hit
CCAGTGGCCCTGGTGGATACACCATAGATGAGGAGCCTGGGAGCCTGGCC11800.11014655091944367No Hit
GGTGTGGGTCTCACGTATTTATGATGATGGGAGTAGAACAAGTCACGCGGACTCCGTGAAGGGCCGATTCAGCAT11670.10893307196863623No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT11390.10631942499766639No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11380.1062260804629889No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11340.1058527023242789No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11340.1058527023242789No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11320.10566601325492392No Hit
CTCTCAGACTGTTCATTTCCAGATACAGCGTGTTCCTGGCGTTGTCTCTG11240.10491925697750397No Hit
GAGTGAATTCTGTCCCTGACCCACTGCCACTGAACCTGGCTGGGACACCA11110.10370577802669653No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCGCCGCCGAAAGTG10960.10230561000653413No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCA10920.10193223186782413No Hit
GTGTAAGAGAGGCGCCGGGGCGTGGGAACTGGTATAGTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCT10900.10174554279846915No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10870.10146550919443666No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10860.10137216465975916No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)