FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751952
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCAT39800.5292891035598016No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38610.5134636253377874No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35390.47064174308998447No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30680.40800476626167625No Hit
GTTATTATACTGCTGACAGAAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGTGTGAACT27640.3675766538289678No Hit
GAGTTCACACTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTTCTGTCAGCAGTATAATAACTGG27500.365714832861672No Hit
GTCTGGGACAGAGTTCACACTCACCATCAGCAGCCTGCAGTCTGAAGATT23000.3058705874843075No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22900.30454071536481053No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21580.2869864033874503No Hit
GTCTGTGGCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTGGCCTGGTG21110.2807360044258144No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20370.2708949507415367No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20330.2703630018937379No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20080.2670383215949954No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19680.2617188331170075No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18190.24190373853650235No Hit
GTGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGTGGATGCACCATAGA18140.24123880247675383No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18020.23964295593335744No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGT17920.2383130838138605No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17730.23578632678681616No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTGGCCTGGTGCCAGCAGAAACCTGGCCAGGCTCCCAGGCT17280.2298019022490797No Hit
GTCTCCAGCCACCCTGTCTGTGGCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG17200.22873800455348214No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC15730.20918888439687638No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15640.2079919994893291No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15230.20253952379939147No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14950.19881588186479987No Hit
CCACTGGAGAGGTAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGGCTCCAGGGGAAGGAGCCACCCTCTC14910.1982839330170011No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGTC14550.1934963933868119No Hit
CGTCTATGGTGCATCCACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTG13560.18033065940379173No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13540.18006468497989234No Hit
CCATAGACGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGCACCAGGC13520.17979871055599292No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13490.17939974892014382No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT13410.17833585122454626No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12780.1699576568717152No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12650.16822882311636914No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12550.16689895099687213No Hit
GTCAGAGTGTTAGCAGCAACTTGGCCTGGTGCCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCGTCTATGGTG12480.16596804051322425No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11940.1587867310679405No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11660.15506308913334893No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11550.15360022980190224No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11490.15280230653020405No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11120.14788177968806518No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10800.14362618890567483No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10640.14149839351447965No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10540.14016852139498265No Hit
CCAGTGGCCCTGGTGGATGCACCATAGACGAGGAGCCTGGGAGCCTGGCC10360.13777475157988808No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT10360.13777475157988808No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10350.1376417643679384No Hit
GGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGT10120.1345830584930953No Hit
GTGTTAGCAGCAACTTGGCCTGGTGCCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCGTCTATGGTGCATCCA9850.13099240377045343No Hit
CTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACA9790.13019448049875526No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT9620.12793369789561035No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9600.12766772347171096No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAGGTAGTGATGACGCAGTC9520.12660382577611337No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9510.12647083856416366No Hit
CACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTTCTGTCAGCAGTATAATAACTGGCCTCCGACGTT9310.1238110943251697No Hit
CACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACACTCACCATCAGCAG9170.12194927335787392No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9100.12101836287422602No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9080.12075238845032663No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9070.12061940123837692No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9020.11995446517862843No Hit
GAGTGTTAGCAGCAACTTGGCCTGGTGCCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCGTCTATGGTGCATC8780.11676277209183565No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8750.11636381045598655No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8750.11636381045598655No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC8680.11543289997233866No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC8590.11423601506479136No Hit
CTCCCAGGCTCCTCGTCTATGGTGCATCCACCAGGGCCACTGGTGTCCCA8560.11383705342894226No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8550.11370406621699258No Hit
GGTAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGGCTCCAGGGGAAG8530.11343809179309317No Hit
ACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTGGCCTGGTG8500.11303913015724408No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8480.11277315573334469No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTGGCCTGGTGCCAGCAGAAACCTGGCCAGG8430.1121082196735962No Hit
CCCAGATACCACTGGAGAGGTAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGGCTCCAGGGGAAGGAGCCAC8290.1102463987063004No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8270.109980424282401No Hit
GGAGAGGTAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGGCTCCAGG8200.1090495137987531No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8180.10878353937485372No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAGGTAGTGATGACGCAGTCTC8160.10851756495095433No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8130.10811860331510523No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACACTCACCATCAGCAGCC8090.10758665446730642No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8050.10705470561950761No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8030.10678873119560822No Hit
GCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGTCGGAGG8010.10652275677170883No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT7980.10612379513585973No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA7960.10585782071196033No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7870.10466093580441306No Hit
TTATTATACTGCTGACAGAAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGTGTGAACTCTGTC7810.10386301253271486No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGT7800.10373002532076515No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC7760.10319807647296636No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7710.10253314041321787No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7700.10240015320126816No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGTCGGAGGCCAGTT7580.10080430665787177No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)