FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751952
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41930.5576153797050876No Hit
GTATAATAACTGGCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCAT40040.5324807966465944No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30950.41159542098431817No Hit
GTTATTATACTGCTGACAGAAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGTGTGAACT29470.3919133136157627No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28900.38433304253462985No Hit
GAGTTCACACTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTTCTGTCAGCAGTATAATAACTGG26120.34736259761261357No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23550.31318488414154094No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20920.2782092473987701No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20840.27714534970317256No Hit
GTCTGGGACAGAGTTCACACTCACCATCAGCAGCCTGCAGTCTGAAGATT20750.27594846479562524No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19570.26025597378556076No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19460.2587931144541141No Hit
GTGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGTGGATGCACCATAGA19070.25360661318807587No Hit
GTCTGTGGCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTGGCCTGGTG18990.25254271549247825No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18370.2442975083515969No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTGGCCTGGTGCCAGCAGAAACCTGGCCAGGCTCCCAGGCT17570.23365853139562098No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGT17340.2305998255207779No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17200.22873800455348214No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16920.22501436261889057No Hit
GTCTCCAGCCACCCTGTCTGTGGCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG15490.20599719131008362No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15350.20413537034278786No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15350.20413537034278786No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15350.20413537034278786No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15320.20373640870693877No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC15290.20333744707108967No Hit
CCACTGGAGAGGTAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGGCTCCAGGGGAAGGAGCCACCCTCTC15030.19987977956039746No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14720.19575717598995682No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14630.19456029108240952No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14250.18950677702832097No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGTC14040.18671404557737728No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT13960.1856501478817797No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13300.17687299189309957No Hit
CGTCTATGGTGCATCCACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTG12530.16663297657297274No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12260.16304232185033088No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12130.16131348809498477No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12120.1611805008830351No Hit
CCATAGACGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGCACCAGGC11900.15825478222014172No Hit
GTCAGAGTGTTAGCAGCAACTTGGCCTGGTGCCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCGTCTATGGTG11590.15413217864970105No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11410.15173840883460646No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10960.14575398429687No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10640.14149839351447965No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10600.14096644466668085No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10550.14030150860693236No Hit
CCAGTGGCCCTGGTGGATGCACCATAGACGAGGAGCCTGGGAGCCTGGCC10350.1376417643679384No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10340.1375087771559887No Hit
GTGTTAGCAGCAACTTGGCCTGGTGCCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCGTCTATGGTGCATCCA9900.13165733983020192No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9830.13072642934655404No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA9720.12926357001510735No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT9630.12806668510756006No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAGGTAGTGATGACGCAGTC9410.12514096644466668No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9330.12407706874906908No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGTCGGAGGCCAGTT9130.12141732451007511No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9020.11995446517862843No Hit
CACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTTCTGTCAGCAGTATAATAACTGGCCTCCGACGTT9000.11968849075472902No Hit
CTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACA8910.11849160584718173No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8760.11649679766793625No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8570.11397004064089197No Hit
GGTAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGGCTCCAGGGGAAG8560.11383705342894226No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8440.11224120688554588No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGGCACCAGGCCAAGTTGCTGCTA8410.11184224524969678No Hit
GGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGT8360.1111773091899483No Hit
ACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTGGCCTGGTG8350.11104432197799859No Hit
TTATTATACTGCTGACAGAAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGTGTGAACTCTGTC8350.11104432197799859No Hit
GAGTGTTAGCAGCAACTTGGCCTGGTGCCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCGTCTATGGTGCATC8350.11104432197799859No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8330.1107783475540992No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8320.11064536034214949No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT8290.1102463987063004No Hit
GCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGTCGGAGG8120.10798561610315552No Hit
CACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACACTCACCATCAGCAG8050.10705470561950761No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAGGTAGTGATGACGCAGTCTC8010.10652275677170883No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7970.10599080792391004No Hit
CTCCCAGGCTCCTCGTCTATGGTGCATCCACCAGGGCCACTGGTGTCCCA7920.10532587186416155No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC7870.10466093580441306No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7840.10426197416856396No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACACTCACCATCAGCAGCC7840.10426197416856396No Hit
GGAGAGGTAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGGCTCCAGG7810.10386301253271486No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7770.10333106368491606No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC7760.10319807647296636No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTGGCCTGGTGCCAGCAGAAACCTGGCCAGG7700.10240015320126816No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA7640.10160222992956996No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7620.10133625550567057No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC7590.10093729386982148No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7560.10053833223397238No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7560.10053833223397238No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT7530.10013937059812328No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)