FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712820
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27720.3888779776100558No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26450.37106141802979714No Hit
GACTTATAGTACCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT22570.31662972419404617No Hit
GAGTTCACCCTCACCATCAGCAATTTGCAACCTGAAGACTTTGGAATTTATTACTGTCAACAGACTTATAGTACC19320.27103616621306925No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18260.2561656519177352No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT16790.23554333492326254No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16610.23301815325046998No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16510.231615274543363No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16090.22572318397351365No Hit
CTATAAGTCTGTTGACAGTAATAAATTCCAAAGTCTTCAGGTTGCAAATTGCTGATGGTGAGGGTGAACTCTGTG15860.2224965629471676No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15490.21730591173087177No Hit
GTCTGGCACAGAGTTCACCCTCACCATCAGCAATTTGCAACCTGAAGACTTTGGAATTTATTACTGTCAACAGA15410.21618360876518616No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14850.20832748800538706No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14470.20299654891838054No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14370.20159367021127356No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13930.1954210039000028No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCCTCAGGGAGA13820.1938778373221851No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13640.19135265564939255No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12310.1726943688448697No Hit
GTGCCAGACCGACTGCCACTGAACCTTGACGGGACCCCATCTTGCAAATT11500.1613310513173031No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11170.15670155158385005No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11090.15557924861816447No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG10930.1533346426867933No Hit
GTGGGAGACAGAGTCATCATCACTTGCCGGGCAAGTCTGACCATTCACAACTATTTAAACTGGTATCAGAAGAGA10780.1512303246261328No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10780.1512303246261328No Hit
GGGTGAACTCTGTGCCAGACCGACTGCCACTGAACCTTGACGGGACCCCA10660.14954687017760443No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10640.14926629443618306No Hit
ATAGTACCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA10630.14912600656547234No Hit
CTCCTGATCTATGCCGCATCCAATTTGCAAGATGGGGTCCCGTCAAGGTT10420.1461799612805477No Hit
CTGCTACTCTGGCTCCGAAGTGCCAGGTGTGACATCCAGATGACCCAGTC10360.1453382340562835No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC10220.1433742038663337No Hit
GTCTGCATCTGTGGGAGACAGAGTCATCATCACTTGCCGGGCAAGTCTGA10170.14267276451278024No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAAGTGCCAGGTGTGACAT10050.14098931006425183No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9890.13874470413288068No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9720.1363598103307988No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9440.13243174995089926No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9400.13187059846805646No Hit
GATCAGGAGTTTAAAGGGATTCCCTGGTCTCTTCTGATACCAGTTTAAAT9050.126960522993182No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8980.1259785078982071No Hit
CCCTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCATCATCACTTGCCG8930.12527706854465365No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8900.12485620493252153No Hit
GTTCAGTGGCAGTCGGTCTGGCACAGAGTTCACCCTCACCATCAGCAATT8770.12303246261328245No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8330.11685979630201174No Hit
GATCTATGCCGCATCCAATTTGCAAGATGGGGTCCCGTCAAGGTTCAGTGGCAGTCGGTCTGGCACAGAGTTCAC8210.11517634185348334No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8080.11335259953424426No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8060.11307202379282287No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8020.11251087230998008No Hit
GTCTCCCTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCATCATCACTTGCCGGGCAAGTCTGACCATTCACAA7970.11180943295642659No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7950.11152885721500518No Hit
CTTATAGTACCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA7930.1112482814735838No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7780.10914396341292332No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7720.10830223618865914No Hit
GTCTATTACTGTGCGAGGGGTCGTTGTAGTGGTAATAACTGCTACCTCTA7600.10661878174013076No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7590.10647849386942006No Hit
GGGTACTATAAGTCTGTTGACAGTAATAAATTCCAAAGTCTTCAGGTTGCAAATTGCTGATGGTGAGGGTGAACT7560.10605763025728797No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7450.10451446367947027No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7380.1035324485844954No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAAGTGCCAGGTGTGACATCCAGATGACCCAGTCTCCCTCCTCC7380.1035324485844954No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC7180.10072669117028141No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)