FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712820
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30990.43475211133245417No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26310.36909738783984736No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22210.31157936084846105No Hit
GACTTATAGTACCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT21610.3031620886058191No Hit
GAGTTCACCCTCACCATCAGCAATTTGCAACCTGAAGACTTTGGAATTTATTACTGTCAACAGACTTATAGTACC19740.27692825678291855No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17480.24522319800230072No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16740.23484189556970905No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15950.22375915378356387No Hit
GTCTGGCACAGAGTTCACCCTCACCATCAGCAATTTGCAACCTGAAGACTTTGGAATTTATTACTGTCAACAGA15400.21604332089447545No Hit
CTATAAGTCTGTTGACAGTAATAAATTCCAAAGTCTTCAGGTTGCAAATTGCTGATGGTGAGGGTGAACTCTGTG15370.21562245728234336No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14730.2066440335568587No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14020.19668359473639907No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCCTCAGGGAGA13290.18644258017451812No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13180.18489941359670042No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13040.18293538340675067No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11920.16722314188715245No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11920.16722314188715245No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11810.16567997530933476No Hit
GTGGGAGACAGAGTCATCATCACTTGCCGGGCAAGTCTGACCATTCACAACTATTTAAACTGGTATCAGAAGAGA11690.16399652086080638No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11510.1614713391880138No Hit
GTGCCAGACCGACTGCCACTGAACCTTGACGGGACCCCATCTTGCAAATT11160.15656126371313936No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10940.153474930557504No Hit
CTCCTGATCTATGCCGCATCCAATTTGCAAGATGGGGTCCCGTCAAGGTT10930.1533346426867933No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10720.15038859740186863No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10710.15024830953115795No Hit
GGGTGAACTCTGTGCCAGACCGACTGCCACTGAACCTTGACGGGACCCCA10570.14828427934120816No Hit
GTCTGCATCTGTGGGAGACAGAGTCATCATCACTTGCCGGGCAAGTCTGA10540.14786341572907608No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10410.14603967340983698No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10400.1458993855391263No Hit
CTGCTACTCTGGCTCCGAAGTGCCAGGTGTGACATCCAGATGACCCAGTC10200.14309362812491233No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC10190.14295334025420162No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAAGTGCCAGGTGTGACAT10110.14183103728851604No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10100.14169074941780532No Hit
ATAGTACCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA9830.1379029769086165No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG9540.13383462865800622No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9260.12990656827810668No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9260.12990656827810668No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9080.12738138660531412No Hit
GTTCAGTGGCAGTCGGTCTGGCACAGAGTTCACCCTCACCATCAGCAATT8950.12555764428607502No Hit
GATCAGGAGTTTAAAGGGATTCCCTGGTCTCTTCTGATACCAGTTTAAAT8800.12345332622541456No Hit
GTCTCCCTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCATCATCACTTGCCGGGCAAGTCTGACCATTCACAA8460.11868353862125082No Hit
CCCTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCATCATCACTTGCCG8290.11629864481916892No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8120.11391375101708705No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8100.11363317527566567No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7920.11110799360287309No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7850.11012597850789821No Hit
GATCTATGCCGCATCCAATTTGCAAGATGGGGTCCCGTCAAGGTTCAGTGGCAGTCGGTCTGGCACAGAGTTCAC7830.10984540276647681No Hit
CTTATAGTACCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA7530.10563676664515585No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7500.10521590303302376No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7420.10409360006733819No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7220.1012878426531242No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAAGTGCCAGGTGTGACATCCAGATGACCCAGTCTCCCTCCTCC7220.1012878426531242No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)