FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739115
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90791.2283609451844435No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA27340.36990184206787846No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21550.2915649120908113No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20220.27357041867639004No Hit
ATATTACTGTCAGCACTATGGTAGCCCCCGGACGTTCGGCCAAGGGTCCAAGGTGGAAATCAAACGAACTGTGG15680.21214560657001955No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15070.20389249304911955No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA14640.1980747245015999No Hit
GTATATTACTGTCAGCACTATGGTAGCCCCCGGACGTTCGGCCAAGGGTC14500.19618056730008188No Hit
GTGTATTACTGTGCAAGAGATAATTCTAGTAGGAATGGTACGTGTATGGA14190.19198636206814906No Hit
GTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCG13630.18440973326207696No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA13290.17980963720124743No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12850.17385657171076221No Hit
CAGTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCG11930.1614092529579294No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC11760.15910920492751465No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10790.145985401459854No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC10680.1444971350872327No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10650.144091244258336No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT10290.13922055431157532No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG10120.1369205062811606No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT10060.13610872462336715No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT9990.1351616460226081No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9920.1342145674218491No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9830.13299689493515895No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTCAGTAGCTATAACATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG9720.13150862856253762No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT9720.13150862856253762No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9710.13137333161957204No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACACGTTCAGCGGCAGTGGGTCTGGGACAGACTTCA9610.13002036218991633No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTAGCAGCAGCTTCTTAGCCTGGTACC9340.12636734472984584No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9180.12420259364239665No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT9010.12190254561198188No Hit
CTCCAGGCACCCTGTCATTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC8820.11933190369563601No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC8650.11703185566522124No Hit
GTCTCCAGGCACCCTGTCATTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTAGCAG8620.11662596483632452No Hit
TCCATACACGTACCATTCCTACTAGAATTATCTCTTGCACAGTAATACACAGCCGTGTCCTCGGCTCTCAGG8550.11567888623556552No Hit
ATGGTAGCCCCCGGACGTTCGGCCAAGGGTCCAAGGTGGAAATCAAACGA8430.11405532291997862No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8300.11229646266142618No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC8210.111078790174736No Hit
CCCTGGGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATACACG8190.11080819628880485No Hit
GTCCCAGACCCACTGCCGCTGAACGTGTCTGGGATGCCAGTGGCCCTGCT8120.10986111768804584No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8110.10972582074508026No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8080.10931992991618356No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC7910.10701988188576879No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGAAGCTGCTGCTAAGACTCTGA7840.10607280328500977No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC7660.10363745831162945No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7560.10228448888197372No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT7510.10160800416714585No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7500.10147270722418025No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC7470.10106681639528355No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGG100.008508391136.4122370
AAACGGT200.007652170551.239336
TGGTATC1400.047.858162
GTGGTAT1600.046.097791
TCTGGAG8750.043.65191770
GGTATCA5550.043.4927561
GACAGGT950.042.348211
GTATCAA13600.035.4977651
TCCGAGA5250.033.77826770
TATCAAC15450.031.007132
ACAGGTA652.8221584E-530.946771
GTGCGTC553.8757856E-430.444893
ACAGACG5700.029.91496370
AAGTGCG450.005339556429.77437814
TCCCACG450.005343090329.7703511
GACAGCC1152.3119355E-929.1527541
GCAGATA8000.029.10262342
ATCAACG16600.029.0510253
TCAACGC16950.028.4511534
CAACGCA17250.027.9582425
Produced by FastQC (version 0.11.5)